NKX2.5 Antibody

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

NKX2.5 Antibody Summary

• Immunogen: E. coli-derived recombinant human NKX2.5
  Gln24-Arg137
  Accession # P52952
• Specificity: Detects human NKX2.5 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NKX2-5
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKX2.5 Antibody

• cardiac-specific homeo box
• cardiac-specific homeobox 1
• Cardiac-specific homeobox
• CHNG5
• CSX1
• CSXFLJ97195
• FLJ52202
• FLJ99536
• Homeobox protein CSX
• Homeobox protein NK-2 homolog E
• homeobox protein Nkx-2.5
• NK2 transcription factor related, locus 5 (Drosophila)
• NKX2.5
• NKX2.5FLJ97166
• NKX2-5
• NKX2E
• NKX2EFLJ97197
• NKX4-1
• tinman paralog

Background

The homeobox transcriptional factor NKX2.5 (Homeobox protein NK-2 homolog E) specifies cardiac and visceral mesoderm and plays an essential role in heart development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKX2.5 Antibody (AF2444)(5)

Publications using AF2444

• W Chen, D Pretorius, Y Zhou, Y Nakada, J Yang, J Zhang TT-10-loaded nanoparticles promote cardiomyocyte proliferation and cardiac repair in a mouse model of myocardial infarction JCI Insight, 2021;6(20):. 2021 [PMID: 34676829] (ICC, Mouse)
• Inhibiting Extracellular Vesicle Release from Human Cardiosphere Derived Cells with Lentiviral Knockdown of nSMase2 Differentially Effects Proliferation and Apoptosis in Cardiomyocytes, Fibroblasts and Endothelial Cells In Vitro PLoS ONE, 2016;11(11):e0165926. 2016 [PMID: 27806113] (IHC-Fr, Mouse)
• Luo J, Weaver M, Cao B, Dennis J, Van Biber B, Laflamme M, Allen M Cobalt protoporphyrin pretreatment protects human embryonic stem cell-derived cardiomyocytes from hypoxia/reoxygenation injury in vitro and increases graft size and vascularization in vivo. Stem Cells Transl Med, 2014;3(6):734-44. 2014 [PMID: 24736402] (IHC, Human)
• Mahmoud , Ahmed I, Porrello , Enzo R, Kimura , Wataru, Olson , Eric N, Sadek , Hesham A Surgical models for cardiac regeneration in neonatal mice. Nat Protoc, 2014;9(2):305-11. 2014 [PMID: 24434799] (IHC-P, Mouse)
• Stevens KR, Pabon L, Muskheli V Scaffold-free human cardiac tissue patch created from embryonic stem cells. Tissue Eng Part A, 2009;15(6):1211-22. 2009 [PMID: 19063661] (ICC, Human)

Reviews for NKX2.5 Antibody (AF2444) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Other.

reviewed by: Anonymous - IF Mouse 01/07/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 24123507
• Species: Mouse

reviewed by: Anonymous - IF Other 01/06/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 24123507
• Species: Other

Bioinformatics

• Gene Symbol: NKX2-5
• Uniprot:
  • P52952()