LMO2 Antibody (1A9-3B11) - BSA Free

Western Blot: LMO2 Antibody (1A9-3B11) [NB110-78626] - Detection of LMO2 in Ramos cell lysate.

Immunohistochemistry-Paraffin: LMO2 Antibody (1A9-3B11) [NB110-78626] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using LMO2 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear with some cytoplasmic staining was observed. Staining was performed by Histowiz.

Flow Cytometry: LMO2 Antibody (1A9-3B11) [NB110-78626] - An intracellular stain was performed on Ramos cells with LMO2 Antibody (1A9-3B11) NB110-78626 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Flow Cytometry: LMO2 Antibody (1A9-3B11) [NB110-78626] - An intracellular stain was performed on THP-1 cells with LMO2 Antibody (1A9-3B11) NB110-78626 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Immunohistochemistry: LMO2 Antibody (1A9-3B11) - BSA Free [NB110-78626] - Immunohistochemistry labeling of LMO2 (green) in tonsil tissue.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC, IP, ChIP, ICC/IF (-)
• Clone: 1A9-3B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

LMO2 Antibody (1A9-3B11) - BSA Free Summary

• Immunogen: Recombinant human LMO2. [UniProt# P25791]
• Localization: Nuclear.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LMO2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Flow Cytometry 1-2 ug/ml per million cells
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 0.5 ug/ml
• Application Notes: This LMO2 antibody is useful for Western blot, Immunoprecipitation, Chromatin Immunoprecipitation and Immunohistochemistry on paraffin-embedded sections. In WB a band can be seen at ~24 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34382935).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for LMO2 Antibody (1A9-3B11) - BSA Free

• Cysteine-rich protein TTG-2
• LIM domain only 2 (rhombotin-like 1)
• LIM domain only protein 2
• LMO2
• LMO-2
• RBTN2
• RBTN2rhombotin-2
• RBTNL1
• RBTNL1rhombotin-like 1
• RHOM2
• RHOM2T-cell translocation protein 2
• T-cell translocation gene 2
• TTG2
• TTG2rhombotin 2

Background

LMO2 (LIM-only protein 2) is a nuclear transcriptional regulator essential for normal blood cell development and it exerts its effects by mediating protein-protein interactions as well as by nucleating multicomponent transcriptional complexes. LMO2 interacts with LDB1 (LIM domain binding protein 1) through the tandem LIM domains of LMO2 and LID (LIM interaction domain) of LDB1. LMO2 also interacts with bHlH proteins TAL1/SCL, BEX2 and KDM5A, and form complex with TAL1/SCL. It functions with TAL1/SCL in the regulation of RBCs development and with LDB1 to maintain erythroid precursors in immature state. LMO2 has been associated with the maintenance of hematopoietic stem cells, RBCs differentiation, and angiogenesis in both normal development as well as during carcinogenesis. Disruption of LMO2 in mice causes death in midway through embryonic development as they do not develop RBCs. Excessive expression of LMO2 in T-cells, through either chromosomal translocations or retroviral insertion of gene therapy vectors, as well as transgenic overexpression of LMO2 in mouse model leads to the onset of T-cell acute lymphoblastic leukemia (T-ALL).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LMO2 Antibody (NB110-78626)(5)

Publications using NB110-78626

• Seo S, Hong N, Song J et al. Polymer Thin Film Promotes Tumor Spheroid Formation via JAK2-STAT3 Signaling Primed by Fibronectin-Integrin a5 and Sustained by LMO2-LDB1 Complex Biomedicines 2022-10-24 [PMID: 36359204] (WB, Human)
• Koizumi M, Kama Y, Hirano KI et al. Transcription factor Zbtb1 interacts with bridging factor Lmo2 and maintains the T-lineage differentiation capacity of lymphoid progenitor cells Journal of Biological Chemistry 2022-11-01 [PMID: 36126774] (Western Blot, Immunohistochemistry-Paraffin, Human)
• Wang W, Meng Y, Chen Y et al. A comprehensive analysis of LMO2 pathogenic regulatory profile during T-lineage development and leukemic transformation Oncogene 2022-07-18 [PMID: 35851847] (Chemotaxis, Mouse)
• Hirano KI, Hosokawa H, Koizumi M Et al. LMO2 is essential to maintain the ability of progenitors to differentiate into T-cell lineage in mice eLife 2021-08-12 [PMID: 34382935] (Chemotaxis, WB, Mouse)
• Natkunam, Y et al. The oncoprotein LMO2 is expressed in normal germinal-center B cells and in human B-cell lymphomas. Blood;109(4):1636-42. 2007-02-15 [PMID: 17038524] (IHC-P, WB, Human)

Bioinformatics

• Gene Symbol: LMO2