Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free

Images

 
Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by ...read more
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)
Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 7 Antibody (OV-TL12/30) followed by Goat anti-Mouse IgG-CF488 (Blue); ...read more
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Paraffin-embedded human salivary gland tissue. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Lane view shows a specific band for Cytokeratin 7 at 5 ug/mL in HeLa cell lysate.Electropherogram image of corresponding Simple ...read more
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 7 antibody was used at 1 ug/mL dilution of HeLa ...read more
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Simple Western lane view shows a specific band for Cytokeratin 7 in 0.1 mg/mL of HeLa lysate(s). This experiment was performed ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, ICC/IF
Clone
OV-TL12/30
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free Summary

Immunogen
OTN 11, ovarian carcinoma cell line
Localization
Cytoplasmic
Marker
Glandular and Transitional Epithelial Marker
Specificity
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT7
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Simple Western 5 ug/ml
  • Flow Cytometry : 5 - 10 ul/million cells in 0.1ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin : 1:100-1:200
  • CyTOF-ready
  • Immunofluorescence : 1:50 - 1:100
Application Notes
Hu-chromosome location: 12q13.13 Immunohistochemistry-Paraffin 1:100 - 1:200 for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Cytokeratin 7 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP2-47940 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30522949).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free

  • CK7
  • CK-7
  • cytokeratin 7
  • cytokeratin-7
  • K2C7
  • K7keratin, 55K type II cytoskeletal
  • keratin 7
  • keratin, type II cytoskeletal 7
  • keratin-7
  • MGC129731
  • MGC3625
  • sarcolectin
  • SCLkeratin, simple epithelial type I, K7
  • type II mesothelial keratin K7
  • Type-II keratin Kb7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytokeratin 7 Antibody (NBP2-47940) (0)

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Review for Cytokeratin 7 Antibody (NBP2-47940) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-47940:
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Images Ratings Applications Species Date Details
 Cytokeratin 7 NBP2-47940
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reviewed by:
Anonymous
Human 09/23/2018
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Summary

Sample TestedSalivary gland tissue
SpeciesHuman
LotI don't have it any more

Comments

CommentspH9 antigen retrieval

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 7 Antibody (NBP2-47940) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47940AF350
Alexa Fluor 405 NBP2-47940AF405
Alexa Fluor 488 NBP2-47940AF488
Alexa Fluor 532 NBP2-47940AF532
Alexa Fluor 594 NBP2-47940AF594
Alexa Fluor 647 NBP2-47940AF647
Alexa Fluor 700 NBP2-47940AF700
Alexa Fluor 750 NBP2-47940AF750
Allophycocyanin NBP2-47940APC
Biotin NBP2-47940B
DyLight 350 NBP2-47940UV
DyLight 405 NBP2-47940V
DyLight 488 NBP2-47940G
DyLight 550 NBP2-47940R
DyLight 594 NBP2-47940DL594
DyLight 650 NBP2-47940C
DyLight 680 NBP2-47940FR
DyLight 755 NBP2-47940IR
FITC NBP2-47940F
HRP NBP2-47940H
Janelia Fluor 549 NBP2-47940JF549
Janelia Fluor 646 NBP2-47940JF646
PE NBP2-47940PE
PerCP NBP2-47940PCP

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Recent Reviews

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1
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Anonymous
09/23/2018
Application:
Species: Human

Bioinformatics

Gene Symbol KRT7