GATA-1 Antibody (4F5) - BSA Free

Images

 
Western Blot: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis using GATA1 mouse mAb against K562 (1) cell lysate.
Immunocytochemistry/ Immunofluorescence: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of K562(left) cells using GATA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: GATA-1 Antibody (4F5) [NBP1-47492] - Staining of Fetal Baboon Liver. Antigen retrieval: 10mM sodium citrate, pH 6.0, Primary Ab: 1:500 dilution, Secondary Ab (biotinylated): 1:200, ...read more
Immunohistochemistry-Paraffin: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of pancreatic cancer, using GATA1 mouse mAb with DAB staining.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, ChIP
Clone
4F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

GATA-1 Antibody (4F5) - BSA Free Summary

Immunogen
Purified recombinant fragment of human GATA-1 expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GATA1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
Use in Chip reported in scientific literature (PMID:32763160)
Theoretical MW
42.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-47492 in the following applications:

Publications
Read Publications using
NBP1-47492 in the following applications:

Reactivity Notes

Papio anubis reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for GATA-1 Antibody (4F5) - BSA Free

  • Eryf1
  • ERYF1GATA-binding protein 1 (globin transcription factor 1)
  • GATA binding protein 1 (globin transcription factor 1)
  • GATA1
  • GATA-1
  • GATA-1erythroid transcription factor
  • GATA-binding factor 1
  • GF-1
  • GF1globin transcription factor 1
  • NF-E1 DNA-binding protein
  • NFE1erythroid transcription factor 1
  • transcription factor GATA1
  • XLTT

Background

GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GATA-1 Antibody (NBP1-47492)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Chemotaxis, ICC/IF.


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Chemotaxis
(1)
ICC/IF
(1)
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Human
(1)
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Review for GATA-1 Antibody (NBP1-47492) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Papio anubis.

Reviews using NBP1-47492:
Filter by Applications
IHC-P
(1)
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Filter by Species
Papio anubis
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GATA-1 NBP1-47492
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4
reviewed by:
Verified Customer
IHC-P Papio anubis 02/26/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedfetal liver
SpeciesPapio anubis
Lot100706

Comments

CommentsIHC paraffin, species Papio anubis (Olive Baboon), fetal liver
Antigen retrieval 10mM sodium citrate, pH 6.0
Image-iT signal enhancer
Streptavidin/Biotin blocking kit
Primary Ab: 1:500 dilution
Second Ab (biotinylated): 1:200
Streptavidin-AlexaFluor488: 1:500 (green)
Quench autofluorescence with 1% Sudan Black in 70% methanol
Counterstain nuclei with propidium iodide (red)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GATA-1 Antibody (NBP1-47492) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for GATA-1 Antibody (NBP1-47492)

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Blogs on GATA-1

There are no specific blogs for GATA-1, but you can read our latest blog posts.
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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Verified Customer
02/26/2019
Application: IHC-P
Species: Papio anubis

Bioinformatics

Gene Symbol GATA1