TM4SF1/L6 Antibody

Western blot shows lysates of LL/2 mouse Lewis lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse TM4SF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7514) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TM4SF1 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TM4SF1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse TM4SF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7514) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to the trabeculae of the developing heart. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

TM4SF1/L6 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse TM4SF1
  Ala116-Thr161
  Accession # Q64302
• Specificity: Detects mouse TM4SF1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TM4SF1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TM4SF1/L6 Antibody

• L6
• M3s1
• M3S1Tumor-associated antigen L6
• Membrane component chromosome 3 surface marker 1
• membrane component, chromosome 3, surface marker 1
• TAAL6
• TAAL6H-L6
• TM4SF1
• transmembrane 4 L six family member 1
• transmembrane 4 L6 family member 1
• transmembrane 4 superfamily member 1

Background

TM4SF1 (Transmembrane 4 L6 family Member 1; also L6 and M3s1) is a 23-28 kDa member of the L6 tetraspanin family of molecules. It is expressed by fibroblasts, endothelial cells and a variety of tumor cells. TM4SF1 is embedded in both the plasma membrane and the membrane of late endocytic organelles. Here, it appears to be ubiquitinated, and to regulate endocytosis, an action that impacts effective cell migration. TM4SF1 is reported to interact with alpha 5 and beta 1 integrins, and this may impact cell motility. It also suppresses the expression of CD63 and CD82, two molecules that impede cell mobility. Mouse TM4SF1 is a 202 amino acid (aa) 4‑transmembrane (TM) glycoprotein. It contains a short N-terminal cytoplasmic region (aa 1-9), followed by four TM regions and another C-terminal cytoplasmic region (aa 183-202). There is one potential isoform variant that shows an alternative start site at Met60. Over aa 116-161, mouse TM4SF1 shares 73% and 96% aa sequence identity with human and rat TM4SF1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TM4SF1/L6 Antibody (AF7514)(2)

Publications using AF7514

• S Hou, X Hao, J Li, S Weng, J Wang, T Zhao, W Li, X Hu, B Deng, J Gu, Q Hang TM4SF1 promotes esophageal squamous cell carcinoma metastasis by interacting with integrin alpha6 Oncogene, 2022-07-14;13(7):609. 2022-07-14 [PMID: 35835740] (Western Blot, Human)
• WJ Zacharias, DB Frank, JA Zepp, MP Morley, FA Alkhaleel, J Kong, S Zhou, E Cantu, EE Morrisey Regeneration of the lung alveolus by an evolutionarily conserved epithelial progenitor Nature, 2018-02-28;555(7695):251-255. 2018-02-28 [PMID: 29489752] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: TM4SF1
• Uniprot:
  • Q64302()