GLI-1 Antibody

Knockdown Validated: GLI-1 Antibody [NB600-600] - Inhibition of Hh signaling in CAFs blocks its effects on lymphangiogenesis in vivo and in vitro. (E) IHC staining for Ki-67, -SMA, Gli-1 and LYVE1 in tumour sections from mice in the two groups. Human CAFs promote lymphangiogenesis in ovarian cancer via the Hh-VEGF-C signaling axis. Oncotarget (2017)

Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Analysis of a FFPE mouse brain section using GLI-1 antibody at 1:600 dilution.

Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Staining in canine hair follicle. Image from a verified customer review.

Flow (Intracellular): GLI-1 Antibody [NB600-600] - Staining for GLI-1 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-1 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a negative control. Image courtesy of Sherine Elsawa, Northern Illinois University.

• Reactivity: Hu, Mu, Ca
• Applications: WB, ChIP, ELISA, Flow, Flow-IC, IB, IHC, IHC-P, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GLI-1 Antibody Summary

• Immunogen: A synthetic peptide made toward the C-terminus portion of the human GLI-1 protein (between residues 800-850) [Uniprot: P08151]
• Localization: Cytoplasmic and Nuclear
• Specificity: No reaction occurs with human or mouse Gli2 or Gli3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • ELISA 1:20000-1:100000
  • Flow Cytometry
  • Immunoblotting
  • Immunohistochemistry 1:600
  • Immunohistochemistry-Paraffin 1:600
  • Flow (Intracellular) 1-2ug/million cells
  • Knockdown Validated
• Application Notes: Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Flow was reported in a customer review. IHC-P reported from a verified customer review. Use in immunoblotting reported in scientific literature (PMID: 28167297). Use in FLOW reported in scientific literature (PMID: 26238488).
• Control:

  GLI-1 Overexpression Lysate

Reactivity Notes

Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GLI-1 Antibody

• GLI family zinc finger 1
• GLI1
• GLI-1
• glioma-associated oncogene family zinc finger 1
• glioma-associated oncogene homolog 1 (zinc finger protein)
• Glioma-associated oncogene
• GLIzinc finger protein GLI1
• Oncogene GLI

Background

Gli1 is a transcription factor involved in development, patterning and the regulation of cell fate-determination. A down-stream effector of the Hedgehog (HH) signaling pathway, Gli proteins work with other transcriptional activators to up-regulate the expression of Gli-response genes. In addition to its role in embryogenesis, the aberrant activation of the HH/Gli pathway has been identified in numerous forms of cancer, implicating the Gli family of proteins as potential therapeutic targets. In the absence of a stimulus, Gli1 is anchored to the cytoplasm via Cos-2, which is then released and translocates to the nucleus upon activation of the HH pathway. In the nucleus, Gli1 binds DNA via a highly conserved C2-H2 zinc finger domain and acts exclusively as an activator increasing transcription of GLI1, PTCH1, PTCH2, HHIP1, MYCN, CCND1, CCND2, BCL2, CFLAR, FOXF1, FOXL1, PRDM1 (BLIMP1), JAG2, GREM1, and Follistatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GLI-1 Antibody (NB600-600)(33)

Publications using NB600-600

• Singh SK, Gordetsky JB, Bae S et al. Selective Targeting of the Hedgehog Signaling Pathway by PBM Nanoparticles in Docetaxel-Resistant Prostate Cancer Cells Aug 27 2020 [PMID: 32867229] (IHC, ICC/IF, Flow, Human)
• Bacelar Sacramento de AraUjo T, de Oliveira Siquara da Rocha L, Torres Andion Vidal M et al. GANT61 Reduces Hedgehog Molecule (GLI1) Expression and Promotes Apoptosis in Metastatic Oral Squamous Cell Carcinoma Cells International journal of molecular sciences Aug 24 2020 [PMID: 32846867] (ICC/IF, WB, Human)
• Lin YL, Lin YW, Nhieu J et al. Sonic Hedgehog-Gli1 Signaling and Cellular Retinoic Acid Binding Protein 1 Gene Regulation in Motor Neuron Differentiation and Diseases Int J Mol Sci Jun 9 2020 [PMID: 32527063] (ChIP, Mouse)
• Safgren SL, Olson RLO, Vrabel AM et al. The transcription factor GLI1 cooperates with the chromatin remodeler SMARCA2 to regulate chromatin accessibility at distal DNA regulatory elements J. Biol. Chem. May 6 2020 [PMID: 32376693]
• Oak ASW, Bocheva G, Kim TK et al. Noncalcemic Vitamin D Hydroxyderivatives Inhibit Human Oral Squamous Cell Carcinoma and Down-regulate Hedgehog and WNT/beta-Catenin Pathways Anticancer Res. May 1 2020 [PMID: 32366390] (ICC/IF, Human)
• Chen PY, Qin L, Li G et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms Cell Stem Cell Apr 2 2020 [PMID: 32243809] (Mouse)
• Petty AJ, Li A, Wang X et al. Hedgehog signaling promotes tumor-associated macrophage polarization to suppress intratumoral CD8+ T cell recruitment J. Clin. Invest. 2019 Oct 22 [PMID: 31638600] (ChIP, Mouse)
• Mazzone A, Gibbons SJ, Eisenman ST et al. Direct repression of anoctamin 1 ( ANO1) gene transcription by Gli proteins FASEB J. Feb 25 2019 [PMID: 30802137] (ChIP, Human)
• Wang K, Chen D, Qian Z et al. Hedgehog/Gli1 signaling pathway regulates MGMT expression and chemoresistance to temozolomide in human glioblastoma. Cancer Cell Int. 2017 Dec 04 [PMID: 29225516] (ChIP, Human)
• Tanno B, Leonardi S, Babini G et al. Nanog-driven cell-reprogramming and self-renewal maintenance in Ptch1 +/- granule cell precursors after radiation injury Sci Rep. 2017 Oct 27 [PMID: 29079783] (WB, Mouse)
• Wei R, Lv M, Li F et al. Human CAFs promote lymphangiogenesis in ovarian cancer via the Hh-VEGF-C signaling axis. Oncotarget. 2017 Sep 15 [PMID: 28978035] (IHC-P, Mouse)
• Schneider RK, Mullally A, Dugourd A et al. Gli1(+) Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target. Cell Stem Cell. Apr 13 2017 [PMID: 28457748] (Mouse)
• Xu T, Zhang H, Park SS et al. Loss of Pin1 Suppresses Hedgehog-Driven Medulloblastoma Tumorigenesis. Neoplasia. 2017 Feb 03 [PMID: 28167297]
• Puri P Bushnell D Schaefer CM, Bates CM. Six2creFrs2a knockout mice are a novel model of renal cystogenesis. Sci Rep. Nov 17 2016 [PMID: 27853247] (WB, Mouse)
• Wu M, Ingram L, Tolosa EJ et al. Gli transcription factors mediate the oncogenic transformation of prostate basal cells induced by a Kras-androgen receptor axis. J. Biol. Chem. Oct 19 2016 [PMID: 27760825] (ChIP, Mouse)
• Vidal MT, Lourenco SV, Soares FA et al. The sonic hedgehog signaling pathway contributes to the development of salivary gland neoplasms regardless of perineural infiltration. Tumour Biol. 2016 Jan 21 [PMID: 26790448] (IHC-P, Human)
• Valverde LF, Pereira TA, Dias RB et al. Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation. Tumour Biol. 2016 Jan 15 [PMID: 26768620] (IHC-P, Human)
• Comba A, Almada LL, Tolosa EJ et al. Nuclear factor of activated T cells-dependent downregulation of the transcription factor GLI1 underlies the growth inhibitory properties of arachidonic acid. J. Biol. Chem. 2015 Nov 24 [PMID: 26601952] (ChIP, Human)
• Yeddula N, Xia Y, Ke E et al. Screening for tumor suppressors: Loss of ephrin receptor A2 cooperates with oncogenic KRas in promoting lung adenocarcinoma. Proc. Natl. Acad. Sci. U.S.A. 2015 Nov 24 [PMID: 26542681] (IHC-P, Mouse)
• Jackson DA, Smith TD, Amarsaikhan N et al. Modulation of the IL-6 Receptor alpha Underlies GLI2-Mediated Regulation of Ig Secretion in Waldenstrom Macroglobulinemia Cells. J Immunol 2015 Sep 15 [PMID: 26238488]
• Mazumdar T, Sandhu R, Qadan M et al. Hedgehog Signaling Regulates Telomerase Reverse Transcriptase in Human Cancer Cells. PLoS One. 2013 Sep 25 [PMID: 24086482] (ChIP, WB, Human)
• Karlou M, Lu JF, Wu G et al. Hedgehog signaling inhibition by the small molecule smoothened inhibitor GDC-0449 in the bone forming prostate cancer xenograft MDA PCa 118b Prostate 2012 Nov [PMID: 22457212] (IHC, Human)
• Mazumdar T, Devecchio J, Agyeman A et al. Blocking Hedgehog survival signaling at the level of the GLI genes induces DNA damage and extensive cell death in human colon carcinoma cells. Cancer Res. 2011 Jul 11. [PMID: 21747117]
• Tzelepi V, Karlou M, Wen S et al. Expression of hedgehog pathway components in prostate carcinoma microenvironment: shifting the balance towards autocrine signalling. Histopathology;58(7):1037-1047. 2011 Jun. [PMID: 21707705] (IHC, Human)
• Mazumdar T, DeVecchio J, Shi T et al. Hedgehog signaling drives cellular survival in human colon carcinoma cells Cancer Res 2011 Feb [PMID: 21135115] (WB, Human)
• Breunig JJ, Sarkisian MR, Arellano JI et al. Primary cilia regulate hippocampal neurogenesis by mediating sonic hedgehog signaling. PNAS;105(35):13127-13132. 2008 [PMID: 15170664]
• Niemann C et al. Indian hedgehog beta-catenin signaling: role in the sebaceous lineage of normal neoplastic mammalian epidermis. Proc. Natl. Acad. Sci. USA 100 Suppl 1:11873-80. 2003 [PMID: 12917489] (WB, ChIP, Human)
• Dos Santos JN. Estudo imunoistoquimico de componentes da via Sonic Hedgehog, STAT3 e MCM3 em tumores de glandula salivar. Thesis. 2015 (IHC-P, Human)
• Dias RB. Estudo do perfil de expressao de componentes da via de sinalizacao sonic hedgehog em displasia epitelial oral [Study of the expression of components of sonic hedgehog signaling pathway in oral epithelial dysplasia profile. Thesis: http://arca.icict.fiocruz.br/handle/icict/7483]. Thesis (IHC-P, Human)
• Jiang H, Li F, He C et al. Expression of Gli1 and Wnt2B correlates with progression and clinical outcome of pancreatic cancer. Int J Clin Exp Pathol. 2014 Aug 14 [PMID: 25120849] (IHC-P, Human)
• Nye MD, Almada LL, Fernandez-Barrena MG et al. The transcription factor GLI1 interacts with SMAD proteins to modulate TGFbeta-induced gene expression in a PCAF-dependent manner. J. Biol. Chem. 2014 Apr 16 [PMID: 24739390] (WB, ChIP, Human)
• Huang L, Walter V, Hayes DN. Hedgehog-GLI Signaling Inhibition Suppresses Tumor Growth in Squamous Lung Cancer. Clin. Cancer Res. 2014 Mar 5 [PMID: 24423612] (WB, Human)
• Regard JB, Malhotra D, Gvozdenovic-Jeremic J et al. Activation of Hedgehog signaling by loss of GNAS causes heterotopic ossification. Nat Med. 2013 Sep 29 [PMID: 24076664] (WB, Mouse)

Review for GLI-1 Antibody (NB600-600) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: Anonymous IHC-P Canine 06/12/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Skin (hair follicle)
• Species: Canine
• Lot: C-2

Comments

• Comments: Antibody dilution = 1:600. 3, 3'-diaminobenzidine (DAB) chromogen and counterstaining with Mayer’s hematoxylin.

Bioinformatics

• Gene Symbol: GLI1
• Entrez:
  • 2735(Human)
  • 14632(Mouse)
• Uniprot:
  • P08151()