Activin RIIB Antibody

Activin RIIB was detected in immersion fixed paraffin-embedded sections of human placenta (cross-section of chorionic villi) using 5 µg/mL Goat Anti-Human Activin RIIB Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF339) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Myostatin signaling in longissimus dorsi muscle of low-birth-weight (LBWT) and normal-birth-weight (NBWT) neonatal pigs. (A): Representative western blots from four pairs of LBWT and NBWT pigs (N, NBWT; L, LBWT). (B–G): Protein abundance of myostain, ALK5 and ActRIIB, and protein abundance phosphorylation of smad2/3. Abundance was normalized to alpha -tubulin and phosphorylation normalized to the corresponding non-phospho-proteins. Results are means ± SE. n = 12. Values with different letters differ significantly (P ≤ 0.05). Image collected and cropped by CiteAb from the following open publication (//journal.frontiersin.org/article/10.3389/fphys.2017.00482/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Activin RIIB Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Activin RIIB
  Ser19-Thr134
  Accession # CAA54671
• Specificity: Detects human Activin RIIB in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Activin RIIB is observed, and less than 1% cross-reactivity with recombinant human (rh) Activin RIIA, rhActivin RIA, and rhActivin RIB is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACVR2B
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Activin RIIB Antibody

• activin A receptor, type IIB
• Activin receptor type IIB
• activin receptor type-2B
• Activin RIIB
• ActivinRIIB
• ACTRIIB
• ActR-IIB
• ACVR2B
• EC 2.7.11
• EC 2.7.11.30
• MGC116908

Background

Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A) and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists.

1. Attisano, L. et al. (1996) Mol. and Cell Biol. 16:1066.
2. Woodruff, T.K. (1998) Biochem. Pharmacology 55:953.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Activin RIIB Antibody (AF339)(9)

Publications using AF339

• Pramod DHAKAL, Nobuo TSUNODA, Yasuo NAMBO, Hiroyuki TANIYAMA, Kentaro NAGAOKA, Gen WATANABE et al. Circulating activin A during equine gestation and immunolocalization of its receptors system in utero-placental tissues and fetal gonads Journal of Equine Science 2021-06-18 [PMID: 34220270]
• A Dillenburg, G Ireland, RK Holloway, CL Davies, FL Evans, M Swire, ME Bechler, D Soong, TJ Yuen, GH Su, JC Becher, C Smith, A Williams, VE Miron Activin receptors regulate the oligodendrocyte lineage in health and disease Acta Neuropathol., 2018-02-03;0(0):. 2018-02-03 [PMID: 29397421] (Cell Culture, Mouse)
• Y Chen, SR McCauley, SE Johnson, RP Rhoads, SW El-Kadi Downregulated Translation Initiation Signaling Predisposes Low-Birth-Weight Neonatal Pigs to Slower Rates of Muscle Protein Synthesis Front Physiol, 2017-07-11;8(0):482. 7/11/2017 [PMID: 28744224]
• Veronique E. Miron, Amanda Boyd, Jing-Wei Zhao, Tracy J. Yuen, Julia M. Ruckh, Jennifer L. Shadrach et al. M2 microglia and macrophages drive oligodendrocyte differentiation during CNS remyelination Nature Neuroscience 2013-09-01 [PMID: 23872599]
• Bristol-Gould SK, Kreeger PK, Selkirk CG, Kilen SM, Cook RW, Kipp JL, Shea LD, Mayo KE, Woodruff TK Postnatal regulation of germ cells by activin: the establishment of the initial follicle pool. Dev. Biol., 2006-06-17;298(1):132-48. 2006-06-17 [PMID: 16930587] (IHC-P, Mouse)
• Maeshima A, Vaughn DA, Choi Y, Nigam SK Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct. Dev. Biol., 2006-04-27;295(2):473-85. 2006-04-27 [PMID: 16643884] (Western Blot, Rat)
• Fafioffe A, Ethier JF, Fontaine J, JeanPierre E, Taragnat C, Dupont J Activin and inhibin receptor gene expression in the ewe pituitary throughout the oestrous cycle. J. Endocrinol., 2004-07-01;182(1):55-68. 2004-07-01 [PMID: 15225131] (Western Blot, Ovine)
• Bristol SK, Woodruff TK Follicle-restricted compartmentalization of transforming growth factor beta superfamily ligands in the feline ovary. Biol. Reprod., 2003-12-03;70(3):846-59. 2003-12-03 [PMID: 14656728] (IHC-P, Feline)
• Matsuzaki T, Hanai S, Kishi H, Liu Z, Bao Y, Kikuchi A, Tsuchida K, Sugino H Regulation of endocytosis of activin type II receptors by a novel PDZ protein through Ral/Ral-binding protein 1-dependent pathway. J. Biol. Chem., 2002-03-06;277(21):19008-18. 2002-03-06 [PMID: 11882656] (Immunoprecipitation, Mouse)

Review for Activin RIIB Antibody (AF339) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer IHC-P Other 04/02/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: bovine fetal ovary
• Species: Other
• Lot: Bav0413081

Bioinformatics

• Gene Symbol: ACVR2B
• Uniprot:
  • CAA54671()