Glucocorticoid Receptor alpha Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: Glucocorticoid Receptor alpha Antibody [NB300-633] - Analysis of Glucocorticoid Receptor alpha using Glucocorticoid Receptor alpha Polyclonal Antibody shows staining in U251 ...read more
Immunohistochemistry-Paraffin: Glucocorticoid Receptor alpha Antibody [NB300-633] - Both normal and cancer biopsies of deparaffinized Human heart tissues.
Immunocytochemistry/ Immunofluorescence: Glucocorticoid Receptor alpha Antibody [NB300-633] - Analysis of Glucocorticoid Receptor alpha using Glucocorticoid Receptor alpha Polyclonal Antibody shows staining in A2058 ...read more
Immunocytochemistry/ Immunofluorescence: Glucocorticoid Receptor alpha Antibody [NB300-633] - Analysis of Glucocorticoid Receptor alpha using Glucocorticoid Receptor alpha Polyclonal Antibody shows staining in Hela ...read more
Immunohistochemistry-Paraffin: Glucocorticoid Receptor alpha Antibody [NB300-633] - Both normal and cancer biopsies of deparaffinized Human cervical carcinoma tissues.
Immunohistochemistry-Paraffin: Glucocorticoid Receptor alpha Antibody [NB300-633] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: Glucocorticoid Receptor alpha Antibody [NB300-633] - Both normal and cancer biopsies of deparaffinized Human cervical carcinoma tissues.

Product Details

Summary
Reactivity Hu, Mu, Rt, ShSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
LYOPH

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Catalog# & Formulation Size Price

Glucocorticoid Receptor alpha Antibody - BSA Free Summary

Immunogen
Synthetic Peptide: C(755) E I I T N Q I P K Y S N G N I K K(771)
Specificity
Detects GR alpha from human, mouse and rat tissues. This does not detect GR beta.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR3C1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Paraffin 1:20
  • Immunoprecipitation 1:10 - 1:500
  • Simple Western
  • Western Blot 4 ug/ml
Application Notes
Useful in WB, IP, ICC, IF and ChIP. WB: Detects an approx. 90 kDa protein representing GR in HeLa cell lysate.
Simple Western reported by an internal validation. Separated by Size-Jess/Wes, Sally Sue/Peggy Sue, antibody dilution of 1:50. Apparent MW in kDa on Simple Western was 95kDa; matrix was 12-230 kDa.
Control
eEF1A1 Overexpression Lysate
Publications
Read Publications using
NB300-633 in the following applications:

Reactivity Notes

Sheep reactivity reported in scientific literature (see Gupta et all 2003).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 50 ul ultra-pure water to desired concetration.

Alternate Names for Glucocorticoid Receptor alpha Antibody - BSA Free

  • GCR
  • glucocorticoid receptor
  • GRGCCR
  • GRLNuclear receptor subfamily 3 group C member 1
  • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • nuclear receptor subfamily 3, group C, member 1

Background

Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the Glucocorticoid Receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR. The human GR exists in two forms, alpha and beta, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that alpha and beta forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94 kDa GR alpha isoform translocates from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90 kDa GR beta isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GR alpha has a greater affinity for GR response elements (GREs) than GR beta only when in the ligand bound state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glucocorticoid Receptor alpha Antibody (NB300-633)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NR3C1
Entrez
Uniprot