GATA-6 Antibody [Unconjugated]

Images

 
GATA‑6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) for 3 hours at room ...read more
KATO‑III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA‑6/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human ...read more
Western blot shows cytoplasmic and nuclear extracts from PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody ...read more
GATA‑6 was detected in immersion fixed paraffin-embedded sections of human intestine using Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) at 5 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

GATA-6 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GATA-6
Met1-Thr449
Accession # Q92908
Specificity
Detects human GATA-6 in direct ELISAs and Western blots.  In direct ELISAs, approximately 5% cross-reactivity with recombinant human GATA-5 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GATA6
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 6 Reviews rated 4
using
AF1700 in the following applications:

Publications
Read Publications using
AF1700 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-6 Antibody [Unconjugated]

  • GATA binding protein 6
  • GATA6
  • GATA-6
  • GATA-binding factor 6
  • GATA-binding protein 6
  • transcription factor GATA-6

Background

GATA-6 (also named GATA-GT1) is a zinc finger transcription activating protein that bins to a T/A-G-A-T-A-G/A DNA sequence motif in mesodermally and endodermally-derived tissue. There are two GATA-6 isoforms that arise from alternate start sites: a short form of 449 amino acids (aa), and a long form of 595 aa. The short form of human GATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat and mouse, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GATA-6 Antibody (AF1700)(24)

We have publications tested in 3 confirmed species: Human, Mouse, Bovine.

We have publications tested in 7 applications: ChIP, ICC, IHC, IHC Fresh, IHC-Fluorescent, IP, WB.


Filter By Application
ChIP
(1)
ICC
(12)
IHC
(3)
IHC Fresh
(2)
IHC-Fluorescent
(1)
IP
(1)
WB
(4)
All Applications
Filter By Species
Human
(3)
Mouse
(20)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using AF1700 Applications Species
Q Liu, G Wang, Y Lyu, M Bai, Z Jiapaer, W Jia, T Han, R Weng, Y Yang, Y Yu, J Kang The miR-590/Acvr2a/Terf1 Axis Regulates Telomere Elongation and Pluripotency of Mouse iPSCs Stem Cell Reports, 2018;0(0):. 2018 [PMID: 29910124] (ICC,, Mouse) ICC Mouse
K Simmet, V Zakhartche, J Philippou-, H Blum, N Klymiuk, E Wolf OCT4/POU5F1 is required for NANOG expression in bovine blastocysts Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 29483258] (IHC,, Bovine) IHC Bovine
S Bessonnard, S Coqueran, S Vandormael, A Dufour, J Artus, M Cohen-Tann ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation Sci Rep, 2017;7(1):12285. 2017 [PMID: 28947813] (IHC,, Mouse) IHC Mouse
X Li, D Liu, Y Ma, X Du, J Jing, L Wang, B Xie, D Sun, S Sun, X Jin, X Zhang, T Zhao, J Guan, Z Yi, W Lai, P Zheng, Z Huang, Y Chang, Z Chai, J Xu, H Deng Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28648365] (ICC,, Mouse) ICC Mouse
M Kang, V Garg, AK Hadjantona Lineage Establishment and Progression within the Inner Cell Mass of the Mouse Blastocyst Requires FGFR1 and FGFR2 Dev. Cell, 2017;0(0):. 2017 [PMID: 28552559] (ICC,, Mouse) ICC Mouse
A Molotkov, P Mazot, JR Brewer, RM Cinalli, P Soriano Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency Dev. Cell, 2017;0(0):. 2017 [PMID: 28552557] (ICC,, Mouse) ICC Mouse
M Goolam, M Zernicka-G The chromatin modifier Satb1 regulates cell fate through Fgf signalling in the early mouse embryo Development, 2017;0(0):. 2017 [PMID: 28289135] (ICC,, Mouse) ICC Mouse
Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos Sci Rep, 2016;6(0):39457. 2016 [PMID: 27991575] (ICC,, Mouse) ICC Mouse
Abrogation Of Gap Junctional Communication In Es Cells Results In A Disruption Of Primitive Endoderm Formation In Embryoid Bodies Stem Cells, 2016;0(0):. 2016 [PMID: 27870307] (IHC Fresh,, Mouse) IHC Fresh Mouse
Asynchronous fate decisions by single cells collectively ensure consistent lineage composition in the mouse blastocyst Nat Commun, 2016;7(0):13463. 2016 [PMID: 27857135] (IHC Fresh,, Mouse) IHC Fresh Mouse
Show All 24 Publications.

Reviews for GATA-6 Antibody (AF1700) (6) 46

Average Rating: 4
(Based on 6 reviews)
We have 6 reviews tested in 1 species: Mouse.

Reviews using AF1700:
Filter by Applications
ICC
(1)
IHC-P
(2)
IF
(2)
ChIP
(1)
All Applications
Filter by Species
Mouse
(6)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry GATA-6 AF1700
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reviewed by:
Anonymous
ICC Mouse 08/04/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedR1 wild type Mouse embryonic Stem Cells
SpeciesMouse
LotKWT0214121

Blocking

Blocking DetailsBlocking: 0.1% Triton X in PBS with 5% donkey serum, 1 hr at RT

Primary Anitbody

Dilution Ratio1:200, 14 hrs, 4 C, Buffer: 0.1% Triton X + 5% donkey serum in PBS

Secondary Antibody

Secondary DescriptionDonkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 647 conjugate
Secondary Manufacturer Cat#A-21447
Secondary Concentration1:1000

Details

Detection NotesFluorescence Imaging, AF647 with cy5 filter cube, Nikon Eclipse Ti microscope, Exposure time: 1 sec
Fixation DetailsFixed for 10 mins with 4% formaldehyde at room temperature. Permeablization with 0.2% TritonX in PBS for 15 mins at RT
Wash DescriptionPBS,~1 min, 3 times

Comments

CommentsCells were cultured for a day in SerumLif before switching to endoderm differentiation conditions where Gata6 is expressed. Fixation after 3 days in differentiation conditions.
 
reviewed by:
Anonymous
IHC-P Mouse 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23002247
SpeciesMouse
 
reviewed by:
Anonymous
IHC-P Mouse 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22733991
SpeciesMouse
 
reviewed by:
Anonymous
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22733991
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22733991

Details

Detection NotesSee PMID 22733991

Comments

CommentsPublished: See PMID 22733991
 
reviewed by:
Anonymous
ChIP Mouse 01/05/2015
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedSee PMID 23002247
SpeciesMouse

Details

Detection NotesSee PMID 23002247

Comments

Comments"1ug of antibody per 500ug-1mg of chromatingood enrichment at target sites"See PMID 23002247
 
reviewed by:
Anonymous
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 20826529
SpeciesMouse

Blocking

Blocking DetailsSee PMID 20826529

Details

Detection NotesSee PMID 20826529

Comments

CommentsSamples were fixed in 4% formaldehyde and antibody was used at 1/200 dilution.Published: See PMID 20826529

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Secondary Antibodies

 

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Recent Reviews

4
5
1
4
4
3
1
2
0
1
0

Anonymous
08/04/2016
Application: ICC
Species: Mouse

 
Anonymous
01/05/2015
Application: IHC-P
Species: Mouse

 
Anonymous
01/05/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol GATA6
Entrez
Uniprot