GATA-6 Antibody [Unconjugated]

Western blot shows cytoplasmic and nuclear extracts from PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for GATA-6 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GATA-6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Goat Anti-Human GATA-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

KATO-III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA-6/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human GATA-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) or control antibody (AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. Themucin4promoter was detected by standard PCR.

GATA-6 was detected in immersion fixed paraffin-embedded sections of human intestine using Goat Anti-Human GATA-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western shows lysates of PC‑3 human prostate cancer cell line, loaded at 0.2 mg/ml. A specific band was detected for GATA‑6 at approximately 62 kDa (as indicated) using 10 µg/mL of Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

p38-Mapk14/11 inhibition during blastocyst maturation blocks PrE differentiation/maturation. (a) Experimental schema of p38-Mapk14/11 inhibition (+SB220025), plus vehicle control (+DMSO), and the details of antibodies used to analyse ICM cell lineage marker protein expression by immunofluorescence (IF) in late blastocysts (E4.5); Nanog and Gata4 (+DMSO n = 27, +SB220025 n = 33)—green, Nanog and Sox17 (+DMSO n = 18, +SB220025 n = 20)—red, and Nanog and Gata6 (+DMSO n = 24, +SB220025 n = 27)—blue. (b) Representative single confocal z-plane micrographs of vehicle control-treated (+DMSO) or p38-Mapk14/11 inhibited (+SB220025) late-blastocyst stage/equivalent embryos, immunofluorescently stained for indicated ICM cell lineage markers (Nanog in green and Gata4, Sox17 and Gata6 in red, plus DAPI DNA stain in blue). Examples of cells classified as TE, PrE and EPI are marked with an asterisk, arrowhead and arrow, respectively. Scale bar, 15 µm. (c) Pie charts of the relative cell lineage contribution in vehicle control (+DMSO) and p38-Mapk14/11 inhibited (+SB220025) blastocysts as judged by IF to detect the stated ICM lineage marker proteins. Blue, trophectoderm (TE); yellow, epiblast (EPI—ICM exhibiting exclusive Nanog expression); green, primitive endoderm (PrE—ICM exhibiting exclusive Gata4/6 or Sox17 expression, as appropriate); orange, uncommitted ICM cells (exhibiting co-expression of both Nanog and Gata4/6 or Sox17, as appropriate); and grey, ICM cells negative for either assayed marker. (d) Bar charts show average number of cells allocated to each specified ICM lineage, as judged by the indicated IF staining regime employed. Error bars represent s.e.m. and asterisks denote statistical significant differences in cell number between the vehicle control (+DMSO, black bars) and p38-Mapk14/11 inhibited (+SB220025, grey bars) embryo groups, according to two-tailed Student's t-test, with *p < 0.05 and **p < 0.005 confidence intervals. Yellow asterisk denotes increase in cells positively immunofluorescently staining for both EPI and PrE ICM markers using anti-Nanog and anti-Gata6 (an early PrE marker) antibodies. All individual embryo data used in the preparation of this figure are contained within the electronic supplementary material, table S3. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27605380), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reduced expression of the primitive endoderm markers Gata4 and Gata6 and increased expression of Nanog was observed in embryoid bodies following inhibitor of the Fgfr.Embryoid bodies were grown in different concentrations of AZD-4547 or 0.08% DMSO for 7 days. Expression levels of (A) Gata6, and (B) Gata4 were analysed using western blotting. A representative blot and quantification from 3 independent experiments is shown for each marker. A dose dependent decrease in expression of both proteins was observed. Statistical analysis is a one-way Anova with a Dunnett’s post-hoc test. Whole-mount immunostaining of (C) Gata6 and (D) Nanog after treatment of embryoid bodies with 4 µM AZD-4547 or 0.04% DMSO. A reduction in the percentage of nuclei expressing Gata6 was observed. The percentage of nuclei expressing Nanog increased. A representative image from 3 independent experiments is shown. Scale bars 10 µm. Dotted lines represent position that the relevant orthogonal or aerial images were taken. Statistical analysis is a paired t-test. Data is from 3 independent experiments, error bars represent SEM. (*P = 0.1–0.5, **p = 0.001–0.01, ***p<0.001). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0095434), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reduced expression of primitive endoderm markers Gata4 and Gata6 and increased expression of Nanog was observed in embryoid bodies upon inhibition of Mek.Embryoid bodies were grown in different concentrations of PD-0325901 or 0.04% DMSO for 7 days. Expression levels of (A) Gata6, and (B) Gata4 were analysed using western blotting. A representative blot and quantification from 3 independent experiments is shown for each marker. A dose dependent decrease in expression of both proteins was observed. Statistical analysis is a one-way Anova with a Dunnett’s post-hoc test. Whole-mount immunostaining of (C) Gata6 and (D) Nanog after treatment of embryoid bodies with 4 µM PD-0325901. A reduction in the percentage of nuclei expressing Gata6 was observed whilst there was an increase in the percentage of nuclei expressing Nanog. A representative image from 3 independent experiments is shown. Scale bars 10 µm. Dotted lines represent position that the relevant orthogonal or aerial images were taken. Statistical test is a paired t-test. Data is from 3 independent experiments, error bars represent SEM. (*P = 0.1–0.5, **p = 0.001–0.01, ***p<0.001). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0095434), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reduced expression of the primitive endoderm markers Gata4 and Gata6 and increased expression of Nanog was observed in embryoid bodies following inhibitor of the Fgfr.Embryoid bodies were grown in different concentrations of AZD-4547 or 0.08% DMSO for 7 days. Expression levels of (A) Gata6, and (B) Gata4 were analysed using western blotting. A representative blot and quantification from 3 independent experiments is shown for each marker. A dose dependent decrease in expression of both proteins was observed. Statistical analysis is a one-way Anova with a Dunnett’s post-hoc test. Whole-mount immunostaining of (C) Gata6 and (D) Nanog after treatment of embryoid bodies with 4 µM AZD-4547 or 0.04% DMSO. A reduction in the percentage of nuclei expressing Gata6 was observed. The percentage of nuclei expressing Nanog increased. A representative image from 3 independent experiments is shown. Scale bars 10 µm. Dotted lines represent position that the relevant orthogonal or aerial images were taken. Statistical analysis is a paired t-test. Data is from 3 independent experiments, error bars represent SEM. (*P = 0.1–0.5, **p = 0.001–0.01, ***p<0.001). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0095434), licensed under a CC-BY license. Not internally tested by R&D Systems.

expression of primitive endoderm cell fate markers gradually increased and nanog decreased in developing embryoid bodies.Embryoid bodies were produced from R63 mES cells using the hanging drop method. Development of the embryoid body was monitored over ten days. (A) Light microscopy images show the gradual increase in size of the embryoid bodies as well as increased heterogeneity, loss of circularity and formation of cystic cavities at later timepoints. Scale bars 200 µm. (B) Whole-mount immunostaining showing nuclear localisation of Gata6, Gata4, and Hnf4 alpha on days 3, 5, 7, and 10 of embryoid body development. The percentage of positive nuclei for each protein is shown graphically. The number of positive nuclei increased, reaching a maximum on day 7. (C) Whole-mount immunostaining showing nuclear localisation of Nanog. The number of positive nuclei rapidly decreased, with no positive cells seen on days 7 or 10. The percentage of positive nuclei for each protein is shown graphically. Data is from at least 3 independent experiments, error bars are standard error of the mean (SEM). Statistical analysis is a one-way Anova with a Tukey’s post-hoc test, (*P = 0.1–0.5, **p = 0.001–0.01, ***p<0.001). Dotted lines represent position that the relevant orthogonal or aerial images were taken. Scale bars 10 µm. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0095434), licensed under a CC-BY license. Not internally tested by R&D Systems.

Gata factor expression in embryoid bodies (EBs) at day 5.5 culture and in ex vivo visceral yolk sac (VYS) at E17.5 in relation to maternal diet. (A) Expression of Gata4, Gata6 and Dab2 mRNA in EBs of Emb-LPD and NPD groups presented as ratio to the geometric mean of Gapdh and Ppib transcripts (n = 5 per treatment). (B) Expression of Gata6 and Dab2 protein in EBs from NPD and Emb-LPD groups (n = 6 cell lines per treatment). Upper: representative images of protein immunoblot bands. Lower: band intensity normalized to alpha -tubulin expression. (C) Expression of Gata6 protein in VYS from Emb-LPD, LPD and NPD group (n = 4 samples per treatment). Upper: representative images of protein immunoblot bands. Lower: band intensity normalized to alpha -tubulin expression. Values presented are mean ± SEM. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//bmcdevbiol.biomedcentral.com/articles/10.1186/s12861-015-0053-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reduced expression of primitive endoderm markers Gata4 and Gata6 and increased expression of Nanog was observed in embryoid bodies upon inhibition of Mek.Embryoid bodies were grown in different concentrations of PD-0325901 or 0.04% DMSO for 7 days. Expression levels of (A) Gata6, and (B) Gata4 were analysed using western blotting. A representative blot and quantification from 3 independent experiments is shown for each marker. A dose dependent decrease in expression of both proteins was observed. Statistical analysis is a one-way Anova with a Dunnett’s post-hoc test. Whole-mount immunostaining of (C) Gata6 and (D) Nanog after treatment of embryoid bodies with 4 µM PD-0325901. A reduction in the percentage of nuclei expressing Gata6 was observed whilst there was an increase in the percentage of nuclei expressing Nanog. A representative image from 3 independent experiments is shown. Scale bars 10 µm. Dotted lines represent position that the relevant orthogonal or aerial images were taken. Statistical test is a paired t-test. Data is from 3 independent experiments, error bars represent SEM. (*P = 0.1–0.5, **p = 0.001–0.01, ***p<0.001). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0095434), licensed under a CC-BY license. Not internally tested by R&D Systems.

Gata factor expression in embryoid bodies (EBs) at day 5.5 culture and in ex vivo visceral yolk sac (VYS) at E17.5 in relation to maternal diet. (A) Expression of Gata4, Gata6 and Dab2 mRNA in EBs of Emb-LPD and NPD groups presented as ratio to the geometric mean of Gapdh and Ppib transcripts (n = 5 per treatment). (B) Expression of Gata6 and Dab2 protein in EBs from NPD and Emb-LPD groups (n = 6 cell lines per treatment). Upper: representative images of protein immunoblot bands. Lower: band intensity normalized to alpha -tubulin expression. (C) Expression of Gata6 protein in VYS from Emb-LPD, LPD and NPD group (n = 4 samples per treatment). Upper: representative images of protein immunoblot bands. Lower: band intensity normalized to alpha -tubulin expression. Values presented are mean ± SEM. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//bmcdevbiol.biomedcentral.com/articles/10.1186/s12861-015-0053-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ChIP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GATA-6 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human GATA-6
  Met1-Thr449
  Accession # Q92908
• Specificity: Detects human GATA-6 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GATA6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-6 Antibody [Unconjugated]

• GATA binding protein 6
• GATA6
• GATA-6
• GATA-binding factor 6
• GATA-binding protein 6
• transcription factor GATA-6

Background

GATA-6 (also named GATA-GT1) is a zinc finger transcription activating protein that bins to a T/A-G-A-T-A-G/A DNA sequence motif in mesodermally and endodermally-derived tissue. There are two GATA-6 isoforms that arise from alternate start sites: a short form of 449 amino acids (aa), and a long form of 595 aa. The short form of human GATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat and mouse, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GATA-6 Antibody (AF1700)(153)

Publications using AF1700

• Söderqvist, S;Viljamaa, A;Geyer, N;Keller, AL;Ruksha, K;Strell, C;Hekmati, N;Niculae, A;Engstrand, J;Sparrelid, E;Salmén, C;Costa, TDF;Zhao, M;Strömblad, S;Zacharouli, A;Ghorbani, P;Harrizi, S;Hamidi, Y;Khorosjutina, O;Milanova, S;Schmierer, B;Bozóky, B;Fernández Moro, C;Gerling, M; An injury-associated lobular microniche is associated with the classical tumor cell phenotype in pancreatic cancer Nature communications 2025-09-26 [PMID: 41006303] (Immunohistochemistry, Immunocytochemistry, Mouse, Transgenic Mouse)
• Liu, K;Yan, Z;Bai, D;Jiang, R;Bi, Y;Ma, X;Xiang, J;Sheng, Y;Dong, B;Ning, Z;Yi, S;Liu, Y;Lei, X;Jia, Y;Zhang, Y;Zhang, Y;Li, Y;Wu, T;Xi, C;Liu, S;Liu, S;Chen, J;Yin, J;Kou, X;Zhao, Y;Wang, H;Wang, Y;Wei, K;Gao, S;Liu, W; Modeling post-gastrula development via bidirectional pluripotent stem cells Cell research 2025-08-29 [PMID: 40883608] (Immunohistochemistry, Human)
• Godeau, AL;Seriola, A;Tchaicheeyan, O;Casals, M;Denkova, D;Aroca, E;Massafret, O;Parra, A;Demestre, M;Ferrer-Vaquer, A;Goren, S;Veiga, A;Solé, M;Boada, M;Comelles, J;Martínez, E;Colombelli, J;Lesman, A;Ojosnegros, S; Traction force and mechanosensitivity mediate species-specific implantation patterns in human and mouse embryos Science advances 2025-08-15 [PMID: 40815643] (Immunohistochemistry, Mouse)
• Ming, H;Scatolin, G;Ojeda-Rojas, O;Jiang, Z; Establishment of bovine extraembryonic endoderm stem cells enables efficient blastoid formation Cell Reports 2025-05-20 [PMID: 40402743] (Immunohistochemistry, Bovine)
• Nishida, H;Ando, K;Kaimori, R;Kawamura, K;Daa, T; Prognostic value of protein expression, tumor morphology and location within the pancreas in pancreatic ductal adenocarcinoma Oncology letters 2025-06-01 [PMID: 40264825] (Immunohistochemistry-Paraffin, Human)
• Moghe, P;Belousov, R;Ichikawa, T;Iwatani, C;Tsukiyama, T;Erzberger, A;Hiiragi, T; Coupling of cell shape, matrix and tissue dynamics ensures embryonic patterning robustness Nature cell biology 2025-02-18 [PMID: 39966670] (Immunohistochemistry, Transgenic Mouse)
• Ming, H;Scatolin, GN;Ojeda, A;Jiang, Z; Establishment of bovine extraembryonic endoderm cells bioRxiv : the preprint server for biology 2024-12-17 [PMID: 39763779] (Immunocytochemistry, Bovine)
• Nicoletti, C;Massenet, J;Pintado-Urbanc, AP;Connor, LJ;Nicolau, M;Sundar, S;Xu, M;Schmitt, A;Zhang, W;Fang, Z;Chan, TCI;Tapscott, SJ;Cheung, TH;Simon, MD;Caputo, L;Puri, PL; E-box independent chromatin recruitment turns MYOD into a transcriptional repressor bioRxiv : the preprint server for biology 2024-12-05 [PMID: 39677796] (Immunocytochemistry, Human)
• Pinzon-Arteaga, CA;O'Hara, R;Mazzagatti, A;Ballard, E;Hu, Y;Pan, A;Schmitz, DA;Wei, Y;Sakurai, M;Ly, P;Banaszynski, LA;Wu, J; TASOR expression in naive embryonic stem cells safeguards their developmental potential Cell reports 2024-10-24 [PMID: 39453814] (Immunohistochemistry, Human)
• McCreery, KP;Stubb, A;Stephens, R;Fursova, NA;Cook, A;Kruse, K;Michelbach, A;Biggs, LC;Keikhosravi, A;Nykänen, S;Hydén-Granskog, C;Zou, J;Lackmann, JW;Niessen, CM;Vuoristo, S;Miroshnikova, YA;Wickström, SA; Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells bioRxiv : the preprint server for biology 2024-09-07 [PMID: 39372762] (Immunohistochemistry, Immunocytochemistry, Human)
• Gutierrez-Sainz, L;Heredia-Soto, V;Rodríguez-García, AM;Crespo Sánchez, MG;Serrano-Olmedo, MG;Molero-Luis, M;Losantos-García, I;Ghanem, I;Pérez-Wert, P;Custodio, A;Mendiola, M;Feliu, J; Cytokines and Pancreatic Ductal Adenocarcinoma: Exploring Their Relationship with Molecular Subtypes and Prognosis International journal of molecular sciences 2024-08-29 [PMID: 39273323] (Immunohistochemistry, Human)
• Schumacher, S;Fernkorn, M;Marten, M;Chen, R;Kim, YS;Bedzhov, I;Schröter, C; Tissue-intrinsic beta-catenin signals antagonize Nodal-driven anterior visceral endoderm differentiation Nature communications 2024-06-13 [PMID: 38871742] (Immunocytochemistry, Human)
• Dattani, A;Corujo-Simon, E;Radley, A;Heydari, T;Taheriabkenar, Y;Carlisle, F;Lin, S;Liddle, C;Mill, J;Zandstra, PW;Nichols, J;Guo, G; Naive pluripotent stem cell-based models capture FGF-dependent human hypoblast lineage specification Cell stem cell 2024-05-24 [PMID: 38823388] (Immunohistochemistry, Human)
• Tan, JP;Liu, X;Polo, JM; Reprogramming fibroblast into human iBlastoids Nature protocols 2024-04-17 [PMID: 38632379] (Immunocytochemistry, Human)
• Weatherbee, BAT;Weberling, A;Gantner, CW;Iwamoto-Stohl, LK;Barnikel, Z;Barrie, A;Campbell, A;Cunningham, P;Drezet, C;Efstathiou, P;Fishel, S;Vindel, SG;Lockwood, M;Oakley, R;Pretty, C;Chowdhury, N;Richardson, L;Mania, A;Weavers, L;Christie, L;Elder, K;Snell, P;Zernicka-Goetz, M; Distinct pathways drive anterior hypoblast specification in the implanting human embryo Nature cell biology 2024-03-01 [PMID: 38443567] (Immunohistochemistry, Human, Mouse)
• Khoa, LTP;Yang, W;Shan, M;Zhang, L;Mao, F;Zhou, B;Li, Q;Malcore, R;Harris, C;Zhao, L;Rao, R;Iwase, S;Kalantry, S;Bielas, SL;Lyssiotis, CA;Dou, Y; Quiescence enables unrestricted cell fate in naive embryonic stem cells Nature communications 2024-02-26 [PMID: 38409226] (Immunocytochemistry, Mouse)
• Kruger, RE;Frum, T;Brumm, AS;Hickey, SL;Niakan, KK;Aziz, F;Shammami, MA;Roberts, JG;Ralston, A; Smad4 is essential for epiblast scaling and morphogenesis after implantation, but nonessential prior to implantation in the mouse bioRxiv : the preprint server for biology 2024-01-24 [PMID: 38328075] (Immunohistochemistry, Mouse)
• Chousal, JN;Morey, R;Srinivasan, S;Lee, K;Zhang, W;Yeo, AL;To, C;Cho, K;Garzo, VG;Parast, MM;Laurent, LC;Cook-Andersen, H; Molecular profiling of human blastocysts reveals primitive endoderm defects among embryos of decreased implantation potential Cell reports 2024-01-25 [PMID: 38277271] (Immunohistochemistry, Human)
• Yu, L;Logsdon, D;Pinzon-Arteaga, CA;Duan, J;Ezashi, T;Wei, Y;Ribeiro Orsi, AE;Oura, S;Liu, L;Wang, L;Liu, K;Ding, X;Zhan, L;Zhang, J;Nahar, A;Stobbe, C;Katz-Jaffe, M;Schoolcraft, WB;Tan, T;Hon, GC;Yuan, Y;Wu, J; Large-scale production of human blastoids amenable to modeling blastocyst development and maternal-fetal cross talk Cell stem cell 2023-09-07 [PMID: 37683605] (Immunocytochemistry, Human)
• Karvas, RM;Zemke, JE;Ali, SS;Upton, E;Sane, E;Fischer, LA;Dong, C;Park, KM;Wang, F;Park, K;Hao, S;Chew, B;Meyer, B;Zhou, C;Dietmann, S;Theunissen, TW; 3D-cultured blastoids model human embryogenesis from pre-implantation to early gastrulation stages Cell stem cell 2023-09-07 [PMID: 37683602] (Immunocytochemistry, Human)
• Marina Gattiglio, Michelle Protzek, Christian Schröter Population-level antagonism between FGF and BMP signaling steers mesoderm differentiation in embryonic stem cells Biology Open 8/15/2023 [PMID: 37530863]
• Goissis, MD;Bradshaw, B;Posfai, E;Rossant, J; Influence of FGF4 and BMP4 on FGFR2 dynamics during the segregation of epiblast and primitive endoderm cells in the pre-implantation mouse embryo PloS one 2023-07-20 [PMID: 37471320] (IHC, Mouse)
• Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelse, Sanne Hillenius, Talia Van Der Helm et al. Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay Cell Reports Methods 6/26/2023 [PMID: 37426764]
• Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelse, Sanne Hillenius, Talia Van Der Helm et al. Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay Cell Reports Methods 2023-06-26 [PMID: 37426764] (Immunocytochemistry, Human)
• Toyooka, Y;Aoki, K;Usami, FM;Oka, S;Kato, A;Fujimori, T; Generation of pulsatile ERK activity in mouse embryonic stem cells is regulated by Raf activity Scientific reports 2023-06-10 [PMID: 37301878] (ICC, Transgenic Mouse)
• Kuisheng Liu, Xiaocui Xu, Dandan Bai, Yanhe Li, Yalin Zhang, Yanping Jia et al. Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm Protein & Cell 4/21/2023 [PMID: 37084236]
• Kuisheng Liu, Xiaocui Xu, Dandan Bai, Yanhe Li, Yalin Zhang, Yanping Jia et al. Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm Protein & Cell 2023-04-21 [PMID: 37084236] (Western Blot, Immunocytochemistry, Mouse)
• V Garg, Y Yang, S Nowotschin, M Setty, YY Kuo, R Sharma, A Polyzos, E Salataj, D Murphy, A Jang, D Pe'er, E Apostolou, AK Hadjantona Single-cell analysis of bidirectional reprogramming between early embryonic states reveals mechanisms of differential lineage plasticities bioRxiv : the preprint server for biology, 2023-03-29;0(0):. 2023-03-29 [PMID: 37034770] (ChIP, Flow Cytometry, ICC, Mouse)
• W Zhang, M Wang, Z Song, Q Fu, J Chen, W Zhang, S Gao, X Sun, G Yang, Q Zhang, J Yang, H Tang, H Wang, X Kou, H Wang, Z Mao, X Xu, S Gao, Y Jiang Farrerol directly activates the deubiqutinase UCHL3 to promote DNA repair and reprogramming when mediated by somatic cell nuclear transfer Nature Communications, 2023-04-03;14(1):1838. 4/3/2023 [PMID: 37012254]
• P Athanasoul, M Balli, A De Jaime-S, A Boel, S Papanikola, BK van der Ve, A Janiszewsk, T Vanhessche, A Francis, Y El Laithy, AL Nigro, F Aulicino, KP Koh, V Pasque, MP Cosma, C Verfaillie, A Zwijsen, B Heindryckx, C Nikolaou, F Lluis The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Nature Communications, 2023-03-03;14(1):1210. 2023-03-03 [PMID: 36869101] (ICC, Mouse)
• Victoria Heredia-Soto, Laura Gutiérrez-Sainz, Ismael Ghanem, Laura Guerra, Elena Palacios, Marta de Uribe et al. The Relationship between the Expression of GATA4 and GATA6 with the Clinical Characteristics and Prognosis of Resectable Pancreatic Adenocarcinoma Biomedicines 1/18/2023 [PMID: 36830789]
• C Dupont, OJM Schäffers, BF Tan, S Merzouk, EM Bindels, A Zwijsen, D Huylebroec, J Gribnau Efficient generation of ETX embryoids that recapitulate the entire window of murine egg cylinder development Science Advances, 2023-01-18;9(3):eadd2913. 2023-01-18 [PMID: 36652512] (IHC, Mouse)
• Zachary T. Olmsted, Maria Belen Paredes-Espinosa, Janet L. Paluh Generation of human elongating multi-lineage organized cardiac gastruloids STAR Protocols 12/16/2022 [PMID: 36595961]
• D Li, J Yang, V Malik, Y Huang, X Huang, H Zhou, J Wang An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity Nucleic Acids Research, 2022-11-28;0(0):. 2022-11-28 [PMID: 36416264] (ICC, Mouse)
• Vera Zywitza, Silke Frahm, Norman Krüger, Anja Weise, Frank Göritz, Robert Hermes et al. Induced pluripotent stem cells and cerebral organoids from the critically endangered Sumatran rhinoceros iScience 11/18/2022 [PMID: 36388963]
• Samuel Hess, Timothy J. Kendall, Maria Pena, Keitaro Yamane, Daniel Soong, Linda Adams et al. In vivo partial reprogramming by bacteria promotes adult liver organ growth without fibrosis and tumorigenesis Cell Reports Medicine 11/15/2022 [PMID: 36384103]
• Chousal JN, Sohni A, Vitting-Seerup K et al. Progression of the pluripotent epiblast depends upon the NMD Factor UPF2 Development (Cambridge, England) 10/18/2022 [PMID: 36255229]
  
  Details:
  Dilution used in ICC 1:1000
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  Details:
  DNMT3B Antibody (clone 52A1018; Imgenex IMG-184A) was used for immunofluorescence staining of the murine whole embryos which were fixed at in 4% formaldehyde for 30?minutes at room temperature and permeabilized in 0.5% Tween20 & Triton X100. The antibody was used at 1:150 dilution (see full text for detailed protocol).
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Reviews for GATA-6 Antibody (AF1700) (6)

Average Rating: 4

(Based on 6 reviews)

We have 6 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer ICC Mouse 08/04/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: R1 wild type Mouse embryonic Stem Cells
• Species: Mouse
• Lot: KWT0214121

reviewed by: Verified Customer IHC-P Mouse 01/05/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 23002247
• Species: Mouse

reviewed by: Verified Customer IHC-P Mouse 01/05/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 22733991
• Species: Mouse

reviewed by: Verified Customer IF Mouse 01/05/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22733991
• Species: Mouse

reviewed by: Verified Customer ChIP Mouse 01/05/2015

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: See PMID 23002247
• Species: Mouse

reviewed by: Verified Customer IF Mouse 01/05/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 20826529
• Species: Mouse

Bioinformatics

• Gene Symbol: GATA6
• Uniprot:
  • Q92908()