IRS1 Antibody

Images

 
Western Blot: IRS1 Antibody [NB100-82001] - Analysis of extracts from 293 cells using IRS-1 (Ab-636) antibody
Immunocytochemistry/ Immunofluorescence: IRS1 Antibody [NB100-82001] - Staining of methanol-fixed Hela cells using IRS-1 (Ab-636) antibody
Immunohistochemistry-Paraffin: IRS1 Antibody [NB100-82001] - Analysis of breast carcinoma tissue, using IRS-1 (Ab-636) antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

IRS1 Antibody Summary

Immunogen
Peptide sequence around aa.634~638 (P-M-S-P-K) derived from Human IRS-1.
Specificity
IRS-1 (specific to a.a. 636) detects endogenous levels of total IRS-1 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IRS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-82001 in the following applications:

  • WB
    1 publication

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 29684161).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRS1 Antibody

  • HIRS-1
  • insulin receptor substrate 1
  • IRS1
  • IRS-1

Background

Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS1 degradation pathways are not well understood. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS1 phosphorylation and subsequent degradation an attractive therapeutic target. To date there have been four subtypes identified: IRS1, 2, 3 and 4, with IRS1 being widely expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRS1 Antibody (NB100-82001)(2)

We have publications tested in 2 confirmed species: Mouse, Porcine.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Filter By Species
Mouse
(1)
Porcine
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IRS1 Antibody (NB100-82001) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for IRS1 Antibody (NB100-82001)

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Blogs on IRS1.

Signalling Advances in Adiponectin Antibody Research
Adiponectin is an adipocytokine protein that positively regulates metabolism of lipids and glucose by suppressing glucose production from the liver, stimulating insulin sensitivity, and increasing the rate of fatty acid oxidation and glucose uptake. I...  Read full blog post.

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Calculators

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Bioinformatics

Gene Symbol IRS1
Entrez
Uniprot