GATA-3 Antibody [Unconjugated]

Images

 
Western blot shows lysates of MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human ...read more
GATA‑3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at ...read more
GATA‑3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) for 3 hours at room temperature. ...read more
GATA‑3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at ...read more
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. Specific bands were detected for GATA‑3 full length (FL) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

GATA-3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771
Specificity
Detects human and mouse GATA-3 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GATA3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 2.5 ug/mL
  • Western Blot 0.25 ug/mL
Publications
Read Publications using
AF2605 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-3 Antibody [Unconjugated]

  • 131320
  • GATA binding protein 3
  • GATA3
  • GATA-3
  • GATA-binding factor 3
  • GATA-binding protein 3
  • HDRMGC5199
  • MGC2346
  • MGC5445
  • trans-acting T-cell-specific transcription factor GATA-3

Background

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development and the maintenance of the differentiated state in epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GATA-3 Antibody (AF2605)(21)

We have publications tested in 4 confirmed species: Human, Mouse, Primate - Cholrocebus pygerythrus (Vervet Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 6 applications: ChIP, Flow Cytometry, ICC, IHC, IHC-P, Western Blot.


Filter By Application
ChIP
(1)
Flow Cytometry
(1)
ICC
(5)
IHC
(12)
IHC-P
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(15)
Mouse
(5)
Primate - Cholrocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using AF2605 Applications Species
Yu, L;Logsdon, D;Pinzon-Arteaga, CA;Duan, J;Ezashi, T;Wei, Y;Ribeiro Orsi, AE;Oura, S;Liu, L;Wang, L;Liu, K;Ding, X;Zhan, L;Zhang, J;Nahar, A;Stobbe, C;Katz-Jaffe, M;Schoolcraft, WB;Tan, T;Hon, GC;Yuan, Y;Wu, J; Large-scale production of human blastoids amenable to modeling blastocyst development and maternal-fetal cross talk Cell stem cell 2023-09-07 [PMID: 37683605] (ICC, Human) ICC Human
J Li, Q Zhu, J Cao, Y Liu, Y Lu, Y Sun, Q Li, Y Huang, S Shang, X Bian, C Li, L Zhang, Y Wang, Y Nie, J Fu, W Li, MA Mazid, Y Jiang, W Jia, X Wang, Y Sun, MA Esteban, Q Sun, F Zhou, Z Liu Cynomolgus monkey embryo model captures gastrulation and early pregnancy Cell Stem Cell, 2023-04-06;30(4):362-377.e7. 2023-04-06 [PMID: 37028403] (IHC, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)) IHC Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
K Hiratsuka, T Miyoshi, KT Kroll, NR Gupta, MT Valerius, T Ferrante, M Yamashita, JA Lewis, R Morizane Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery Science Advances, 2022-09-21;8(38):eabq0866. 2022-09-21 [PMID: 36129975] (IHC, Human) IHC Human
M Ohgushi, N Taniyama, A Vandenbon, M Eiraku Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model Cell Reports, 2022-06-21;39(12):110973. 2022-06-21 [PMID: 35732132] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
D Meisterman, A Bruneau, S Loubersac, A Reignier, J Firmin, V François-C, S Kilens, Y Lelièvre, J Lammers, M Feyeux, P Hulin, S Nedellec, B Bretin, G Castel, N Allègre, S Covin, A Bihouée, M Soumillon, T Mikkelsen, P Barrière, C Chazaud, J Chappell, V Pasque, J Bourdon, T Fréour, L David Integrated pseudotime analysis of human pre-implantation embryo single-cell transcriptomes reveals the dynamics of lineage specification Cell Stem Cell, 2021-05-17;0(0):. 2021-05-17 [PMID: 34004179] (IHC, Human) IHC Human
SE Howden, SB Wilson, E Groenewege, L Starks, TA Forbes, KS Tan, JM Vanslambro, EM Holloway, YH Chen, S Jain, JR Spence, MH Little Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk Cell Stem Cell, 2020-12-29;0(0):. 2020-12-29 [PMID: 33378647] (IHC, Human) IHC Human
K Uchimura, H Wu, Y Yoshimura, BD Humphreys Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling Cell Reports, 2020-12-15;33(11):108514. 2020-12-15 [PMID: 33326782] (IHC, Human) IHC Human
MN Shahbazi, T Wang, X Tao, BAT Weatherbee, L Sun, Y Zhan, L Keller, GD Smith, A Pellicer, RT Scott, E Seli, M Zernicka-G Developmental potential of aneuploid human embryos cultured beyond implantation Nat Commun, 2020-08-10;11(1):3987. 2020-08-10 [PMID: 32778678] (ICC, IHC, Human) ICC, IHC Human
AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis Cell Rep, 2020-04-07;31(1):107471. 2020-04-07 [PMID: 32268087] (ICC, Human) ICC Human
Z Tan, A Rak-Raszew, I Skovorodki, SJ Vainio Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis Cells, 2020-01-31;9(2):. 2020-01-31 [PMID: 32023845] (IHC, Mouse) IHC Mouse
Show All 21 Publications.

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Bioinformatics

Gene Symbol GATA3
Uniprot