GATA-6 Antibody [Unconjugated]

Images

 
Western blot shows cytoplasmic and nuclear extracts from PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody ...read more
KATO‑III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA‑6/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human ...read more
GATA‑6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) for 3 hours at room ...read more
GATA‑6 was detected in immersion fixed paraffin-embedded sections of human intestine using Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) at 5 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

GATA-6 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GATA-6
Met1-Thr449
Accession # Q92908
Specificity
Detects human GATA-6 in direct ELISAs and Western blots.  In direct ELISAs, approximately 5% cross-reactivity with recombinant human GATA-5 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GATA6
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 6 Reviews rated 4
using
AF1700 in the following applications:

Publications
Read Publications using
AF1700 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-6 Antibody [Unconjugated]

  • GATA binding protein 6
  • GATA6
  • GATA-6
  • GATA-binding factor 6
  • GATA-binding protein 6
  • transcription factor GATA-6

Background

GATA-6 (also named GATA-GT1) is a zinc finger transcription activating protein that bins to a T/A-G-A-T-A-G/A DNA sequence motif in mesodermally and endodermally-derived tissue. There are two GATA-6 isoforms that arise from alternate start sites: a short form of 449 amino acids (aa), and a long form of 595 aa. The short form of human GATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat and mouse, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-24585
Species: Hu, Mu
Applications: IHC, IHC-P, WB
AF1700
Species: Hu
Applications: ChIP, ICC, IHC, WB
NBP1-87992
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-24585
Species: Hu, Mu
Applications: IHC, IHC-P, WB
AF2170
Species: Hu
Applications: ChIP, ICC, WB
AF1759
Species: Hu, Mu
Applications: ChIP, ICC, IP, Simple Western, WB
AF1997
Species: Hu
Applications: ChIP, ICC, IHC, WB
H00388112-B01P
Species: Hu
Applications: WB
NBP2-24583
Species: Bv, Eq, Hu, Mu, Ma-Op, Pm, Rt
Applications: IHC, IHC-P, WB
NBP2-88921
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-44501
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IF, IHC, IHC-P
314-BP/CF
Species: Hu
Applications: BA, BA
AF2444
Species: Hu
Applications: IHC, WB
MAB1368
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow, KO, Simple Western, WB
MAB2018
Species: Hu, Mu, Rt
Applications: CyTOF-reported, ICC, ICFlow, Simple Western, WB
AF2400
Species: Hu
Applications: ChIP, ICC, IHC, WB
7734-LF
Species: Hu
Applications: BA
AF2605
Species: Hu
Applications: ICC, IHC, Simple Western, WB
AF2170
Species: Hu
Applications: ChIP, ICC, WB
AF1700
Species: Hu
Applications: WB, IHC, ChIP, ICC/IF

Publications for GATA-6 Antibody (AF1700)(49)

We have publications tested in 5 confirmed species: Human, Mouse, Bovine, Cynomolgus Monkey, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 9 applications: ChIP, Flow Cytometry, ICC, ICC/IF, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(3)
Flow Cytometry
(4)
ICC
(21)
ICC/IF
(1)
IHC
(9)
IHC-Fr
(3)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(13)
Mouse
(32)
Bovine
(1)
Cynomolgus Monkey
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using AF1700 Applications Species
V Trappetti, J Fazzari, C Fernandez-, L Smyth, M Potez, N Shintani, B de Breuyn, OA Martin, V Djonov Targeted Accumulation of Macrophages Induced by Microbeam Irradiation in a Tissue-Dependent Manner Biomedicines, 2022;10(4):. 2022 [PMID: 35453485] (IHC, Mouse) IHC Mouse
ZY Zuo, GH Yang, HY Wang, SY Liu, YJ Zhang, Y Cai, F Chen, H Dai, Y Xiao, MB Cheng, Y Huang, Y Zhang Klf4 methylated by Prmt1 restrains the commitment of primitive endoderm Nucleic Acids Research, 2022;0(0):. 2022 [PMID: 35137179] (ICC, Mouse) ICC Mouse
N Rodina, A Khudiakov, K Perepelina, A Muravyev, A Boytsov, A Zlotina, P Sokolnikov, A Kostareva Generation of iPSC line (FAMRCi009-A) from patient with familial progressive cardiac conduction disorder carrying genetic variant FLNC p.Val2264Met Stem Cell Research, 2021;59(0):102640. 2021 [PMID: 34971933] (Flow Cytometry, Human) Flow Cytometry Human
K Perepelina, A Khudiakov, N Rodina, A Boytsov, T Vavilova, A Zlotina, P Sokolnikov, A Kostareva Generation of iPSC line FAMRCi010-A from patient with restrictive cardiomyopathy carrying genetic variant FLNC p.Gly2011Arg Stem Cell Research, 2021;59(0):102639. 2021 [PMID: 34971931] (Flow Cytometry, Human) Flow Cytometry Human
K Duan, CY Si, SM Zhao, ZY Ai, BH Niu, Y Yin, LF Xiang, H Ding, Y Zheng The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey Cells, 2021;10(10):. 2021 [PMID: 34685690] (ICC/IF, Cynomolgus Monkey) ICC/IF Cynomolgus Monkey
T Pachano, V Sánchez-Ga, T Ealo, M Mariner-Fa, T Bleckwehl, HG Asenjo, P Respuela, S Cruz-Molin, M Muñoz-San, E Haro, WFJ van IJcken, D Landeira, A Rada-Igles Orphan CpG islands amplify poised enhancer regulatory activity and determine target gene responsiveness Nature Genetics, 2021;53(7):1036-1049. 2021 [PMID: 34183853] (IHC, Mouse) IHC Mouse
A Zhao, H Qin, M Sun, M Tang, J Mei, K Ma, X Fu Chemical conversion of human epidermal stem cells into intestinal goblet cells for modeling mucus-microbe interaction and therapy Science Advances, 2021;7(16):. 2021 [PMID: 33853767] (ICC, Human) ICC Human
HS Lee, E Kim, J Lee, SJ Park, HK Hwang, CH Park, SY Jo, CM Kang, SM Hong, H Kang, JH Jo, IR Cho, MJ Chung, JY Park, SW Park, SY Song, JM Han, S Kim, S Bang Profiling of conditionally reprogrammed cell lines for in vitro chemotherapy response prediction of pancreatic cancer EBioMedicine, 2021;65(0):103218. 2021 [PMID: 33639403] (IHC, Human) IHC Human
X Wang, Y Xiang, Y Yu, R Wang, Y Zhang, Q Xu, H Sun, ZA Zhao, X Jiang, X Wang, X Lu, D Qin, Y Quan, J Zhang, N Shyh-Chang, H Wang, N Jing, W Xie, L Li Formative pluripotent stem cells show features of epiblast cells poised for gastrulation Cell Research, 2021;0(0):. 2021 [PMID: 33608671] (ICC, Mouse) ICC Mouse
A Weberling, M Zernicka-G Trophectoderm mechanics direct epiblast shape upon embryo implantation Cell Reports, 2021;34(3):108655. 2021 [PMID: 33472064] (IHC, Mouse) IHC Mouse
Show All 49 Publications.

Reviews for GATA-6 Antibody (AF1700) (6) 46

Average Rating: 4
(Based on 6 reviews)
We have 6 reviews tested in 1 species: Mouse.

Reviews using AF1700:
Filter by Applications
Immunocytochemistry
(1)
Immunohistochemistry-Paraffin
(2)
Immunofluorescence
(2)
Chromatin Immunoprecipitation
(1)
All Applications
Filter by Species
Mouse
(6)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry GATA-6 AF1700
Enlarge
4
reviewed by:
Simon Hastreiter
Immunocytochemistry Mouse 08/04/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedR1 wild type Mouse embryonic Stem Cells
SpeciesMouse
LotKWT0214121
  5
reviewed by:
Anonymous -
Immunohistochemistry-Paraffin Mouse 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23002247
SpeciesMouse
  4
reviewed by:
Anonymous -
Immunohistochemistry-Paraffin Mouse 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22733991
SpeciesMouse
  4
reviewed by:
Anonymous -
Immunofluorescence Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22733991
SpeciesMouse
  4
reviewed by:
Anonymous -
Chromatin Immunoprecipitation Mouse 01/05/2015
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedSee PMID 23002247
SpeciesMouse
  3
reviewed by:
Anonymous -
Immunofluorescence Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 20826529
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GATA-6 Antibody (AF1700) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional GATA-6 Products

Bioinformatics Tool for GATA-6 Antibody (AF1700)

Discover related pathways, diseases and genes to GATA-6 Antibody (AF1700). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GATA-6 Antibody (AF1700)

Discover more about diseases related to GATA-6 Antibody (AF1700).
 

Pathways for GATA-6 Antibody (AF1700)

View related products by pathway.

PTMs for GATA-6 Antibody (AF1700)

Learn more about PTMs related to GATA-6 Antibody (AF1700).

Blogs on GATA-6

There are no specific blogs for GATA-6, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
1
4
4
3
1
2
0
1
0

Simon Hastreiter
08/04/2016
Application: Immunocytochemistry
Species: Mouse

 
Anonymous -
01/05/2015
Application: Immunohistochemistry-Paraffin
Species: Mouse

 
Anonymous -
01/05/2015
Application: Immunohistochemistry-Paraffin
Species: Mouse

Bioinformatics

Gene Symbol GATA6
Uniprot