GATA-6 Antibody [Unconjugated]

Images

 
Western blot shows cytoplasmic and nuclear extracts from PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody ...read more
KATO‑III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA‑6/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human ...read more
GATA‑6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) for 3 hours at room ...read more
GATA‑6 was detected in immersion fixed paraffin-embedded sections of human intestine using Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) at 5 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

GATA-6 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GATA-6
Met1-Thr449
Accession # Q92908
Specificity
Detects human GATA-6 in direct ELISAs and Western blots.  In direct ELISAs, approximately 5% cross-reactivity with recombinant human GATA-5 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GATA6
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 6 Reviews rated 4
using
AF1700 in the following applications:

Publications
Read Publications using
AF1700 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-6 Antibody [Unconjugated]

  • GATA binding protein 6
  • GATA6
  • GATA-6
  • GATA-binding factor 6
  • GATA-binding protein 6
  • transcription factor GATA-6

Background

GATA-6 (also named GATA-GT1) is a zinc finger transcription activating protein that bins to a T/A-G-A-T-A-G/A DNA sequence motif in mesodermally and endodermally-derived tissue. There are two GATA-6 isoforms that arise from alternate start sites: a short form of 449 amino acids (aa), and a long form of 595 aa. The short form of human GATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat and mouse, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-24585
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF1700
Species: Hu
Applications: WB, IHC, ChIP, ICC
NBP1-87992
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-24585
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF2170
Species: Hu
Applications: WB, ChIP, ICC
NB100-2379
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, ICC/IF, IHC
AF1997
Species: Hu
Applications: WB, ChIP, ICC
H00388112-B01P
Species: Hu
Applications: WB, ELISA
NB100-2136
Species: Hu
Applications: WB (-), IHC, IHC-P, IP
NBP1-52823
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-44501
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, IF
NBP1-31558
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
MAB1368
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICC, ICFlow, KO
MAB2018
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-reported, ICC, ICFlow
AF2400
Species: Hu
Applications: WB, IHC, ChIP, ICC
NBP2-27406
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF2605
Species: Hu
Applications: WB, Simple Western, IHC, ICC
AF2170
Species: Hu
Applications: WB, ChIP, ICC
AF1700
Species: Hu
Applications: WB, IHC, ChIP, ICC/IF

Publications for GATA-6 Antibody (AF1700)(31)

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 7 applications: ChIP, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(2)
ICC
(14)
IHC
(4)
IHC-Fr
(3)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(3)
Mouse
(25)
Bovine
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using AF1700 Applications Species
S Futaki, I Nakano, M Kawasaki, N Sanzen, K Sekiguchi Molecular profiling of the basement membrane of pluripotent epiblast cells in post-implantation stage mouse embryos Regen Ther, 2019;12(0):55-65. 2019 [PMID: 31890767] (IHC-Fr, Mouse) IHC-Fr Mouse
N Gago-Lopez, LF Mellor, D Megías, G Martín-Ser, A Izeta, F Jimenez, EF Wagner Role of bulge epidermal stem cells and TSLP signaling in psoriasis EMBO Mol Med, 2019;0(0):e10697. 2019 [PMID: 31556482] (IHC-P, Mouse) IHC-P Mouse
W Zhang, C Chronis, X Chen, H Zhang, R Spalinskas, M Pardo, L Chen, G Wu, Z Zhu, Y Yu, L Yu, J Choudhary, J Nichols, MM Parast, B Greber, P Sahlén, K Plath The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation Cell Stem Cell, 2019;24(1):138-152.e8. 2019 [PMID: 30609396] (Western Blot, Mouse) Western Blot Mouse
S Frank, G Ahuja, D Bartsch, N Russ, W Yao, JC Kuo, JP Derks, VS Akhade, Y Kargapolov, T Georgomano, JE Messling, M Gramm, L Brant, R Rehimi, NE Vargas, A Kuroczik, TP Yang, RGA Sahito, J Franzen, J Hescheler, A Sachinidis, M Peifer, A Rada-Igles, M Kanduri, IG Costa, C Kanduri, A Papantonis, L Kurian yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30554961] (ICC, Mouse) ICC Mouse
Y Kang, Z Ai, K Duan, C Si, Y Wang, Y Zheng, J He, Y Yin, S Zhao, B Niu, X Zhu, L Liu, L Xiang, L Zhang, Y Niu, W Ji, T Li Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys Cell Rep, 2018;25(9):2563-2576.e9. 2018 [PMID: 30485820] (ICC, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)) ICC Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
AD Senft, I Costello, HW King, AW Mould, EK Bikoff, EJ Robertson Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming Cell Rep, 2018;24(8):1977-1985.e7. 2018 [PMID: 30134160]
Q Liu, G Wang, Y Lyu, M Bai, Z Jiapaer, W Jia, T Han, R Weng, Y Yang, Y Yu, J Kang The miR-590/Acvr2a/Terf1 Axis Regulates Telomere Elongation and Pluripotency of Mouse iPSCs Stem Cell Reports, 2018;0(0):. 2018 [PMID: 29910124] (ICC, Mouse) ICC Mouse
K Simmet, V Zakhartche, J Philippou-, H Blum, N Klymiuk, E Wolf OCT4/POU5F1 is required for NANOG expression in bovine blastocysts Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 29483258] (IHC, Bovine) IHC Bovine
Y Meng, R Moore, W Tao, ER Smith, JD Tse, C Caslini, XX Xu GATA6 phosphorylation by Erk1/2 propels exit from pluripotency and commitment to primitive endoderm Dev. Biol., 2018;0(0):. 2018 [PMID: 29454706] (ChIP, Mouse) ChIP Mouse
S Bessonnard, S Coqueran, S Vandormael, A Dufour, J Artus, M Cohen-Tann ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation Sci Rep, 2017;7(1):12285. 2017 [PMID: 28947813] (IHC, Mouse) IHC Mouse
Show All 31 Publications.

Reviews for GATA-6 Antibody (AF1700) (6) 46

Average Rating: 4
(Based on 6 reviews)
We have 6 reviews tested in 1 species: Mouse.

Reviews using AF1700:
Filter by Applications
ICC
(1)
IHC-P
(2)
IF
(2)
ChIP
(1)
All Applications
Filter by Species
Mouse
(6)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry GATA-6 AF1700
Enlarge
reviewed by:
Anonymous
ICC Mouse 08/04/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedR1 wild type Mouse embryonic Stem Cells
SpeciesMouse
LotKWT0214121

Blocking

Blocking DetailsBlocking: 0.1% Triton X in PBS with 5% donkey serum, 1 hr at RT

Primary Anitbody

Dilution Ratio1:200, 14 hrs, 4 C, Buffer: 0.1% Triton X + 5% donkey serum in PBS

Secondary Antibody

Secondary DescriptionDonkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 647 conjugate
Secondary Manufacturer Cat#A-21447
Secondary Concentration1:1000

Details

Detection NotesFluorescence Imaging, AF647 with cy5 filter cube, Nikon Eclipse Ti microscope, Exposure time: 1 sec
Fixation DetailsFixed for 10 mins with 4% formaldehyde at room temperature. Permeablization with 0.2% TritonX in PBS for 15 mins at RT
Wash DescriptionPBS,~1 min, 3 times

Comments

CommentsCells were cultured for a day in SerumLif before switching to endoderm differentiation conditions where Gata6 is expressed. Fixation after 3 days in differentiation conditions.
 
reviewed by:
Anonymous
IHC-P Mouse 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23002247
SpeciesMouse
 
reviewed by:
Anonymous
IHC-P Mouse 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22733991
SpeciesMouse
 
reviewed by:
Anonymous
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22733991
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22733991

Details

Detection NotesSee PMID 22733991

Comments

CommentsPublished: See PMID 22733991
 
reviewed by:
Anonymous
ChIP Mouse 01/05/2015
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedSee PMID 23002247
SpeciesMouse

Details

Detection NotesSee PMID 23002247

Comments

Comments"1ug of antibody per 500ug-1mg of chromatingood enrichment at target sites"See PMID 23002247
 
reviewed by:
Anonymous
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 20826529
SpeciesMouse

Blocking

Blocking DetailsSee PMID 20826529

Details

Detection NotesSee PMID 20826529

Comments

CommentsSamples were fixed in 4% formaldehyde and antibody was used at 1/200 dilution.Published: See PMID 20826529

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GATA-6 Antibody (AF1700) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1700

Additional GATA-6 Products

Bioinformatics Tool for GATA-6 Antibody (AF1700)

Discover related pathways, diseases and genes to GATA-6 Antibody (AF1700). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GATA-6 Antibody (AF1700)

Discover more about diseases related to GATA-6 Antibody (AF1700).
 

Pathways for GATA-6 Antibody (AF1700)

View related products by pathway.

PTMs for GATA-6 Antibody (AF1700)

Learn more about PTMs related to GATA-6 Antibody (AF1700).

Blogs on GATA-6

There are no specific blogs for GATA-6, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
1
4
4
3
1
2
0
1
0

Anonymous
08/04/2016
Application: ICC
Species: Mouse

 
Anonymous
01/05/2015
Application: IHC-P
Species: Mouse

 
Anonymous
01/05/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol GATA6
Uniprot
COVID-19 update