Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free

Images

 
Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by ...read more
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Formalin-fixed, paraffin-embeddedhuman prsotatecarcinoma stained with Biotin-conjugated Cytokeratin 7 Mouse ...read more
Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 7 Antibody (OV-TL12/30) followed by Goat anti-Mouse IgG-CF488 (Blue); ...read more
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Paraffin-embedded human salivary gland tissue. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Formalin-fixed, paraffin-embeddedhuman prostatecarcinoma stained with Biotin-conjugated Cytokeratin 7 Mouse ...read more
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Lane view shows a specific band for Cytokeratin 7 at 5 ug/mL in HeLa cell lysate.Electropherogram image of corresponding Simple ...read more
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 7 antibody was used at 1 ug/mL dilution of HeLa ...read more
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Simple Western lane view shows a specific band for Cytokeratin 7 in 0.1 mg/mL of HeLa lysate(s). This experiment was performed ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, ICC/IF
Clone
OV-TL12/30
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free Summary

Immunogen
OTN 11, ovarian carcinoma cell line
Localization
Cytoplasmic
Marker
Glandular and Transitional Epithelial Marker
Specificity
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT7
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry : 5 - 10 ul/million cells in 0.1ml
  • Immunocytochemistry/Immunofluorescence
  • Immunofluorescence : 1:50 - 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin : 1:100-1:200
  • Simple Western 5 ug/ml
  • Western Blot
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30239759). Use in Immunohistochemistry reported in scientific literature (PMID: 30522949).
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-47940 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30522949).

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Notes

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44814).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free

  • CK7
  • CK-7
  • cytokeratin 7
  • cytokeratin-7
  • K2C7
  • K7keratin, 55K type II cytoskeletal
  • keratin 7
  • keratin, type II cytoskeletal 7
  • keratin-7
  • MGC129731
  • MGC3625
  • sarcolectin
  • SCLkeratin, simple epithelial type I, K7
  • type II mesothelial keratin K7
  • Type-II keratin Kb7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytokeratin 7 Antibody (NBP2-47940) (0)

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Review for Cytokeratin 7 Antibody (NBP2-47940) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-47940:
Filter by Applications
Immunofluorescence - Paraffin
(1)
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Images Ratings Applications Species Date Details
Other Cytokeratin 7 NBP2-47940
Enlarge
5
reviewed by:
Christian Schuerch
Immunofluorescence - Paraffin Human 09/23/2018
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Summary

ApplicationOther
Sample TestedSalivary gland tissue
SpeciesHuman
LotI don't have it any more

Comments

CommentspH9 antigen retrieval

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Recent Reviews

5
5
1
4
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2
0
1
0

Christian Schuerch
09/23/2018
Application: Immunofluorescence - Paraffin
Species: Human

Bioinformatics

Gene Symbol KRT7