PU.1/Spi-1 Antibody

Images

 
Western Blot: PU.1/Spi-1 Antibody [NBP2-27163] - PU.1/Spi1 Antibody [NBP2-27163] - Analysis of PU.1/Spi1 in U937 cell lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27163.
Flow Cytometry: PU.1/Spi-1 Antibody [NBP2-27163] - PU.1/Spi1 Antibody [NBP2-27163] - Intracellular analysis of PU.1 in mouse RAW cells (top) and PMA treated (20 ng/ml, overnight) human ThP1 cells (bottom) using 1.25 ...read more
Western Blot: PU.1/Spi1 Antibody [NBP2-27163] - analysis of 293T cell lysate (30ug) using anti-PU.1/Spi1 antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Bv, Ca, Eq, PmSpecies Glossary
Applications WB, Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml
   

Biological Strategies

     

Order Details

PU.1/Spi-1 Antibody Summary

Immunogen
A portion of amino acid 20-70 of human PU.1 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPI1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 4-6ug/ml
  • Flow Cytometry 1.25 ug/10^6 cells
Reviewed Applications
Read 1 Review rated 4
using
NBP2-27163 in the following applications:

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human, mouse, chimpanzee, monkey, dog, panda, horse, cow, and wild pig, 92% homologous in rat, 89% homologous in opossum,77% homologous in zebra finch and clearnose skate, and 71% homologous in chicken and dwarf caiman.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for PU.1/Spi-1 Antibody

  • hematopoietic transcription factor PU.1
  • OF
  • PU.1
  • SFPI1
  • SFPI131 kDa transforming protein
  • SPI-1 proto-oncogene
  • SPI1
  • Spi-1
  • SPI-A
  • spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein
  • transcription factor PU.1

Background

Transcription factor PU.1 belongs to the ETS family and contains one ETS DNA-binding domain. It is a novel caspase-3 substrate and found to contribute partially chemotherapeutic agents-induced apoptosis in leukemic cellsThis protein is also reported to activate gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. It is found to be one of the key regulators of hematopoitic cell development and induces apoptosis in myeloma cells through direct transactivation of an apoptosis-related gene, tumor necrosis factor (TNF)-related apoptosis inducing ligand (TRAIL).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for PU.1/Spi-1 Antibody (NBP2-27163) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-27163:
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Images Ratings Applications Species Date Details
Western Blot PU.1/Spi-1 NBP2-27163
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reviewed by:
Anonymous
WB Human 10/16/2014
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Summary

ApplicationWestern Blot
Sample Tested293T lysate
SpeciesHuman

Blocking

Blocking Details5% BSA in PBS

Primary Anitbody

Dilution Ratio1:2000 diluted in PBS with triton X-100, blot was incubated at room temperature on a rocker

Secondary Antibody

Secondary Descriptionanti-rabbit IgG HRP conjugate (Jackson Labs)
Secondary Manufacturer Cat#111-005-045
Secondary Concentration1:20000

Details

Detection NotesProbed using Visualizer Western Blot Detection Kit (Millipore) and captured on X-ray films, 30 sec exposure

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PU.1/Spi-1 Antibody (NBP2-27163) (0)

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Secondary Antibodies

 

Isotype Controls

Additional PU.1/Spi-1 Products

Bioinformatics Tool for PU.1/Spi-1 Antibody (NBP2-27163)

Discover related pathways, diseases and genes to PU.1/Spi-1 Antibody (NBP2-27163). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PU.1/Spi-1 Antibody (NBP2-27163)

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Pathways for PU.1/Spi-1 Antibody (NBP2-27163)

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PTMs for PU.1/Spi-1 Antibody (NBP2-27163)

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Recent Reviews

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5
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4
1
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2
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1
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Anonymous
10/16/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol SPI1
Entrez
Uniprot