GLI-1 Antibody - BSA Free

Immunohistochemistry: GLI-1 Antibody - BSA Free [NB600-600] - GLI-1 Antibody [NB600-600] - Inhibition of Hh signaling in CAFs blocks its effects on lymphangiogenesis in vivo and in vitro. (E) IHC staining for Ki-67, -SMA, Gli-1 and LYVE1 in tumour sections from mice in the two groups. Human CAFs promote lymphangiogenesis in ovarian cancer via the Hh-VEGF-C signaling axis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31012844/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Analysis of a FFPE mouse brain section using GLI-1 antibody at 1:600 dilution.

Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Staining in canine hair follicle. Image from a verified customer review.

Flow (Intracellular): GLI-1 Antibody [NB600-600] - Staining for GLI-1 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-1 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a negative control. Image courtesy of Sherine Elsawa, Northern Illinois University.

Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600] - Paracrine signaling of tumour-derived SHH in CAFs promotes lymphangiogenesis in vivo(A) Growth properties of subcutaneous tumours in mice derived from Caov3 SHH-OE or Caov3 NC cells alone or co-injected with CAFs. Kaplan-Meier plot of time to tumour progression, as evidenced by survival rates over time (n=5). (B) Bright field images of subcutaneous tumours from each group (n=5) 20 days after tumour implantation. (C) Graphical representation of the weights of subcutaneous tumours from each group 20 days after tumour implantation. (D) Immunohistochemical staining for Ki-67, alpha -SMA, Gli-1 & LYVE1 in tumour sections from mice in each group. (E) Graphical representation of the number of LYVE1+ vessels counted from IHC images in panel D. (*P<0.05, **P<0.01, ***P<0.001). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.18621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600] - Inhibition of Hh signaling in CAFs blocks its effects on lymphangiogenesis in vivo & in vitro(A) Representative images & statistical analyses of migration of LECs in a co-culture invasion system with CAFs stably transfected with shNC or Gli-shRNA lentivirus in the presence of rSHH (1 μg/ml). (B) Representative images & statistical analyses of capillary tube formation of LECs treated with supernatants from cultures of CAFs stably transfected with shNC or Gli-shRNA lentivirus in the presence of rSHH (1 μg/ml). (C) Bright field images & graphical representation of the weights of tumours of subcutaneous tumours from mice (n=5) bearing Caov3 SHH-OE cells co-injected with CAFs stably transfected with shNC or Gli-shRNA lentivirus 20 days after tumour implantation. (D) Growth properties of subcutaneous tumours in mice derived from Caov3 SHH-OE cells co-injected with CAFs stably transfected with shNC or Gli-shRNA lentivirus. Kaplan-Meier plot of time to tumour progression, as evidenced by survival rates over time. (E) IHC staining for Ki-67, alpha -SMA, Gli-1 & LYVE1 in tumour sections from mice in the two groups. (F) Graphical representation of the number of LYVE1+ vessels counted from IHC images in panel E. (**P<0.01, ***P<0.001). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.18621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Ca
• Applications: WB, ELISA, Flow, ICC/IF, IHC, WB, ChIP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GLI-1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made toward the C-terminus portion of the human GLI-1 protein (between residues 800-850) [Uniprot: P08151]
• Localization: Cytoplasmic and Nuclear
• Specificity: No reaction occurs with human or mouse Gli2 or Gli3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • ELISA 1:500 - 1:2000
  • Flow (Intracellular) 1-2 ug/million cells
  • Immunoblotting 1 - 2 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Knockdown Validated
  • Western Blot 1 - 2 ug/ml
• Application Notes: Use in ICC/IF reported in scientific literature (PMID:35226587) Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Flow was reported in a customer review. IHC-P reported from a verified customer review. Use in immunoblotting reported in scientific literature (PMID: 28167297). Use in FLOW reported in scientific literature (PMID: 26238488)..
• Control:

  GLI-1 Overexpression Lysate

Reactivity Notes

Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GLI-1 Antibody - BSA Free

• GLI family zinc finger 1
• GLI1
• GLI-1
• glioma-associated oncogene family zinc finger 1
• glioma-associated oncogene homolog 1 (zinc finger protein)
• Glioma-associated oncogene
• GLIzinc finger protein GLI1
• Oncogene GLI

Background

Gli1 is a transcription factor involved in development, patterning and the regulation of cell fate-determination. A down-stream effector of the Hedgehog (HH) signaling pathway, Gli proteins work with other transcriptional activators to up-regulate the expression of Gli-response genes. In addition to its role in embryogenesis, the aberrant activation of the HH/Gli pathway has been identified in numerous forms of cancer, implicating the Gli family of proteins as potential therapeutic targets. In the absence of a stimulus, Gli1 is anchored to the cytoplasm via Cos-2, which is then released and translocates to the nucleus upon activation of the HH pathway. In the nucleus, Gli1 binds DNA via a highly conserved C2-H2 zinc finger domain and acts exclusively as an activator increasing transcription of GLI1, PTCH1, PTCH2, HHIP1, MYCN, CCND1, CCND2, BCL2, CFLAR, FOXF1, FOXL1, PRDM1 (BLIMP1), JAG2, GREM1, and Follistatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GLI-1 Antibody (NB600-600)(57)

Publications using NB600-600

• Bo Ram Kim, Dae Yeong Kim, Na Ly Tran, Bu Gyeom Kim, Sun Il Lee, Sang Hee Kang, Byung Yook Min, Wooyoung Hur, Sang Cheul Oh Daunorubicin induces GLI1-dependent apoptosis in colorectal cancer cell lines International Journal of Oncology 2024-06-01 [PMID: 38757343]
• Matko Leović, Antonija Jakovčević, Ivan Mumlek, Irena Zagorac, Maja Sabol, Dinko Leović, Marko Tarle A Pilot Immunohistochemical Study Identifies Hedgehog Pathway Expression in Sinonasal Adenocarcinoma International Journal of Molecular Sciences 2024-04-24 [PMID: 38731849]
• Hong Zhou, Qun Zhang, Chenyang Liu, Jiahao Fan, Wen Huang, Nan Li, Mingxia Yang, Hong Wang, Weiping Xie, Hui Kong NLRP3 inflammasome mediates abnormal epithelial regeneration and distal lung remodeling in silica-induced lung fibrosis International Journal of Molecular Medicine 2024-03-01 [PMID: 38240085]
• Orzechowska-Licari EJ, Bialkowska AB, Yang VW Sonic Hedgehog and WNT signaling regulate a positive feedback loop between intestinal epithelial and stromal cells to promote epithelial regeneration Cellular and molecular gastroenterology and hepatology 2023-07-20 [PMID: 37481204] (WB, Mouse)
  
  Details:
  1:400 WB dilution
• Vera RE, Lamberti MJ, Almada LL et al. GLI1 interaction with p300 modulates SDF1 expression in cancer-associated fibroblasts to promote pancreatic cancer cells migration The Biochemical journal 2023-02-15 [PMID: 36734208]
• Dias RB. Estudo do perfil de expressao de componentes da via de sinalizacao sonic hedgehog em displasia epitelial oral [Study of the expression of components of sonic hedgehog signaling pathway in oral epithelial dysplasia profile. Thesis: http://arca.icict.fiocruz.br/handle/icict/7483]. Thesis (IHC-P, Human)
• Hasselluhn, MC;Decker-Farrell, AR;Vlahos, L;Thomas, DH;Curiel-Garcia, A;Maurer, HC;Wasko, UN;Tomassoni, L;Sastra, SA;Palermo, CF;Dalton, TC;Ma, A;Li, F;Tolosa, EJ;Hibshoosh, H;Fernandez-Zapico, ME;Muir, A;Califano, A;Olive, KP; Tumor Explants Elucidate a Cascade of Paracrine SHH, WNT, and VEGF Signals Driving Pancreatic Cancer Angiosuppression Cancer discovery 2023-11-15 [PMID: 37966260]
• Koo H, Lee S, Kim WH Usability of serum hedgehog signalling proteins as biomarkers in canine mammary carcinomas BMC veterinary research 2023-11-06 [PMID: 37932728] (WB, Canine)
• Wang M, Huang W FOXS1 promotes prostate cancer progression through the Hedgehog/Gli1 pathway Biochemical pharmacology 2023-10-27 [PMID: 37890593]
• Almada LL, Barroso K, Sen S et al. GLI1, a novel target of the ER stress regulator p97/VCP, promotes ATF6f-mediated activation of XBP1 Biochimica et biophysica acta. Gene regulatory mechanisms 2023-02-24 [PMID: 36842643] (ChIP)
• Liu Y, Liu S, Song Z et al. GLI1 Deficiency Impairs the Tendon-Bone Healing after Anterior Cruciate Ligament Reconstruction: In Vivo Study Using Gli1-Transgenic Mice Journal of Clinical Medicine 2023-01-28 [PMID: 36769647] (Chemotaxis, Western Blot, Human)
• Yamakawa D, Tsuboi J, Kasahara K et al. Cilia-Mediated Insulin/Akt and ST2/JNK Signaling Pathways Regulate the Recovery of Muscle Injury Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022-11-14 [PMID: 36373718] (WB, Mouse)
  
  Details:
  Dilution used in WB 1:1000
• Angelini A, Ortiz-Urbina J, Trial J, Reddy AK et Al. Sex-specific phenotypes in the aging mouse heart and consequences for chronic fibrosis Am J Physiol Heart Circ Physiol 2022-06-17 [PMID: 35714177]
• Gonzalez-Rodriguez P, Cheray M, Keane L et al. ULK3-dependent activation of GLI1 promotes DNMT3A expression upon autophagy induction Autophagy 2022-02-28 [PMID: 35226587] (WB, ICC/IF, Human)
• Reyes NicolAs V, Allaire J, Alfonso A Et al. Altered Mucus Barrier Integrity and Increased Susceptibility to Colitis in Mice upon Loss of Telocyte Bone Morphogenetic Protein Signalling Cells 2021-10-29 [PMID: 34831177] (ICC/IF, Mouse)
• Dalton GD, Oh SH, Tang L et al. Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice iScience 2021-09-24 [PMID: 34568800] (WB, Mouse)
• Olson AW, Le V, Wang J Et al. Stromal androgen and hedgehog signaling regulates stem cell niches in pubertal prostate development Development (Cambridge, England) 2021-08-24 [PMID: 34427305]
• Shi Y, Liao X, Long JY Et al. Gli1+ progenitors mediate bone anabolic function of teriparatide via Hh and Igf signaling Cell reports 2021-08-17 [PMID: 34407400] (Mouse)
• Ichikawa D, Yamashita K, Okuno Y et al. Integrated diagnosis based on transcriptome analysis in suspected pediatric sarcomas NPJ genomic medicine 2021-06-15 [PMID: 34131151] (IHC-P, Human)
• Wei Y, Sun H, Gui T et al. The critical role of Hedgehog-responsive mesenchymal progenitors in meniscus development and injury repair eLife 2021-06-04 [PMID: 34085927] (IF/IHC, Porcine, Mouse, Human)
• Boukhalfa A, Roccio F, Dupont N et al. The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium Cell reports 2021-04-27 [PMID: 33910006]
• Wang W, Yan T, Guo W et al. Constitutive GLI1 expression in chondrosarcoma is regulated by major vault protein via mTOR/S6K1 signaling cascade Cell death and differentiation 2021-02-26 [PMID: 33637972] (Chemotaxis)
• Tanigawa S, Fujita M, Moyama C, et al. Inhibition of Gli2 suppresses tumorigenicity in glioblastoma stem cells derived from a de novo murine brain cancer model Cancer gene therapy 2021-01-07 [PMID: 33414520]
• Qian Wang, Shengnan Jia, Ding Wang, Xuyang Chen, Dhan V Kalvakolanu, Hongwu Zheng, Xiaodong Wei, Naiyan Wen, Hang Liang, Baofeng Guo, Ling Zhang A Combination of BRD4 and HDAC3 Inhibitors Synergistically Suppresses Glioma Stem Cell Growth by Blocking GLI1/IL6/STAT3 Signaling Axis. Molecular cancer therapeutics 2021-08-12 [PMID: 32999044]
• Singh SK, Gordetsky JB, Bae S et al. Selective Targeting of the Hedgehog Signaling Pathway by PBM Nanoparticles in Docetaxel-Resistant Prostate Cancer Cells 2020-08-27 [PMID: 32867229] (FLOW, IF/IHC, ICC/IF, Human)
• Bacelar Sacramento de AraUjo T, de Oliveira Siquara da Rocha L, Torres Andion Vidal M et al. GANT61 Reduces Hedgehog Molecule (GLI1) Expression and Promotes Apoptosis in Metastatic Oral Squamous Cell Carcinoma Cells International journal of molecular sciences 2020-08-24 [PMID: 32846867] (WB, ICC/IF, Human)
• Evelyn Susanto, Ana Marin Navarro, Leilei Zhou, Anders Sundström, Niek van Bree, Marina Stantic, Mohsen Moslem, Jignesh Tailor, Jonne Rietdijk, Veronica Zubillaga, Jens-Martin Hübner, Holger Weishaupt, Johanna Wolfsberger, Irina Alafuzoff, Ann Nordgren, Thierry Magnaldo, Peter Siesjö, John Inge Johnsen, Marcel Kool, Kristiina Tammimies, Anna Darabi, Fredrik J. Swartling, Anna Falk, Margareta Wilhelm Modeling SHH-driven medulloblastoma with patient iPS cell-derived neural stem cells Proceedings of the National Academy of Sciences of the United States of America 2020-08-18 [PMID: 32747535]
• Lin YL, Lin YW, Nhieu J et al. Sonic Hedgehog-Gli1 Signaling and Cellular Retinoic Acid Binding Protein 1 Gene Regulation in Motor Neuron Differentiation and Diseases Int J Mol Sci 2020-06-09 [PMID: 32527063] (Chemotaxis, Mouse)
• Safgren SL, Olson RLO, Vrabel AM et al. The transcription factor GLI1 cooperates with the chromatin remodeler SMARCA2 to regulate chromatin accessibility at distal DNA regulatory elements J. Biol. Chem. 2020-05-06 [PMID: 32376693]
• Oak ASW, Bocheva G, Kim TK et al. Noncalcemic Vitamin D Hydroxyderivatives Inhibit Human Oral Squamous Cell Carcinoma and Down-regulate Hedgehog and WNT/beta-Catenin Pathways Anticancer Res. 2020-05-01 [PMID: 32366390] (ICC/IF, Human)
• Chen PY, Qin L, Li G et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms Cell Stem Cell 2020-04-02 [PMID: 32243809] (Mouse)
• Petty AJ, Li A, Wang X et al. Hedgehog signaling promotes tumor-associated macrophage polarization to suppress intratumoral CD8+ T cell recruitment J. Clin. Invest. 2019-10-22 [PMID: 31638600] (Chemotaxis, Mouse)
• Mazzone A, Gibbons SJ, Eisenman ST et al. Direct repression of anoctamin 1 ( ANO1) gene transcription by Gli proteins FASEB J. 2019-02-25 [PMID: 30802137] (Chemotaxis, Human)
• Wang K, Chen D, Qian Z et al. Hedgehog/Gli1 signaling pathway regulates MGMT expression and chemoresistance to temozolomide in human glioblastoma. Cancer Cell Int. 2017-12-04 [PMID: 29225516] (Chemotaxis, Human)
• Tanno B, Leonardi S, Babini G et al. Nanog-driven cell-reprogramming and self-renewal maintenance in Ptch1 +/- granule cell precursors after radiation injury Sci Rep. 2017-10-27 [PMID: 29079783] (WB, Mouse)
• Wei R, Lv M, Li F et al. Human CAFs promote lymphangiogenesis in ovarian cancer via the Hh-VEGF-C signaling axis. Oncotarget. 2017-09-15 [PMID: 28978035] (IHC-P, Mouse)
• Schneider RK, Mullally A, Dugourd A et al. Gli1(+) Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target. Cell Stem Cell. 2017-04-13 [PMID: 28457748] (Mouse)
• Xu T, Zhang H, Park SS et al. Loss of Pin1 Suppresses Hedgehog-Driven Medulloblastoma Tumorigenesis. Neoplasia. 2017-02-03 [PMID: 28167297]
• Puri P Bushnell D Schaefer CM, Bates CM. Six2creFrs2a knockout mice are a novel model of renal cystogenesis. Sci Rep. 2016-11-17 [PMID: 27853247] (WB, Mouse)
• Wu M, Ingram L, Tolosa EJ et al. Gli transcription factors mediate the oncogenic transformation of prostate basal cells induced by a Kras-androgen receptor axis. J. Biol. Chem. 2016-10-19 [PMID: 27760825] (Chemotaxis, Mouse)
• Vidal MT, Lourenco SV, Soares FA et al. The sonic hedgehog signaling pathway contributes to the development of salivary gland neoplasms regardless of perineural infiltration. Tumour Biol. 2016-01-21 [PMID: 26790448] (IHC-P, Human)
• Valverde LF, Pereira TA, Dias RB et al. Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation. Tumour Biol. 2016-01-15 [PMID: 26768620] (IHC-P, Human)
• Comba A, Almada LL, Tolosa EJ et al. Nuclear factor of activated T cells-dependent downregulation of the transcription factor GLI1 underlies the growth inhibitory properties of arachidonic acid. J. Biol. Chem. 2015-11-24 [PMID: 26601952] (Chemotaxis, Human)
• Yeddula N, Xia Y, Ke E et al. Screening for tumor suppressors: Loss of ephrin receptor A2 cooperates with oncogenic KRas in promoting lung adenocarcinoma. Proc. Natl. Acad. Sci. U.S.A. 2015-11-24 [PMID: 26542681] (IHC-P, Mouse)
• Jackson DA, Smith TD, Amarsaikhan N et al. Modulation of the IL-6 Receptor alpha Underlies GLI2-Mediated Regulation of Ig Secretion in Waldenstrom Macroglobulinemia Cells. J Immunol 2015-09-15 [PMID: 26238488]
• Jiang H, Li F, He C et al. Expression of Gli1 and Wnt2B correlates with progression and clinical outcome of pancreatic cancer. Int J Clin Exp Pathol. 2014-08-14 [PMID: 25120849] (IHC-P, Human)
• Nye MD, Almada LL, Fernandez-Barrena MG et al. The transcription factor GLI1 interacts with SMAD proteins to modulate TGFbeta-induced gene expression in a PCAF-dependent manner. J. Biol. Chem. 2014-04-16 [PMID: 24739390] (Chemotaxis, WB, Human)
• Tzelepi V, Karlou M, Wen S et al. Expression of hedgehog pathway components in prostate carcinoma microenvironment: shifting the balance towards autocrine signalling. Histopathology;58(7):1037-1047. 2011-06-01 [PMID: 21707705] (IF/IHC, Human)
• Dos Santos JN. Estudo imunoistoquimico de componentes da via Sonic Hedgehog, STAT3 e MCM3 em tumores de glandula salivar. Thesis. 2015-01-01 (IHC-P, Human)
• Huang L, Walter V, Hayes DN. Hedgehog-GLI Signaling Inhibition Suppresses Tumor Growth in Squamous Lung Cancer. Clin. Cancer Res. 2014-03-05 [PMID: 24423612] (WB, Human)
• Mazumdar T, Sandhu R, Qadan M et al. Hedgehog Signaling Regulates Telomerase Reverse Transcriptase in Human Cancer Cells. PLoS One. 2013-09-25 [PMID: 24086482] (Chemotaxis, WB, Human)
• Regard JB, Malhotra D, Gvozdenovic-Jeremic J et al. Activation of Hedgehog signaling by loss of GNAS causes heterotopic ossification. Nat Med. 2013-09-29 [PMID: 24076664] (WB, Mouse)
• Karlou M, Lu JF, Wu G et al. Hedgehog signaling inhibition by the small molecule smoothened inhibitor GDC-0449 in the bone forming prostate cancer xenograft MDA PCa 118b Prostate 2012-11-01 [PMID: 22457212] (IF/IHC, Human)
• Mazumdar T, Devecchio J, Agyeman A et al. Blocking Hedgehog survival signaling at the level of the GLI genes induces DNA damage and extensive cell death in human colon carcinoma cells. Cancer Res. 2011-07-11 [PMID: 21747117]
• Mazumdar T, DeVecchio J, Shi T et al. Hedgehog signaling drives cellular survival in human colon carcinoma cells Cancer Res 2011-02-01 [PMID: 21135115] (WB, Human)
• Breunig JJ, Sarkisian MR, Arellano JI et al. Primary cilia regulate hippocampal neurogenesis by mediating sonic hedgehog signaling. PNAS;105(35):13127-13132. 2008-01-01 [PMID: 15170664]
• Niemann C et al. Indian hedgehog beta-catenin signaling: role in the sebaceous lineage of normal neoplastic mammalian epidermis. Proc. Natl. Acad. Sci. USA 100 Suppl 1:11873-80. 2003-01-01 [PMID: 12917489] (Chemotaxis, WB, Human)

Review for GLI-1 Antibody (NB600-600) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: Verified Customer IHC-P Canine 06/12/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Skin (hair follicle)
• Species: Canine
• Lot: C-2

Comments

• Comments: Antibody dilution = 1:600. 3, 3'-diaminobenzidine (DAB) chromogen and counterstaining with Mayer’s hematoxylin.

Bioinformatics

• Gene Symbol: GLI1
• Entrez:
  • 2735(Human)
  • 14632(Mouse)
• Uniprot:
  • P08151()