GATA-6 Antibody [Unconjugated]

Western blot shows cytoplasmic and nuclear extracts from PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA‑6 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GATA‑6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

KATO‑III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA‑6/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The mucin4 promoter was detected by standard PCR.

GATA‑6 was detected in immersion fixed paraffin-embedded sections of human intestine using Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, ChIP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GATA-6 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human GATA-6
  Met1-Thr449
  Accession # Q92908
• Specificity: Detects human GATA-6 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GATA6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-6 Antibody [Unconjugated]

• GATA binding protein 6
• GATA6
• GATA-6
• GATA-binding factor 6
• GATA-binding protein 6
• transcription factor GATA-6

Background

GATA-6 (also named GATA-GT1) is a zinc finger transcription activating protein that bins to a T/A-G-A-T-A-G/A DNA sequence motif in mesodermally and endodermally-derived tissue. There are two GATA-6 isoforms that arise from alternate start sites: a short form of 449 amino acids (aa), and a long form of 595 aa. The short form of human GATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat and mouse, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GATA-6 Antibody (AF1700)(61)

Publications using AF1700

• Toyooka, Y;Aoki, K;Usami, FM;Oka, S;Kato, A;Fujimori, T; Generation of pulsatile ERK activity in mouse embryonic stem cells is regulated by Raf activity Scientific reports 2023-06-10 [PMID: 37301878] (ICC, Transgenic Mouse)
• V Garg, Y Yang, S Nowotschin, M Setty, YY Kuo, R Sharma, A Polyzos, E Salataj, D Murphy, A Jang, D Pe'er, E Apostolou, AK Hadjantona Single-cell analysis of bidirectional reprogramming between early embryonic states reveals mechanisms of differential lineage plasticities bioRxiv : the preprint server for biology, 2023-03-29;0(0):. 2023-03-29 [PMID: 37034770] (ChIP, Flow Cytometry, ICC, Mouse)
• P Athanasoul, M Balli, A De Jaime-S, A Boel, S Papanikola, BK van der Ve, A Janiszewsk, T Vanhessche, A Francis, Y El Laithy, AL Nigro, F Aulicino, KP Koh, V Pasque, MP Cosma, C Verfaillie, A Zwijsen, B Heindryckx, C Nikolaou, F Lluis The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Nature Communications, 2023-03-03;14(1):1210. 2023-03-03 [PMID: 36869101] (ICC, Mouse)
• C Dupont, OJM Schäffers, BF Tan, S Merzouk, EM Bindels, A Zwijsen, D Huylebroec, J Gribnau Efficient generation of ETX embryoids that recapitulate the entire window of murine egg cylinder development Science Advances, 2023-01-18;9(3):eadd2913. 2023-01-18 [PMID: 36652512] (IHC, Mouse)
• D Li, J Yang, V Malik, Y Huang, X Huang, H Zhou, J Wang An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity Nucleic Acids Research, 2022-11-28;0(0):. 2022-11-28 [PMID: 36416264] (ICC, Mouse)
• JJ Thompson, DJ Lee, A Mitra, S Frail, RK Dale, PP Rocha Extensive co-binding and rapid redistribution of NANOG and GATA6 during emergence of divergent lineages Nature Communications, 2022-07-23;13(1):4257. 2022-07-23 [PMID: 35871075] (IHC, Mouse)
• NM Bérenger-C, M Mircea, E Adegeest, PR van den Be, M Feliksik, M Hochane, T Idema, SJ Tans, S Semrau A gastruloid model of the interaction between embryonic and extra-embryonic cell types Journal of tissue engineering, 2022-06-11;13(0):2041731422110. 2022-06-11 [PMID: 35707767] (IHC, Mouse)
• Y Bi, Z Tu, J Zhou, X Zhu, H Wang, S Gao, Y Wang Cell fate roadmap of human primed-to-naive transition reveals preimplantation cell lineage signatures Nature Communications, 2022-06-07;13(1):3147. 2022-06-07 [PMID: 35672317] (ICC, Human)
• L Trilla-Fue, A Gámez-Pozo, MI Lumbreras-, R López-Vaca, V Heredia-So, I Ghanem, E López-Cama, A Zapater-Mo, M Miguel, EM Peña-Burgo, E Palacios, M De Uribe, L Guerra, A Dittmann, M Mendiola, JÁ Fresno Var, J Feliu Identification of Carcinogenesis and Tumor Progression Processes in Pancreatic Ductal Adenocarcinoma Using High-Throughput Proteomics Cancers, 2022-05-13;14(10):. 2022-05-13 [PMID: 35626021] (IHC, Human)
• V Trappetti, J Fazzari, C Fernandez-, L Smyth, M Potez, N Shintani, B de Breuyn, OA Martin, V Djonov Targeted Accumulation of Macrophages Induced by Microbeam Irradiation in a Tissue-Dependent Manner Biomedicines, 2022-03-22;10(4):. 2022-03-22 [PMID: 35453485] (IHC, Mouse)
• C Royer, E Sandham, E Slee, F Schneider, CB Lagerholm, J Godwin, N Veits, H Hathrell, F Zhou, K Leonaviciu, J Garratt, T Narendra, A Vincent, C Jones, T Child, K Coward, C Graham, M Fritzsche, X Lu, S Srinivas ASPP2 maintains the integrity of mechanically stressed pseudostratified epithelia during morphogenesis Nature Communications, 2022-02-17;13(1):941. 2022-02-17 [PMID: 35177595] (IHC, Mouse)
• D Han, G Wu, R Chen, HCA Drexler, CM MacCarthy, KP Kim, K Adachi, D Gerovska, L Mavrommati, I Bedzhov, MJ Araúzo-Bra, HR Schöler A balanced Oct4 interactome is crucial for maintaining pluripotency Science Advances, 2022-02-16;8(7):eabe4375. 2022-02-16 [PMID: 35171666] (IHC, Mouse)
• ZY Zuo, GH Yang, HY Wang, SY Liu, YJ Zhang, Y Cai, F Chen, H Dai, Y Xiao, MB Cheng, Y Huang, Y Zhang Klf4 methylated by Prmt1 restrains the commitment of primitive endoderm Nucleic Acids Research, 2022-02-28;0(0):. 2022-02-28 [PMID: 35137179] (ICC, Mouse)
• N Rodina, A Khudiakov, K Perepelina, A Muravyev, A Boytsov, A Zlotina, P Sokolnikov, A Kostareva Generation of iPSC line (FAMRCi009-A) from patient with familial progressive cardiac conduction disorder carrying genetic variant FLNC p.Val2264Met Stem Cell Research, 2021-12-27;59(0):102640. 2021-12-27 [PMID: 34971933] (Flow Cytometry, ICC, Human)
• K Perepelina, A Khudiakov, N Rodina, A Boytsov, T Vavilova, A Zlotina, P Sokolnikov, A Kostareva Generation of iPSC line FAMRCi010-A from patient with restrictive cardiomyopathy carrying genetic variant FLNC p.Gly2011Arg Stem Cell Research, 2021-12-21;59(0):102639. 2021-12-21 [PMID: 34971931] (Flow Cytometry, ICC, Human)
• K Duan, CY Si, SM Zhao, ZY Ai, BH Niu, Y Yin, LF Xiang, H Ding, Y Zheng The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey Cells, 2021-10-09;10(10):. 2021-10-09 [PMID: 34685690] (ICC/IF, Cynomolgus Monkey)
• T Pachano, V Sánchez-Ga, T Ealo, M Mariner-Fa, T Bleckwehl, HG Asenjo, P Respuela, S Cruz-Molin, M Muñoz-San, E Haro, WFJ van IJcken, D Landeira, A Rada-Igles Orphan CpG islands amplify poised enhancer regulatory activity and determine target gene responsiveness Nature Genetics, 2021-06-28;53(7):1036-1049. 2021-06-28 [PMID: 34183853] (IHC, Mouse)
• A Zhao, H Qin, M Sun, M Tang, J Mei, K Ma, X Fu Chemical conversion of human epidermal stem cells into intestinal goblet cells for modeling mucus-microbe interaction and therapy Science Advances, 2021-04-14;7(16):. 2021-04-14 [PMID: 33853767] (ICC, Human)
• HS Lee, E Kim, J Lee, SJ Park, HK Hwang, CH Park, SY Jo, CM Kang, SM Hong, H Kang, JH Jo, IR Cho, MJ Chung, JY Park, SW Park, SY Song, JM Han, S Kim, S Bang Profiling of conditionally reprogrammed cell lines for in vitro chemotherapy response prediction of pancreatic cancer EBioMedicine, 2021-02-25;65(0):103218. 2021-02-25 [PMID: 33639403] (IHC, Human)
• X Wang, Y Xiang, Y Yu, R Wang, Y Zhang, Q Xu, H Sun, ZA Zhao, X Jiang, X Wang, X Lu, D Qin, Y Quan, J Zhang, N Shyh-Chang, H Wang, N Jing, W Xie, L Li Formative pluripotent stem cells show features of epiblast cells poised for gastrulation Cell Research, 2021-02-19;0(0):. 2021-02-19 [PMID: 33608671] (ICC, IHC, Mouse)
• A Weberling, M Zernicka-G Trophectoderm mechanics direct epiblast shape upon embryo implantation Cell Reports, 2021-01-19;34(3):108655. 2021-01-19 [PMID: 33472064] (IHC, Mouse)
• Y Li, H Li, X Wei Long noncoding RNA LINC00261 suppresses prostate cancer tumorigenesis through upregulation of GATA6-mediated DKK3 Cancer Cell Int, 2020-09-30;20(0):474. 2020-09-30 [PMID: 33013201] (ChIP, Immunoprecipitation, Human)
• KP Kim, Y Wu, J Yoon, K Adachi, G Wu, S Velychko, CM MacCarthy, B Shin, A Röpke, MJ Arauzo-Bra, M Stehling, DW Han, Y Gao, J Kim, S Gao, HR Schöler Reprogramming competence of OCT factors is determined by transactivation domains Science Advances, 2020-09-02;6(36):. 2020-09-02 [PMID: 32917606] (ICC, Mouse)
• MN Shahbazi, T Wang, X Tao, BAT Weatherbee, L Sun, Y Zhan, L Keller, GD Smith, A Pellicer, RT Scott, E Seli, M Zernicka-G Developmental potential of aneuploid human embryos cultured beyond implantation Nat Commun, 2020-08-10;11(1):3987. 2020-08-10 [PMID: 32778678] (ICC, IHC, Human)
• K Perepelina, A Kostina, P Klauzen, A Khudiakov, M Rabino, S Crasto, A Zlotina, Y Fomicheva, A Sergushich, M Oganesian, A Dmitriev, A Kostareva, E Di Pasqual, A Malashiche Generation of two iPSC lines (FAMRCi007-A and FAMRCi007-B) from patient with Emery-Dreifuss muscular dystrophy and heart rhythm abnormalities carrying genetic variant LMNA p.Arg249Gln Stem Cell Res, 2020-06-29;47(0):101895. 2020-06-29 [PMID: 32659731] (Flow Cytometry, ICC, Human)
• Y Ueda, C Kimura-Yos, K Mochida, M Tsume, Y Kameo, T Adachi, O Lefebvre, R Hiramatsu, I Matsuo Intrauterine Pressures Adjusted by Reichert's Membrane Are Crucial for Early Mouse Morphogenesis Cell Rep, 2020-05-19;31(7):107637. 2020-05-19 [PMID: 32433954] (IHC, Mouse)
• P Klauzen, K Perepelina, A Khudiakov, A Zlotina, Y Fomicheva, T Pervunina, T Vershinina, A Kostareva, A Malashiche Generation of two induced pluripotent stem cell lines (FAMRCi005-A and FAMRCi005-B) from patient carrying genetic variant LMNA p.Asp357Val Stem Cell Res, 2020-02-05;43(0):101719. 2020-02-05 [PMID: 32062135] (ICC, Human)
• A Khudiakov, K Perepelina, P Klauzen, A Zlotina, K Gusev, E Kaznacheye, A Malashiche, A Kostareva Generation of two iPSC lines (FAMRCi004-A and FAMRCi004-B) from patient with familial progressive cardiac conduction disorder carrying genetic variant DSP p.His1684Arg Stem Cell Res, 2020-02-04;43(0):101720. 2020-02-04 [PMID: 32062131] (Flow Cytometry, IF/ICC, Human)
• K Perepelina, P Klauzen, A Khudiakov, A Zlotina, Y Fomicheva, D Rudenko, M Gordeev, A Sergushich, A Malashiche, A Kostareva Generation of two iPSC lines (FAMRCi006-A and FAMRCi006-B) from patient with dilated cardiomyopathy and Emery-Dreifuss muscular dystrophy associated with genetic variant LMNAp.Arg527Pro Stem Cell Res, 2020-01-31;43(0):101714. 2020-01-31 [PMID: 32059175] (ICC, Human)
• S Futaki, I Nakano, M Kawasaki, N Sanzen, K Sekiguchi Molecular profiling of the basement membrane of pluripotent epiblast cells in post-implantation stage mouse embryos Regen Ther, 2019-05-10;12(0):55-65. 2019-05-10 [PMID: 31890767] (IHC-Fr, Mouse)
• N Gago-Lopez, LF Mellor, D Megías, G Martín-Ser, A Izeta, F Jimenez, EF Wagner Role of bulge epidermal stem cells and TSLP signaling in psoriasis EMBO Mol Med, 2019-09-26;0(0):e10697. 2019-09-26 [PMID: 31556482] (IHC-P, Mouse)
• W Zhang, C Chronis, X Chen, H Zhang, R Spalinskas, M Pardo, L Chen, G Wu, Z Zhu, Y Yu, L Yu, J Choudhary, J Nichols, MM Parast, B Greber, P Sahlén, K Plath The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation Cell Stem Cell, 2019-01-03;24(1):138-152.e8. 2019-01-03 [PMID: 30609396] (Western Blot, Mouse)
• S Frank, G Ahuja, D Bartsch, N Russ, W Yao, JC Kuo, JP Derks, VS Akhade, Y Kargapolov, T Georgomano, JE Messling, M Gramm, L Brant, R Rehimi, NE Vargas, A Kuroczik, TP Yang, RGA Sahito, J Franzen, J Hescheler, A Sachinidis, M Peifer, A Rada-Igles, M Kanduri, IG Costa, C Kanduri, A Papantonis, L Kurian yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs Cell Stem Cell, 2018-12-13;0(0):. 2018-12-13 [PMID: 30554961] (ICC, Mouse)
• Y Kang, Z Ai, K Duan, C Si, Y Wang, Y Zheng, J He, Y Yin, S Zhao, B Niu, X Zhu, L Liu, L Xiang, L Zhang, Y Niu, W Ji, T Li Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys Cell Rep, 2018-11-27;25(9):2563-2576.e9. 2018-11-27 [PMID: 30485820] (ICC, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• AD Senft, I Costello, HW King, AW Mould, EK Bikoff, EJ Robertson Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming Cell Rep, 2018-08-21;24(8):1977-1985.e7. 2018-08-21 [PMID: 30134160]
• Q Liu, G Wang, Y Lyu, M Bai, Z Jiapaer, W Jia, T Han, R Weng, Y Yang, Y Yu, J Kang The miR-590/Acvr2a/Terf1 Axis Regulates Telomere Elongation and Pluripotency of Mouse iPSCs Stem Cell Reports, 2018-06-14;0(0):. 2018-06-14 [PMID: 29910124] (ICC, Mouse)
• K Simmet, V Zakhartche, J Philippou-, H Blum, N Klymiuk, E Wolf OCT4/POU5F1 is required for NANOG expression in bovine blastocysts Proc. Natl. Acad. Sci. U.S.A., 2018-02-26;0(0):. 2018-02-26 [PMID: 29483258] (IHC, Bovine)
• Y Meng, R Moore, W Tao, ER Smith, JD Tse, C Caslini, XX Xu GATA6 phosphorylation by Erk1/2 propels exit from pluripotency and commitment to primitive endoderm Dev. Biol., 2018-02-15;0(0):. 2018-02-15 [PMID: 29454706] (ChIP, IHC, Mouse)
• S Bessonnard, S Coqueran, S Vandormael, A Dufour, J Artus, M Cohen-Tann ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation Sci Rep, 2017-09-25;7(1):12285. 2017-09-25 [PMID: 28947813] (IHC, Mouse)
• X Li, D Liu, Y Ma, X Du, J Jing, L Wang, B Xie, D Sun, S Sun, X Jin, X Zhang, T Zhao, J Guan, Z Yi, W Lai, P Zheng, Z Huang, Y Chang, Z Chai, J Xu, H Deng Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State Cell Stem Cell, 2017-06-22;0(0):. 2017-06-22 [PMID: 28648365] (ICC, Mouse)
• M Kang, V Garg, AK Hadjantona Lineage Establishment and Progression within the Inner Cell Mass of the Mouse Blastocyst Requires FGFR1 and FGFR2 Dev. Cell, 2017-05-25;0(0):. 2017-05-25 [PMID: 28552559] (ICC, Mouse)
• A Molotkov, P Mazot, JR Brewer, RM Cinalli, P Soriano Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency Dev. Cell, 2017-05-25;0(0):. 2017-05-25 [PMID: 28552557] (ICC, Mouse)
• M Goolam, M Zernicka-G The chromatin modifier Satb1 regulates cell fate through Fgf signalling in the early mouse embryo Development, 2017-03-13;0(0):. 2017-03-13 [PMID: 28289135] (ICC, Mouse)
• Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos Sci Rep, 2016-12-19;6(0):39457. 2016-12-19 [PMID: 27991575] (ICC, Mouse)
• Abrogation Of Gap Junctional Communication In Es Cells Results In A Disruption Of Primitive Endoderm Formation In Embryoid Bodies Stem Cells, 2016-12-20;0(0):. 2016-12-20 [PMID: 27870307] (IHC-Fr, Mouse)
• Asynchronous fate decisions by single cells collectively ensure consistent lineage composition in the mouse blastocyst Nat Commun, 2016-11-18;7(0):13463. 2016-11-18 [PMID: 27857135] (IHC-Fr, Mouse)
• p38 (Mapk14/11) occupies a regulatory node governing entry into primitive endoderm differentiation during preimplantation mouse embryo development Open Biol, 2016-09-01;6(9):. 2016-09-01 [PMID: 27605380] (IHC, Mouse)
• L Song, J Chen, G Peng, K Tang, N Jing Dynamic Heterogeneity of Brachyury in Mouse Epiblast Stem Cells Mediates Distinct Response to Extrinsic Bone Morphogenetic Protein (BMP) Signaling J. Biol. Chem., 2016-05-16;291(29):15212-25. 2016-05-16 [PMID: 27226536] (ICC, Mouse)
• Sun , Congshan, Denisenko , Oleg, Sheth , Bhavwant, Cox , Andy, Lucas , Emma S, Smyth , Neil R, Fleming , Tom P Epigenetic regulation of histone modifications and Gata6 gene expression induced by maternal diet in mouse embryoid bodies in a model of developmental programming. BMC Dev Biol, 2015-01-21;15(0):3. 2015-01-21 [PMID: 25609498] (Western Blot, Mouse)
• Shu , Jian, Zhang , Ke, Zhang , Minjie, Yao , Anzhi, Shao , Sida, Du , Fengxia, Yang , Caiyun, Chen , Wenhan, Wu , Chen, Yang , Weifeng, Sun , Yingli, Deng , Hongkui GATA family members as inducers for cellular reprogramming to pluripotency. Cell Res, 2015-01-16;25(2):169-80. 2015-01-16 [PMID: 25591928] (Immunoprecipitation, Mouse)
• Krawchuk D, Anani S, Honma-Yamanaka N, Polito S, Shafik M, Yamanaka Y Loss of LKB1 leads to impaired epithelial integrity and cell extrusion in the early mouse embryo. J Cell Sci, 2015-01-14;128(5):1011-22. 2015-01-14 [PMID: 25588837] (IHC, Mouse)
• Tan F, Qian C, Tang K, Abd-Allah S, Jing N Inhibition of transforming growth factor beta (TGF-beta) signaling can substitute for Oct4 protein in reprogramming and maintain pluripotency. J Biol Chem, 2014-12-29;290(7):4500-11. 2014-12-29 [PMID: 25548277] (ICC, Human)
• Graham , Sarah J, Wicher , Krzyszto, Jedrusik , Agnieszk, Guo , Guoji, Herath , Wishva, Robson , Paul, Zernicka-Goetz , Magdalen BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo. Nat Commun, 2014-12-16;5(0):5667. 2014-12-16 [PMID: 25514175] (ICC, Mouse)
• Schrode N, Saiz N, Di Talia S, Hadjantonakis A GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst. Dev Cell, 2014-05-15;29(4):454-67. 2014-05-15 [PMID: 24835466] (IHC, Mouse)
• Doughton , Gail, Wei , Jun, Tapon , Nicolas, Welham , Melanie, Chalmers , Andrew D Formation of a polarised primitive endoderm layer in embryoid bodies requires fgfr/erk signalling. PLoS ONE, 2014-04-21;9(4):e95434. 2014-04-21 [PMID: 24752320] (ICC, Western Blot, Mouse)
• Sun , Congshan, Velazquez , Miguel A, Marfy-Smith , Stephani, Sheth , Bhavwant, Cox , Andy, Johnston , David A, Smyth , Neil, Fleming , Tom P Mouse early extra-embryonic lineages activate compensatory endocytosis in response to poor maternal nutrition. Development, 2014-02-06;141(5):1140-50. 2014-02-06 [PMID: 24504338] (ICC, Western Blot, Mouse)
• Froese N, Kattih B, Breitbart A, Grund A, Geffers R, Molkentin JD, Kispert A, Wollert KC, Drexler H, Heineke J GATA6 Promotes Angiogenic Function and Survival in Endothelial Cells by Suppression of Autocrine Transforming Growth Factor beta/Activin Receptor-like Kinase 5 Signaling. J. Biol. Chem., 2010-12-02;286(7):5680-90. 2010-12-02 [PMID: 21127043] (ICC, Western Blot, Human)
• Artus J, Panthier JJ, Hadjantonakis AK A role for PDGF signaling in expansion of the extra-embryonic endoderm lineage of the mouse blastocyst. Development, 2010-09-08;137(20):3361-72. 2010-09-08 [PMID: 20826533] (ICC, Mouse)
• Zhang Y, Goss AM, Cohen ED, Kadzik R, Lepore JJ, Muthukumaraswamy K, Yang J, DeMayo FJ, Whitsett JA, Parmacek MS, Morrisey EE A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration. Nat. Genet., 2008-06-08;40(7):862-70. 2008-06-08 [PMID: 18536717] (ICC, Mouse)
• Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD New cell lines from mouse epiblast share defining features with human embryonic stem cells. Nature, 2007-06-27;448(7150):196-9. 2007-06-27 [PMID: 17597760] (ICC, Mouse)
• Jonckheere N, Vincent A, Perrais M, Ducourouble MP, Male AK, Aubert JP, Pigny P, Carraway KL, Freund JN, Renes IB, Van Seuningen I The human mucin MUC4 is transcriptionally regulated by caudal-related homeobox, hepatocyte nuclear factors, forkhead box A, and GATA endodermal transcription factors in epithelial cancer cells. J. Biol. Chem., 2007-06-06;282(31):22638-50. 2007-06-06 [PMID: 17553805] (ChIP, Human)

Reviews for GATA-6 Antibody (AF1700) (6)

Average Rating: 4

(Based on 6 reviews)

We have 6 reviews tested in 1 species: Mouse.

reviewed by: Simon Hastreiter ICC Mouse 08/04/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: R1 wild type Mouse embryonic Stem Cells
• Species: Mouse
• Lot: KWT0214121

reviewed by: Anonymous - IHC-P Mouse 01/05/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 23002247
• Species: Mouse

reviewed by: Anonymous - IHC-P Mouse 01/05/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 22733991
• Species: Mouse

reviewed by: Anonymous - IF Mouse 01/05/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22733991
• Species: Mouse

reviewed by: Anonymous - ChIP Mouse 01/05/2015

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: See PMID 23002247
• Species: Mouse

reviewed by: Anonymous - IF Mouse 01/05/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 20826529
• Species: Mouse

Bioinformatics

• Gene Symbol: GATA6
• Uniprot:
  • Q92908()