Serum Response Factor SRF Antibody (PCRP-SRF-1F7)

Images

 
Immunocytochemistry/ Immunofluorescence: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Serum Response Factor SRF Antibody ...read more
Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - IHC analysis of formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using Serum Response Factor SRF ...read more
Flow Cytometry: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - Flow cytometric analysis of PFA-fixed HeLa cells. Serum Response Factor SRF antibody (PCRP-SRF-1F7) followed by goat anti-mouse IgG-CF488 ...read more
Protein Array: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Serum Response Factor SRF Antibody (PCRP-SRF-1F7).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-P, IP, PA
Clone
PCRP-SRF-1F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Serum Response Factor SRF Antibody (PCRP-SRF-1F7) Summary

Immunogen
Recombinant full-length human Serum Response Factor SRF protein (Uniprot: P11831)
Localization
Nucleus, Nucleoplasm
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SRF
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14188)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Theoretical molecular weight: 40-67kDa

Alternate Names for Serum Response Factor SRF Antibody (PCRP-SRF-1F7)

  • MCM1
  • serum response factor (c-fos serum response element-binding transcriptionfactor)
  • Serum Response Factor SRF
  • serum response factor
  • SRF

Background

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is a cardiac-enriched transcription factor that is required for the appearance of beating sarcomeres in the heart. In addition, SRF may also direct the expression of microRNAs that inhibit the expression of cardiac regulatory factors. SRF binding sites are also constitutive promotor elements in many muscle specific promoters. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk 1 and SRF accessory protein 1 (SAP 1) have DNA binding properties identical to that of p62TCF

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SRF
Uniprot