Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free

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Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Paraffin-embedded human salivary gland tissue. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Simple Western, Flow, IHC-P, CyTOF-ready, ICC/IF
Clone
OV-TL12/30
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free Summary

Immunogen
OTN 11, ovarian carcinoma cell line
Localization
Cytoplasmic
Marker
Glandular and Transitional Epithelial Marker
Specificity
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT7
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 1.0 ug/ml
  • Flow Cytometry 5 - 10 ul/million cells in 0.1ml
  • Immunohistochemistry-Paraffin 1:100 - 1:200
  • CyTOF-ready
  • Immunofluorescence 1:50 - 1:100
Application Notes
Hu-chromosome location: 12q13.13 Immunohistochemistry-Paraffin 1:100 - 1:200 for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Cytokeratin 7 Lysate (NBP2-64969)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-47940 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free

  • CK7
  • CK-7
  • cytokeratin 7
  • cytokeratin-7
  • K2C7
  • K7keratin, 55K type II cytoskeletal
  • keratin 7
  • keratin, type II cytoskeletal 7
  • keratin-7
  • MGC129731
  • MGC3625
  • sarcolectin
  • SCLkeratin, simple epithelial type I, K7
  • type II mesothelial keratin K7
  • Type-II keratin Kb7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytokeratin 7 Antibody (NBP2-47940) (0)

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Review for Cytokeratin 7 Antibody (NBP2-47940) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-47940:
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 Cytokeratin 7 NBP2-47940
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reviewed by:
Anonymous
Human 09/23/2018
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Summary

Sample TestedSalivary gland tissue
SpeciesHuman
LotI don't have it any more

Comments

CommentspH9 antigen retrieval

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Anonymous
09/23/2018
Application:
Species: Human

Bioinformatics

Gene Symbol KRT7