GATA-4 Antibody

Western Blot: GATA-4 Antibody [NBP2-16613] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GATA4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: GATA-4 Antibody [NBP2-16613] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GATA4 stained by GATA4 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.

Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-16613] - Mouse colon. GATA4 stained by GATA4 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/Immunofluorescence: GATA4 Antibody [NBP2-16613] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-16613] - Mouse duodenum. GATA4 stained by GATA4 antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-16613] - Mouse E13.5 embryo.Green: GATA4 stained by GATA4 antibody diluted at 1:250.Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-16613] - Mouse stomach. GATA4 stained by GATA4 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, Po, Bv, Ca
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GATA-4 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human GATA-4. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Porcine (93%), Bovine (95%), Canine (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GATA4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (82%), Chicken (85%), Rat (89%)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GATA-4 Antibody

• GATA binding protein 4
• GATA4
• GATA-4
• GATA-binding factor 4
• GATA-binding protein 4
• MGC126629
• transcription factor GATA-4

Background

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GATA4
• Uniprot:
  • P43694()