GDF-11/BMP-11 Antibody (743833)

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GDF‑11/BMP‑11 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human GDF‑11/BMP‑11 Monoclonal Antibody (Catalog # MAB19581) at 15 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
743833
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

GDF-11/BMP-11 Antibody (743833) Summary

Immunogen
E. coli-derived recombinant human GDF-11/BMP-11
Asn299-Ser407
Accession # O95390
Specificity
Detects human GDF-11/BMP-11 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human BMP-6 or recombinant mouse GDF-8 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GDF11
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB19581 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDF-11/BMP-11 Antibody (743833)

  • BMP-11
  • BMP-11BMP11Bone morphogenetic protein 11
  • GDF11
  • GDF-11
  • growth differentiation factor 11
  • growth/differentiation factor 11

Background

Growth Differentiation Factor 11 (GDF-11), also known as BMP-11, is a member of the TGF-beta superfamily and is highly related to GDF-8. GDF-11 encodes a 407 amino acid (aa) prepropeptide which contains a signal sequence for secretion and an RXXR proteolytic processing site to yield a 109 aa residue carboxy-terminal mature protein (1). Mature GDF-11 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members; however, like the TGF-beta s, Activins and GDF-8, GDF-11 also contains one extra pair of cysteine residues. At the amino acid sequence level, mature human, mouse, rat and chicken GDF-11 are 99‑100% identical. GDF-11 and GDF-8 share 90% amino acid sequence identity within the mature protein. As detected by in situ hybridization, GDF-11 is expressed in diverse regions of the mouse embryo: tailbud, somitic precursors, limbs, mandibular and branchial arches, dorsal neural tube, odontoblasts, nasal epithelium, and particular regions of the brain (1, 2). Likewise, a targeted deletion of GDF-11 in mice results in a spectrum of abnormalities including palatal malformation, vertebral defects, elongated trunks with a reduced or absent tail, missing or malformed kidneys, and an increased number of neurons in the olfactory epithelium (2-5). An intriguing finding in the knockout mice was that the trunk elongation was due to an increase in the number of thoracic vertebrae (4). This implicates GDF-11 as the first secreted factor to influence the specification of segmental identity in vertebrates (3). In fact, GDF-11 does regulate expression of segmental transcription factors, the Hox genes (6). GDF-11 signals through the Activin type II receptors and induces phosphorylation of Smad2 to mediate axial patterning (7). Despite the strong expression in the limb throughout development, no limb abnormalities were found in the knockout mice. However, in vitro micromass studies indicate that GDF-11 inhibits myogenic and chondrogenic cell differentiation and may impact formation and development of the limb skeleton (6).

  1. Gamer, L.W. et al. (1999) Dev. Biol. 208: 222. 
  2. Nakashima, M. et al. (1999) Mech. Dev. 80:185.  
  3. Gad, J.M. and P.P.L. Tam (1999) Curr. Biol. 9:R783.  
  4. McPherron, A.C. et al. (1999) Nat. Genet. 22:260.
  5. Esquela, A.F. and S.J. Lee (2003) Dev. Biol. 257:356.
  6. Gamer, L.W. et al. (2001) Dev. Biol. 229:407.
  7. Oh, S.P. et al. (2002) Genes & Dev. 16:274.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GDF-11/BMP-11 Antibody (MAB19581)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ELISA Development, In Vivo, Western Blot.


Filter By Application
ELISA Development
(1)
In Vivo
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(2)
Mouse
(5)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB19581 Applications Species
Q Jin, C Qiao, J Li, B Xiao, J Li, X Xiao A GDF11/myostatin inhibitor, GDF11 propeptide-Fc, increases skeletal muscle mass and improves muscle strength in dystrophic mdx mice Skelet Muscle, 2019;9(1):16. 2019 [PMID: 31133057] (Western Blot, Human) Western Blot Human
C Ozek, RC Krolewski, SM Buchanan, LL Rubin Growth Differentiation Factor 11 treatment leads to neuronal and vascular improvements in the hippocampus of aged mice Sci Rep, 2018;8(1):17293. 2018 [PMID: 30470794] (Western Blot, Mouse) Western Blot Mouse
Q Jin, C Qiao, J Li, J Li, X Xiao Neonatal Systemic AAV-Mediated Gene Delivery of GDF11 Inhibits Skeletal Muscle Growth Mol. Ther., 2018;26(4):1109-1117. 2018 [PMID: 29503194] (Western Blot, Mouse) Western Blot Mouse
E De Domenic, G D'Arcangel, I Faraoni, M Palmieri, V Tancredi, G Graziani, P Grimaldi, L Tentori Modulation of GDF11 expression and synaptic plasticity by age and training Oncotarget, 2017;8(35):57991-58002. 2017 [PMID: 28938532] (Western Blot, Mouse) Western Blot Mouse
E Latres, J Mastaitis, W Fury, L Miloscio, J Trejos, J Pangilinan, H Okamoto, K Cavino, E Na, A Papatheodo, T Willer, Y Bai, J Hae Kim, A Rafique, S Jaspers, T Stitt, AJ Murphy, GD Yancopoulo, J Gromada Activin A more prominently regulates muscle mass in primates than does GDF8 Nat Commun, 2017;8(0):15153. 2017 [PMID: 28452368] (ELISA Development, Human) ELISA Development Human
H Li, Y Li, L Xiang, J Zhang, B Zhu, L Xiang, J Dong, M Liu, G Xiang GDF11 Attenuates Development of Type 2 Diabetes via Improvement of Islet ? cell Function and Survival Diabetes, 2017;0(0):. 2017 [PMID: 28450417] (In Vivo, Mouse) In Vivo Mouse
DW Hammers, M Merscham-B, JY Hsiao, S Engst, JJ Hartman, HL Sweeney Supraphysiological levels of GDF11 induce striated muscle atrophy EMBO Mol Med, 2017;0(0):. 2017 [PMID: 28270449] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol GDF11
Uniprot