GATA-5 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-HumanGATA‑5 Antigen Affinity-purified ...read more
HeLa human cervical epithelial carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA‑5/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human ...read more
GATA‑5 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human GATA‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2170) at 1.7 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

GATA-5 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GATA-5
Met1-Ala397
Accession # Q9BWX5
Specificity
Detects human GATA-5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) GATA-1, rhGATA-2, rhGATA-3, rhGATA-4, and rhGATA-6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GATA5
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF2170 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-5 Antibody [Unconjugated]

  • bB379O24.1
  • GATA binding factor-5
  • GATA binding protein 5
  • GATA5
  • GATA-5
  • GATA-binding factor 5
  • GATA-binding protein 5
  • transcription factor GATA-5

Background

GATA-5 belongs to the GATA family of transcription factors which bind to the consensus DNA sequence (AIT) GATA (AIG) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA5 is important in the development and differentiation of endoderm-derived organs, including heart and gastrointestinal tissues. GATA-5 has also been implicated in cancer development (1, 2).
  1. Tsai, S.F. et al. (1989) Nature 339:446.
  2. Jiang, Y. and T. Evans (1996) Dev. Biol. 174:258.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GATA-5 Antibody (AF2170)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ChIP.


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(1)
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GATA5
Uniprot