LMO2 Antibody (1A9-3B11) - BSA Free

Images

 
Western Blot: LMO2 Antibody (1A9-3B11) [NB110-78626] - Detection of LMO2 in Ramos cell lysate.
Immunohistochemistry-Paraffin: LMO2 Antibody (1A9-3B11) [NB110-78626] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using LMO2 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The ...read more
Flow Cytometry: LMO2 Antibody (1A9-3B11) [NB110-78626] - An intracellular stain was performed on Ramos cells with LMO2 Antibody (1A9-3B11) NB110-78626 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Flow Cytometry: LMO2 Antibody (1A9-3B11) [NB110-78626] - An intracellular stain was performed on THP-1 cells with LMO2 Antibody (1A9-3B11) NB110-78626 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Immunofluorescence: LMO2 Antibody (1A9-3B11) [NB110-78626] - Immunofluorescent labeling of LMO2 (green) in tonsil tissue.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, Flow, IHC, IHC-P, IP, ICC/IF
Clone
1A9-3B11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

LMO2 Antibody (1A9-3B11) - BSA Free Summary

Immunogen
Recombinant human LMO2. [UniProt# P25791]
Localization
Nuclear.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LMO2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10-1:500
  • Flow Cytometry 1-2 ug/ml per million cells
  • Immunofluorescence
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 0.5 ug/ml
Application Notes
This LMO2 antibody is useful for Western blot, Immunoprecipitation, Immunofluorescence on tissue, Chromatin Immunoprecipitation and Immunohistochemistry on paraffin-embedded sections. In WB a band can be seen at ~24 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB110-78626 in the following applications:

  • 1 publication
  • 1 publication
  • WB
    2 publications

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34382935). .

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for LMO2 Antibody (1A9-3B11) - BSA Free

  • Cysteine-rich protein TTG-2
  • LIM domain only 2 (rhombotin-like 1)
  • LIM domain only protein 2
  • LMO2
  • LMO-2
  • RBTN2
  • RBTN2rhombotin-2
  • RBTNL1
  • RBTNL1rhombotin-like 1
  • RHOM2
  • RHOM2T-cell translocation protein 2
  • T-cell translocation gene 2
  • TTG2
  • TTG2rhombotin 2

Background

LMO2 (LIM-only protein 2) is a nuclear transcriptional regulator essential for normal blood cell development and it exerts its effects by mediating protein-protein interactions as well as by nucleating multicomponent transcriptional complexes. LMO2 interacts with LDB1 (LIM domain binding protein 1) through the tandem LIM domains of LMO2 and LID (LIM interaction domain) of LDB1. LMO2 also interacts with bHlH proteins TAL1/SCL, BEX2 and KDM5A, and form complex with TAL1/SCL. It functions with TAL1/SCL in the regulation of RBCs development and with LDB1 to maintain erythroid precursors in immature state. LMO2 has been associated with the maintenance of hematopoietic stem cells, RBCs differentiation, and angiogenesis in both normal development as well as during carcinogenesis. Disruption of LMO2 in mice causes death in midway through embryonic development as they do not develop RBCs. Excessive expression of LMO2 in T-cells, through either chromosomal translocations or retroviral insertion of gene therapy vectors, as well as transgenic overexpression of LMO2 in mouse model leads to the onset of T-cell acute lymphoblastic leukemia (T-ALL).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LMO2 Antibody (NB110-78626)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ChIP, IHC-P, WB.


Filter By Application
ChIP
(1)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LMO2