Activin RIIA Antibody

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Activin RIIA was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human Activin RIIA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF340) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Activin RIIA Antibody Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Activin RIIA
Ser25-Asp82
Accession # P27037
Specificity
Detects human Activin RIIA in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human Activin RIIB is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACVR2A
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
Control
Activin RIIA Knockout HeLa Cell Lysate
Publications
Read Publications using
AF340 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Activin RIIA Antibody

  • activin A receptor, type II
  • activin A receptor, type IIA
  • Activin receptor type IIA
  • activin receptor type-2A
  • Activin RIIA
  • ActivinRIIA
  • ACTRII
  • ACTRIIA
  • ACVR2A
  • ACVR2ACTR-IIA
  • AVR2A
  • EC 2.7.11
  • EC 2.7.11.30

Background

Activin isoforms and other members of the TGF-beta  superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A) and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists.

  1. Attisano, L. et al. (1996) Mol. and Cell. Biol. 16:1066.
  2. Woodruff, T.K. (1998) Biochem. Pharmacology, 55:953.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Activin RIIA Antibody (AF340)(6)

We have publications tested in 4 confirmed species: Mouse, Rat, Feline, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 5 applications: Cell Culture, ELISA Development, IHC-P, Immunoprecipitation, Western Blot.


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Cell Culture
(1)
ELISA Development
(1)
IHC-P
(2)
Immunoprecipitation
(1)
Western Blot
(1)
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Mouse
(3)
Rat
(1)
Feline
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
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Showing Publications 1 - 6 of 6.
Publications using AF340 Applications Species
A Dillenburg, G Ireland, RK Holloway, CL Davies, FL Evans, M Swire, ME Bechler, D Soong, TJ Yuen, GH Su, JC Becher, C Smith, A Williams, VE Miron Activin receptors regulate the oligodendrocyte lineage in health and disease Acta Neuropathol., 2018;0(0):. 2018 [PMID: 29397421] (Cell Culture, Mouse) Cell Culture Mouse
Fajardo RJ, Manoharan RK, Pearsall RS, Davies MV, Marvell T, Monnell TE, Ucran JA, Pearsall AE, Khanzode D, Kumar R, Underwood KW, Roberts B, Seehra J, Bouxsein ML Treatment with a soluble receptor for activin improves bone mass and structure in the axial and appendicular skeleton of female cynomolgus macaques (Macaca fascicularis). Bone, 2010;46(1):64-71. 2010 [PMID: 19781677] (ELISA Development, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)) ELISA Development Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Bristol-Gould SK, Kreeger PK, Selkirk CG, Kilen SM, Cook RW, Kipp JL, Shea LD, Mayo KE, Woodruff TK Postnatal regulation of germ cells by activin: the establishment of the initial follicle pool. Dev. Biol., 2006;298(1):132-48. 2006 [PMID: 16930587] (IHC-P, Mouse) IHC-P Mouse
Maeshima A, Vaughn DA, Choi Y, Nigam SK Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct. Dev. Biol., 2006;295(2):473-85. 2006 [PMID: 16643884] (Western Blot, Rat) Western Blot Rat
Bristol SK, Woodruff TK Follicle-restricted compartmentalization of transforming growth factor beta superfamily ligands in the feline ovary. Biol. Reprod., 2004;70(3):846-59. 2004 [PMID: 14656728] (IHC-P, Feline) IHC-P Feline
Matsuzaki T, Hanai S, Kishi H, Liu Z, Bao Y, Kikuchi A, Tsuchida K, Sugino H Regulation of endocytosis of activin type II receptors by a novel PDZ protein through Ral/Ral-binding protein 1-dependent pathway. J. Biol. Chem., 2002;277(21):19008-18. 2002 [PMID: 11882656] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF340

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Bioinformatics

Gene Symbol ACVR2A
Uniprot