GATA-3 Antibody [Unconjugated]


Western blot shows lysates of MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human more
GATA‑3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) for 3 hours at room temperature. more
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. Specific bands were detected for GATA‑3 full length (FL) at more
GATA‑3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at more
GATA‑3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF

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GATA-3 Antibody [Unconjugated] Summary

E. coli-derived recombinant human GATA-3
Accession # P23771
Detects human and mouse GATA-3 in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 2.5 ug/mL
  • Western Blot 0.25 ug/mL
Read Publications using
AF2605 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-3 Antibody [Unconjugated]

  • 131320
  • GATA binding protein 3
  • GATA3
  • GATA-3
  • GATA-binding factor 3
  • GATA-binding protein 3
  • HDRMGC5199
  • MGC2346
  • MGC5445
  • trans-acting T-cell-specific transcription factor GATA-3


GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development and the maintenance of the differentiated state in epithelial cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GATA-3 Antibody (AF2605)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ChIP, ICC, IHC, IHC-P, Western Blot.

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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF2605 Applications Species
MN Shahbazi, T Wang, X Tao, BAT Weatherbee, L Sun, Y Zhan, L Keller, GD Smith, A Pellicer, RT Scott, E Seli, M Zernicka-G Developmental potential of aneuploid human embryos cultured beyond implantation Nat Commun, 2020;11(1):3987. 2020 [PMID: 32778678] (ICC, Human) ICC Human
AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis Cell Rep, 2020;31(1):107471. 2020 [PMID: 32268087] (ICC, Human) ICC Human
S Nomura, H Takahashi, J Suzuki, M Kuwahara, M Yamashita, T Sawasaki Pyrrothiogatain acts as an inhibitor of GATA family proteins and inhibits Th2 cell differentiation in vitro Sci Rep, 2019;9(1):17335. 2019 [PMID: 31758034] (ChIP, Mouse) ChIP Mouse
F Schutgens, MB Rookmaaker, T Margaritis, A Rios, C Ammerlaan, J Jansen, L Gijzen, M Vormann, A Vonk, M Viveen, FY Yengej, S Derakhshan, KM de Winter-, B Artegiani, R van Boxtel, E Cuppen, APA Hendrickx, MM van den He, E Heitzer, H Lanz, J Beekman, JL Murk, R Masereeuw, F Holstege, J Drost, MC Verhaar, H Clevers Tubuloids derived from human adult kidney and urine for personalized disease modeling Nat. Biotechnol., 2019;37(3):303-313. 2019 [PMID: 30833775] (IHC, Human) IHC Human
B Phipson, PX Er, AN Combes, TA Forbes, SE Howden, L Zappia, HJ Yen, KT Lawlor, LJ Hale, J Sun, E Wolvetang, M Takasato, A Oshlack, MH Little Evaluation of variability in human kidney organoids Nat. Methods, 2019;16(1):79-87. 2019 [PMID: 30573816] (IHC, Mouse) IHC Mouse
MY Turco, L Gardner, RG Kay, RS Hamilton, M Prater, MS Hollinshea, A McWhinnie, L Esposito, R Fernando, H Skelton, F Reimann, FM Gribble, A Sharkey, SGE Marsh, S O'Rahilly, M Hemberger, GJ Burton, A Moffett Trophoblast organoids as a model for maternal-fetal interactions during human placentation Nature, 2018;0(0):. 2018 [PMID: 30487605] (IHC, Human) IHC Human
P Shahi, CY Wang, J Chou, C Hagerling, H Gonzalez V, A Ruderisch, Y Yu, MD Lai, Z Werb GATA3 targets semaphorin 3B in mammary epithelial cells to suppress breast cancer progression and metastasis Oncogene, 2017;0(0):. 2017 [PMID: 28581515] (Western Blot, Human) Western Blot Human
Takasato M, Er P, Chiu H, Maier B, Baillie G, Ferguson C, Parton R, Wolvetang E, Roost M, Chuva de Sousa Lopes S, Little M Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. Nature, 2015;526(7574):564-8. 2015 [PMID: 26444236] (IHC, Human) IHC Human
Tabata T, Petitt M, Zydek M, Fang-Hoover J, Larocque N, Tsuge M, Gormley M, Kauvar L, Pereira L Human cytomegalovirus infection interferes with the maintenance and differentiation of trophoblast progenitor cells of the human placenta. J Virol, 2015;89(9):5134-47. 2015 [PMID: 25741001] (IHC-P, Human) IHC-P Human
Yu , Wei-Ming, Appler , Jessica, Kim , Ye-Hyun, Nishitani , Allison, Holt , Jeffrey, Goodrich , Lisa V A Gata3-Mafb transcriptional network directs post-synaptic differentiation in synapses specialized for hearing. Elife, 2013;2(0):e01341. 2013 [PMID: 24327562] (IHC, Mouse) IHC Mouse
Show All 13 Publications.

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Secondary Antibodies


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Other Available Formats

Biotin BAF2605

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Gene Symbol GATA3