NKX2.5 Antibody

Images

 
There are currently no images for NKX2.5 Antibody (AF2444).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

NKX2.5 Antibody Summary

Immunogen
E. coli-derived recombinant human NKX2.5
Gln24-Arg137
Accession # P52952
Specificity
Detects human NKX2.5 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NKX2-5
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF2444 in the following applications:

Publications
Read Publications using
AF2444 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKX2.5 Antibody

  • cardiac-specific homeo box
  • cardiac-specific homeobox 1
  • Cardiac-specific homeobox
  • CHNG5
  • CSX1
  • CSXFLJ97195
  • FLJ52202
  • FLJ99536
  • Homeobox protein CSX
  • Homeobox protein NK-2 homolog E
  • homeobox protein Nkx-2.5
  • NK2 transcription factor related, locus 5 (Drosophila)
  • NKX2.5
  • NKX2.5FLJ97166
  • NKX2-5
  • NKX2E
  • NKX2EFLJ97197
  • NKX4-1
  • tinman paralog

Background

The homeobox transcriptional factor NKX2.5 (Homeobox protein NK-2 homolog E) specifies cardiac and visceral mesoderm and plays an essential role in heart development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-24585
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-24585
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-00493
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-83237
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-14873
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-71927
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF1837
Species: Hu
Applications: WB, Simple Western, ICC
NBP1-89090
Species: Hu
Applications: IHC, IHC-P
NB100-2379
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, ICC/IF, IHC
AF3876
Species: Hu, Mu
Applications: WB, IHC
NBP2-46076
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-44501
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IF
NBP1-46568
Species: Mu, Rt
Applications: WB, IHC, IHC-P
MAB4028
Species: Hu, Mu
Applications: WB

Publications for NKX2.5 Antibody (AF2444)(4)

Reviews for NKX2.5 Antibody (AF2444) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.

Reviews using AF2444:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
IF Mouse 01/07/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24123507
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24123507

Details

Detection NotesSee PMID 24123507

Comments

CommentsPublished: See PMID 24123507The Goat anti-Nkx2.5 polyclonal antibody was used to detect the homeobox transcription factor in the developing cardiac mesoderm during mouse embryogenesis. The antibody detected Nkx2.5 in whole mount mouse embryo samples, at embryonic day 7.75.
  4
reviewed by:
Anonymous
IF Other 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24123507
SpeciesOther

Blocking

Blocking DetailsSee PMID 24123507

Details

Detection NotesSee PMID 24123507

Comments

CommentsGoat anti-Nkx2.5 IgG, 100 ug/ml, dilution 1:250, mouse embryo whole-mount stainingPublished: See PMID 24123507

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NKX2.5 Antibody (AF2444) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF2444

Additional NKX2.5 Products

Bioinformatics Tool for NKX2.5 Antibody (AF2444)

Discover related pathways, diseases and genes to NKX2.5 Antibody (AF2444). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NKX2.5 Antibody (AF2444)

Discover more about diseases related to NKX2.5 Antibody (AF2444).
 

Pathways for NKX2.5 Antibody (AF2444)

View related products by pathway.

PTMs for NKX2.5 Antibody (AF2444)

Learn more about PTMs related to NKX2.5 Antibody (AF2444).

Blogs on NKX2.5

There are no specific blogs for NKX2.5, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Anonymous
01/07/2015
Application: IF
Species: Mouse

 
Anonymous
01/06/2015
Application: IF
Species: Other

Bioinformatics

Gene Symbol NKX2-5
Uniprot