Western blot shows 25 ng of Recombinant Human BMP‑1/PCP (Catalog # 1927-ZN), Recombinant Human TLL-2, Recombinant Human/Mouse/Rat BMP‑2 (Catalog # 355-BM), Recombinant Human BMP‑3 (Catalog # 113-BP), ...read more
Western blot shows 25 ng of Recombinant Human BMP‑1/PCP (Catalog # 1927-ZN), Recombinant Human TLL-2, Recombinant Human Meprin alpha Subunit/MEP1A (Catalog # 3220-ZN), Recombinant Human Meprin beta ...read more
Mouse myeloma cell line NS0-derived recombinant human BMP‑1/PCP Ala121-Gln730 Accession # NP_001190
Detects human BMP‑1/PCP direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TLL-2 is observed, and less than 1% cross-reactivity with rhBMP-2, -3, -3b, -4, -5, -6, -7, -8, -9, -10, and -15 is observed.
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for BMP-1/PCP Antibody [Unconjugated]
bone morphogenetic protein 1
Mammalian tolloid protein
Bone morphogenetic protein 1 (BMP-1), also known as procollagen C-proteinase (PCP), is a zinc protease of the astacin family (1, 2). BMP-1/PCP plays a key role in formation of extracellular matrix (ECM) by converting precursor proteins into their mature and functional forms. The precursor proteins identified as substrates for BMP-1/PCP include collagens, biglycan, laminin 5, dentin matrix protein-1, and lysyl oxidase (3). There are six alternatively spliced forms known to be derived from the BMP-1 gene, and isoform 1 consisting of residues 1 to 730 was expressed. The secreted and purified protein does not contain the signal peptide (amino acid residues 1‑22) and pro domain (residues 23‑120), but contain protease (residues 121‑321), CUB I (residues 322‑434), CUB II (residues 435‑546), EGF-like (residues 547‑588) and CUB III (residues 591‑703) domains. The pro domain is apparently cleaved by a furin-like proprotein convertase (4). The purified BMP-1/PCP is an active protease and its peptidase activity can be determined as described above. The purified BMP-1/PCP is predicted to possess procollagen C-proteinase activity because it contains the minimal domain structure required (5).
Wozney, J.M. et al. (1988) Science 242:1528.
Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
Steiglitz, B.M. et al. (2004) J. Biol. Chem. 279:980.
Leighton, M. and K.E. Kadler (2003) J. Biol. Chem. 278:18478.
Hartigan, N. et al. (2003) J. Biol. Chem. 278:18045.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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