GLI-1 Antibody

Images

 
Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Staining in canine hair follicle. Image from a verified customer review.
Flow (Intracellular): GLI-1 Antibody [NB600-600] - Staining for GLI-1 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-1 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a ...read more
Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Analysis of a FFPE mouse brain section using GLI-1 antibody at 1:600 dilution.

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, ChIP, ELISA, Flow, Flow-IC, IB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

GLI-1 Antibody Summary

Immunogen
A synthetic peptide made toward the C-terminus portion of the human GLI-1 protein (between residues 800-850) [Uniprot: P08151]
Localization
Cytoplasmic and Nuclear
Specificity
No reaction occurs with human or mouse Gli2 or Gli3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • ELISA 1:20000-1:100000
  • Flow Cytometry
  • Immunoblotting
  • Immunohistochemistry 1:600
  • Immunohistochemistry-Paraffin 1:600
  • Flow (Intracellular) 1-2ug/million cells
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Flow was reported in a customer review. IHC-P reported from a verified customer review. Use in immunoblotting reported in scientific literature (PMID: 28167297). Use in FLOW reported in scientific literature (PMID: 26238488).
Control
GLI-1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB600-600 in the following applications:

Publications
Read Publications using
NB600-600 in the following applications:

  • 8 publications
  • IHC
    2 publications
  • 7 publications
  • WB
    8 publications

Reactivity Notes

Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GLI-1 Antibody

  • GLI family zinc finger 1
  • GLI1
  • GLI-1
  • glioma-associated oncogene family zinc finger 1
  • glioma-associated oncogene homolog 1 (zinc finger protein)
  • Glioma-associated oncogene
  • GLIzinc finger protein GLI1
  • Oncogene GLI

Background

Gli1 is a transcription factor involved in development, patterning and the regulation of cell fate-determination. A down-stream effector of the Hedgehog (HH) signaling pathway, Gli proteins work with other transcriptional activators to up-regulate the expression of Gli-response genes. In addition to its role in embryogenesis, the aberrant activation of the HH/Gli pathway has been identified in numerous forms of cancer, implicating the Gli family of proteins as potential therapeutic targets. In the absence of a stimulus, Gli1 is anchored to the cytoplasm via Cos-2, which is then released and translocates to the nucleus upon activation of the HH pathway. In the nucleus, Gli1 binds DNA via a highly conserved C2-H2 zinc finger domain and acts exclusively as an activator increasing transcription of GLI1, PTCH1, PTCH2, HHIP1, MYCN, CCND1, CCND2, BCL2, CFLAR, FOXF1, FOXL1, PRDM1 (BLIMP1), JAG2, GREM1, and Follistatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-600
Species: Hu, Mu, Ca
Applications: WB, ChIP, ELISA, Flow, Flow-IC, IB, IHC, IHC-P

Publications for GLI-1 Antibody (NB600-600)(27)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ChIP, IHC, IHC-P, WB.


Filter By Application
ChIP
(8)
IHC
(2)
IHC-P
(7)
WB
(8)
All Applications
Filter By Species
Human
(15)
Mouse
(8)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using NB600-600 Applications Species
Petty AJ, Li A, Wang X et al. Hedgehog signaling promotes tumor-associated macrophage polarization to suppress intratumoral CD8+ T cell recruitment J. Clin. Invest. 2019 Oct 22 [PMID: 31638600] (ChIP, Mouse) ChIP Mouse
Mazzone A, Gibbons SJ, Eisenman ST et al. Direct repression of anoctamin 1 ( ANO1) gene transcription by Gli proteins FASEB J. Feb 25 2019 [PMID: 30802137] (ChIP, Human) ChIP Human
Wang K, Chen D, Qian Z et al. Hedgehog/Gli1 signaling pathway regulates MGMT expression and chemoresistance to temozolomide in human glioblastoma. Cancer Cell Int. 2017 Dec 04 [PMID: 29225516] (ChIP, Human) ChIP Human
Tanno B, Leonardi S, Babini G et al. Nanog-driven cell-reprogramming and self-renewal maintenance in Ptch1 +/- granule cell precursors after radiation injury Sci Rep. 2017 Oct 27 [PMID: 29079783] (WB, Mouse) WB Mouse
Wei R, Lv M, Li F et al. Human CAFs promote lymphangiogenesis in ovarian cancer via the Hh-VEGF-C signaling axis. Oncotarget. 2017 Sep 15 [PMID: 28978035] (IHC-P, Mouse) IHC-P Mouse
Schneider RK, Mullally A, Dugourd A et al. Gli1(+) Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target. Cell Stem Cell. Apr 13 2017 [PMID: 28457748] (Mouse) Mouse
Xu T, Zhang H, Park SS et al. Loss of Pin1 Suppresses Hedgehog-Driven Medulloblastoma Tumorigenesis. Neoplasia. 2017 Feb 03 [PMID: 28167297]
Puri P Bushnell D Schaefer CM, Bates CM. Six2creFrs2a knockout mice are a novel model of renal cystogenesis. Sci Rep. Nov 17 2016 [PMID: 27853247] (WB, Mouse) WB Mouse
Wu M, Ingram L, Tolosa EJ et al. Gli transcription factors mediate the oncogenic transformation of prostate basal cells induced by a Kras-androgen receptor axis. J. Biol. Chem. Oct 19 2016 [PMID: 27760825] (ChIP, Mouse) ChIP Mouse
Vidal MT, Lourenco SV, Soares FA et al. The sonic hedgehog signaling pathway contributes to the development of salivary gland neoplasms regardless of perineural infiltration. Tumour Biol. 2016 Jan 21 [PMID: 26790448] (IHC-P, Human) IHC-P Human
Show All 27 Publications.

Review for GLI-1 Antibody (NB600-600) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Canine.

Reviews using NB600-600:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Canine
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GLI-1 NB600-600
Enlarge
reviewed by:
Anonymous
IHC-P Canine 06/12/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSkin (hair follicle)
SpeciesCanine
LotC-2

Comments

CommentsAntibody dilution = 1:600. 3, 3'-diaminobenzidine (DAB) chromogen and counterstaining with Mayer’s hematoxylin.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for GLI-1 Antibody (NB600-600) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-600AF350
Alexa Fluor 405 NB600-600AF405
Alexa Fluor 488 NB600-600AF488
Alexa Fluor 532 NB600-600AF532
Alexa Fluor 594 NB600-600AF594
Alexa Fluor 647 NB600-600AF647
Alexa Fluor 700 NB600-600AF700
Alexa Fluor 750 NB600-600AF750
Biotin NB600-600B
DyLight 350 NB600-600UV
DyLight 405 NB600-600V
DyLight 488 NB600-600G
DyLight 550 NB600-600R
DyLight 594 NB600-600DL594
DyLight 650 NB600-600C
DyLight 680 NB600-600FR
DyLight 755 NB600-600IR
FITC NB600-600F
HRP NB600-600H
Janelia Fluor 549 NB600-600JF549
Janelia Fluor 646 NB600-600JF646

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Blogs on GLI-1

There are no specific blogs for GLI-1, but you can read our latest blog posts.
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Contact Information

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Calculators

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
06/12/2019
Application: IHC-P
Species: Canine

Bioinformatics

Gene Symbol GLI1
Entrez
Uniprot
COVID-19 update