Western Blot: GATA-2 Antibody [NBP1-82581] - Analysis in human cell line SH-SY5Y.
Immunocytochemistry/ Immunofluorescence: GATA-2 Antibody [NBP1-82581] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: GATA-2 Antibody [NBP1-82581] - Staining in human prostate and liver tissues. Corresponding GATA2 RNA-seq data are presented for the same tissues.
Western Blot: GATA-2 Antibody [NBP1-82581] - Analysis in human cell line HELA.
Immunohistochemistry-Paraffin: GATA-2 Antibody [NBP1-82581] - Staining of human kidney shows nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: GATA-2 Antibody [NBP1-82581] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: GATA-2 Antibody [NBP1-82581] - Staining of human placenta shows nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: GATA-2 Antibody [NBP1-82581] - Staining of human prostate shows nuclear positivity in glandular cells.
Simple Western: GATA-2 Antibody [NBP1-82581] - Simple Western lane view shows a specific band for GATA2 in 0.2 mg/ml of HEL lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: GATA-2 Antibody [NBP1-82581] - Electropherogram image(s) of corresponding Simple Western lane view. GATA-2 antibody was used at 1:100 dilution on HELA lysate(s).
This antibody was developed against Recombinant Protein corresponding to amino acids: LTGGQMCRPHLLHSPGLPWLDGGKAALSAAAAHHHNPWTVSPFSKTPLHPSAAGGPGGPLSVYPGAGGGSGGGSGSSVASLTPTAAHSGSHLFGFPPTPPKEVSPDPSTTGAASPASSSAGGSAARG
Specificity of human, mouse GATA-2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Rat (96%). Backed by our 100% Guarantee.
Immunogen affinity purified
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For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24583263).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
51 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Chiang YT. Identification of metastasis-driving genes as potential therapeutic targets/biomarkers for metastatic prostate cancer (Thesis Supervisor - Dr. Yuzhuo Wang, BC Cancer Agency Research Centre, Canada) Thesis 2015 (IHC-P, WB, ICC/IF, Human)
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