GATA-3 Antibody [Unconjugated]

Images

 
Western blot shows lysates of MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human ...read more
GATA‑3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) for 3 hours at room temperature. ...read more
GATA‑3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at ...read more
GATA‑3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at ...read more
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. Specific bands were detected for GATA‑3 full length (FL) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

GATA-3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771
Specificity
Detects human and mouse GATA-3 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GATA3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 2.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF2605 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-3 Antibody [Unconjugated]

  • 131320
  • GATA binding protein 3
  • GATA3
  • GATA-3
  • GATA-binding factor 3
  • GATA-binding protein 3
  • HDRMGC5199
  • MGC2346
  • MGC5445
  • trans-acting T-cell-specific transcription factor GATA-3

Background

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development and the maintenance of the differentiated state in epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GATA-3 Antibody (AF2605)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC, IHC, IHC Not Specified, IHC Paraffin-embedded, WB.


Filter By Application
ICC
(1)
IHC
(1)
IHC Not Specified
(1)
IHC Paraffin-embedded
(1)
WB
(3)
All Applications
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Human
(5)
Mouse
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF2605 Applications Species
P Shahi, CY Wang, J Chou, C Hagerling, H Gonzalez V, A Ruderisch, Y Yu, MD Lai, Z Werb GATA3 targets semaphorin 3B in mammary epithelial cells to suppress breast cancer progression and metastasis Oncogene, 2017;0(0):. 2017 [PMID: 28581515] (WB,, Human) WB Human
Takasato M, Er P, Chiu H, Maier B, Baillie G, Ferguson C, Parton R, Wolvetang E, Roost M, Chuva de Sousa Lopes S, Little M Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. Nature, 2015;526(7574):564-8. 2015 [PMID: 26444236] (IHC Not Specified,, Human) IHC Not Specified Human
Tabata T, Petitt M, Zydek M, Fang-Hoover J, Larocque N, Tsuge M, Gormley M, Kauvar L, Pereira L Human cytomegalovirus infection interferes with the maintenance and differentiation of trophoblast progenitor cells of the human placenta. J Virol, 2015;89(9):5134-47. 2015 [PMID: 25741001] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Yu, Wei-Ming, Appler, Jessica, Kim, Ye-Hyun, Nishitani, Allison, Holt, Jeffrey, Goodrich, Lisa V A Gata3-Mafb transcriptional network directs post-synaptic differentiation in synapses specialized for hearing. Elife, 2013;2(0):e01341. 2013 [PMID: 24327562] (IHC,, Mouse) IHC Mouse
Pellegrino MJ, Parrish DC, Zigmond RE, Habecker BA Cytokines inhibit norepinephrine transporter expression by decreasing Hand2. Mol. Cell. Neurosci., 2011;46(3):671-80. 2011 [PMID: 21241805] (WB,, Human) WB Human
Song H, Suehiro J, Kanki Y, Kawai Y, Inoue K, Daida H, Yano K, Ohhashi T, Oettgen P, Aird WC, Kodama T, Minami T Critical role for GATA3 in mediating Tie2 expression and function in large vessel endothelial cells. J. Biol. Chem., 2009;284(42):29109-24. 2009 [PMID: 19674970] (ICC,, Human) ICC Human
Ren Y, Lu L, Guo TB, Qiu J, Yang Y, Liu A, Zhang JZ Novel immunomodulatory properties of berbamine through selective down-regulation of STAT4 and action of IFN-gamma in experimental autoimmune encephalomyelitis. J. Immunol., 2008;181(2):1491-8. 2008 [PMID: 18606704] (WB,, Mouse) WB Mouse

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Bioinformatics

Gene Symbol GATA3
Entrez
Uniprot