GDF11 Products By Gene Name

1958-GD induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The ED₅₀ for this effect is 0.8‑4.8 ng/mL." class="big_lightbox"> 1958-GD induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The ED₅₀ for this effect is 0.8‑4.8 ng/mL." alt="Recombinant Human/Mouse/Rat GDF‑11 protein 1958-GD induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The ED₅₀ for this effect is 0.8‑4.8 ng/mL." class="big_thumb" />

1958-GD protein was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 13 kDa and 21 kDa, respectively." class="big_lightbox"> 1958-GD protein was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 13 kDa and 21 kDa, respectively." class="big_thumb" />

1958-GD. The peak at 25163 Da corresponds to the calculated molecular mass of the disulfide-linked homodimer." class="big_lightbox"> 1958-GD. The peak at 25163 Da corresponds to the calculated molecular mass of the disulfide-linked homodimer." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to epithelia of the colon. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to epithelia of the colon. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="GDF-11/BMP-11 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human GDF-11/BMP-11 Monoclonal Antibody (Catalog # MAB19581) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to epithelia of the colon. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal root ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal root ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="GDF‑11/BMP‑11 was detected in immersion fixed frozen sections of mouse embryo (11 d.p.c.) using Goat Anti-Human/Mouse GDF‑11/BMP‑11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1958) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal root ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

1958-GD) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the pseudoperoxidase assay. Induction of hemoglobin expression by GDF-11/BMP-11 (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human GDF-11/GDF-8 Monoclonal Antibody (Catalog # MAB19582). The ND₅₀ is typically 0.04-0.2 µg/mL. This antibody also neutralizes GDF-8 in a similar K562 bioassay." class="big_lightbox"> 1958-GD) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the pseudoperoxidase assay. Induction of hemoglobin expression by GDF-11/BMP-11 (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human GDF-11/GDF-8 Monoclonal Antibody (Catalog # MAB19582). The ND₅₀ is typically 0.04-0.2 µg/mL. This antibody also neutralizes GDF-8 in a similar K562 bioassay." alt="Recombinant Human GDF-11/BMP-11 (Catalog # 1958-GD) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the pseudoperoxidase assay. Induction of hemoglobin expression by GDF-11/BMP-11 (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human GDF-11/GDF-8 Monoclonal Antibody (Catalog # MAB19582). The ND₅₀ is typically 0.04-0.2 µg/mL. This antibody also neutralizes GDF-8 in a similar K562 bioassay." class="big_thumb" />

MAB19584) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GDF-11/GDF-8 Monoclonal Antibody (Catalog # MAB19582) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB19584) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GDF-11/GDF-8 Monoclonal Antibody (Catalog # MAB19582) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="GDF-11/BMP-11 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GDF-11 using Mouse Anti-Human GDF-11/BMP-11 Monoclonal Antibody (Catalog # MAB19583) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />