GLI-1 Antibody - BSA Free

Images

 
Genetic Strategies: Knockdown Validated: GLI-1 Antibody [NB600-600] - Inhibition of Hh signaling in CAFs blocks its effects on lymphangiogenesis in vivo and in vitro. (E) IHC staining for Ki-67, -SMA, Gli-1 and ...read more
Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Analysis of a FFPE mouse brain section using GLI-1 antibody at 1:600 dilution.
Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Staining in canine hair follicle. Image from a verified customer review.
Flow (Intracellular): GLI-1 Antibody [NB600-600] - Staining for GLI-1 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-1 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a ...read more

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, ChIP, ELISA, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

GLI-1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made toward the C-terminus portion of the human GLI-1 protein (between residues 800-850) [Uniprot: P08151]
Localization
Cytoplasmic and Nuclear
Specificity
No reaction occurs with human or mouse Gli2 or Gli3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10-1:500
  • ELISA 1:20000-1:100000
  • Flow (Intracellular) 1-2ug/million cells
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:600
  • Immunohistochemistry-Paraffin 1:600
  • Knockdown Validated
  • Western Blot 1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID:35226587) Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Flow was reported in a customer review. IHC-P reported from a verified customer review. Use in immunoblotting reported in scientific literature (PMID: 28167297). Use in FLOW reported in scientific literature (PMID: 26238488)..
Control
GLI-1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB600-600 in the following applications:

Publications
Read Publications using
NB600-600 in the following applications:

  • 10 publications
  • 1 publication
  • 5 publications
  • IHC
    4 publications
  • 8 publications
  • WB
    11 publications

Reactivity Notes

Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GLI-1 Antibody - BSA Free

  • GLI family zinc finger 1
  • GLI1
  • GLI-1
  • glioma-associated oncogene family zinc finger 1
  • glioma-associated oncogene homolog 1 (zinc finger protein)
  • Glioma-associated oncogene
  • GLIzinc finger protein GLI1
  • Oncogene GLI

Background

Gli1 is a transcription factor involved in development, patterning and the regulation of cell fate-determination. A down-stream effector of the Hedgehog (HH) signaling pathway, Gli proteins work with other transcriptional activators to up-regulate the expression of Gli-response genes. In addition to its role in embryogenesis, the aberrant activation of the HH/Gli pathway has been identified in numerous forms of cancer, implicating the Gli family of proteins as potential therapeutic targets. In the absence of a stimulus, Gli1 is anchored to the cytoplasm via Cos-2, which is then released and translocates to the nucleus upon activation of the HH pathway. In the nucleus, Gli1 binds DNA via a highly conserved C2-H2 zinc finger domain and acts exclusively as an activator increasing transcription of GLI1, PTCH1, PTCH2, HHIP1, MYCN, CCND1, CCND2, BCL2, CFLAR, FOXF1, FOXL1, PRDM1 (BLIMP1), JAG2, GREM1, and Follistatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-600
Species: Hu, Mu, Ca
Applications: WB, ChIP, ELISA, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-P, KD

Publications for GLI-1 Antibody (NB600-600)(43)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 6 applications: ChIP, Flow, ICC/IF, IHC, IHC-P, WB.


Filter By Application
ChIP
(10)
Flow
(1)
ICC/IF
(5)
IHC
(4)
IHC-P
(8)
WB
(11)
All Applications
Filter By Species
Human
(21)
Mouse
(14)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using NB600-600 Applications Species
Dos Santos JN. Estudo imunoistoquimico de componentes da via Sonic Hedgehog, STAT3 e MCM3 em tumores de glandula salivar. Thesis. 2015 (IHC-P, Human) IHC-P Human
Gonzalez-Rodriguez P, Cheray M, Keane L et al. ULK3-dependent activation of GLI1 promotes DNMT3A expression upon autophagy induction Autophagy Feb 28 2022 [PMID: 35226587] (WB, ICC/IF, Human) WB, ICC/IF Human
Reyes NicolAs V, Allaire J, Alfonso A Et al. Altered Mucus Barrier Integrity and Increased Susceptibility to Colitis in Mice upon Loss of Telocyte Bone Morphogenetic Protein Signalling Cells Oct 29 2021 [PMID: 34831177] (ICC/IF, Mouse) ICC/IF Mouse
Dalton GD, Oh SH, Tang L et al. Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice iScience Sep 24 2021 [PMID: 34568800] (WB, Mouse) WB Mouse
Olson AW, Le V, Wang J Et al. Stromal androgen and hedgehog signaling regulates stem cell niches in pubertal prostate development Development (Cambridge, England) Aug 24 2021 [PMID: 34427305]
Shi Y, Liao X, Long JY Et al. Gli1+ progenitors mediate bone anabolic function of teriparatide via Hh and Igf signaling Cell reports Aug 17 2021 [PMID: 34407400] (Mouse) Mouse
Ichikawa D, Yamashita K, Okuno Y et al. Integrated diagnosis based on transcriptome analysis in suspected pediatric sarcomas NPJ genomic medicine Jun 15 2021 [PMID: 34131151] (IHC-P, Human) IHC-P Human
Wei Y, Sun H, Gui T et al. The critical role of Hedgehog-responsive mesenchymal progenitors in meniscus development and injury repair eLife Jun 4 2021 [PMID: 34085927] (IHC, Mouse, Porcine, Human) IHC Mouse, Porcine, Human
Boukhalfa A, Roccio F, Dupont N et al. The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium Cell reports Apr 27 2021 [PMID: 33910006]
Wang W, Yan T, Guo W et al. Constitutive GLI1 expression in chondrosarcoma is regulated by major vault protein via mTOR/S6K1 signaling cascade Cell death and differentiation Feb 26 2021 [PMID: 33637972] (ChIP) ChIP
Show All 43 Publications.

Review for GLI-1 Antibody (NB600-600) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Canine.

Reviews using NB600-600:
Filter by Applications
Immunohistochemistry-Paraffin
(1)
All Applications
Filter by Species
Canine
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GLI-1 NB600-600
Enlarge
5
reviewed by:
Mun Keong Kok
Immunohistochemistry-Paraffin Canine 06/12/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSkin (hair follicle)
SpeciesCanine
LotC-2

Comments

CommentsAntibody dilution = 1:600. 3, 3'-diaminobenzidine (DAB) chromogen and counterstaining with Mayer’s hematoxylin.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for GLI-1 Antibody (NB600-600) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Contact Information

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Mun Keong Kok
06/12/2019
Application: Immunohistochemistry-Paraffin
Species: Canine

Bioinformatics

Gene Symbol GLI1
Entrez
Uniprot