GATA-1 Antibody (4F5)

Western Blot: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis using GATA1 mouse mAb against K562 (1) cell lysate.

Immunocytochemistry/Immunofluorescence: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of K562(left) cells using GATA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry: GATA-1 Antibody (4F5) [NBP1-47492] - Staining of Fetal Baboon Liver. Antigen retrieval: 10mM sodium citrate, pH 6.0, Primary Ab: 1:500 dilution, Secondary Ab (biotinylated): 1:200, Streptavidin-AlexaFluor488: 1:500 (green), Quench autofluorescence with 1% Sudan Black in 70% methanol. Image from verified customer review.

Immunohistochemistry-Paraffin: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of pancreatic cancer, using GATA1 mouse mAb with DAB staining.

• Reactivity: Hu, Pm
• Applications: WB, ELISA, ICC/IF, IHC, ChIP
• Clone: 4F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GATA-1 Antibody (4F5) Summary

• Immunogen: Purified recombinant fragment of human GATA-1 expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GATA1
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: Use in Chip reported in scientific literature (PMID:32763160)
• Theoretical MW: 42.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Papio anubis reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Ascites

Alternate Names for GATA-1 Antibody (4F5)

• Eryf1
• ERYF1GATA-binding protein 1 (globin transcription factor 1)
• GATA binding protein 1 (globin transcription factor 1)
• GATA1
• GATA-1
• GATA-1erythroid transcription factor
• GATA-binding factor 1
• GF-1
• GF1globin transcription factor 1
• NF-E1 DNA-binding protein
• NFE1erythroid transcription factor 1
• transcription factor GATA1
• XLTT

Background

GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GATA-1 Antibody (NBP1-47492)(2)

Publications using NBP1-47492

• Chen J, Zhou Q, Liu MH et al. FAM122A Inhibits Erythroid Differentiation through GATA1 Stem Cell Reports 2020-07-31 [PMID: 32763160] (ICC/IF, Chemotaxis, Human)
• Astrom M, Hahn-Stromberg V, Zetterberg E et al. X-linked thrombocytopenia with thalassaemia displays bone marrow reticulin fibrosis and enhanced angiogenesis: Comparisons with primary myelofibrosis. Am. J. Hematol. 2014-11-25 [PMID: 25421114]

Review for GATA-1 Antibody (NBP1-47492) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Papio anubis.

reviewed by: Verified Customer IHC-P Papio anubis 02/26/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: fetal liver
• Species: Papio anubis
• Lot: 100706

Comments

• Comments: IHC paraffin, species Papio anubis (Olive Baboon), fetal liver
  Antigen retrieval 10mM sodium citrate, pH 6.0
  Image-iT signal enhancer
  Streptavidin/Biotin blocking kit
  Primary Ab: 1:500 dilution
  Second Ab (biotinylated): 1:200
  Streptavidin-AlexaFluor488: 1:500 (green)
  Quench autofluorescence with 1% Sudan Black in 70% methanol
  Counterstain nuclei with propidium iodide (red)

Bioinformatics

• Gene Symbol: GATA1