GATA-4 Antibody

Images

 
Western Blot: GATA-4 Antibody [NBP2-16613] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GATA4 antibody diluted at 1:3000.
Immunocytochemistry/ Immunofluorescence: GATA4 Antibody [NBP2-16613] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-16613] - Paraffin-embedded mouse lung. GATA4 antibody diluted at 1:500.
Western Blot: GATA4 Antibody [NBP2-16613] - 50 ug rat heart lysate/extract 10% SDS-PAGE GATA4 antibody dilution: 1:1000.
Western Blot: GATA4 Antibody [NBP2-16613] - Sample (30 ug of whole cell lysate) A: A549 B: HeLa 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: GATA4 Antibody [NBP2-16613] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.73 mg/ml

Order Details

GATA-4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GATA4. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Porcine (93%), Bovine (95%), Canine (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GATA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (80%), Chicken (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.73 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GATA-4 Antibody

  • GATA binding protein 4
  • GATA4
  • GATA-4
  • GATA-binding factor 4
  • GATA-binding protein 4
  • MGC126629
  • transcription factor GATA-4

Background

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GATA4
Entrez
Uniprot