Gastric Lipase Antibody [DyLight 405]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 405

Gastric Lipase Antibody [DyLight 405] Summary

Immunogen
A synthetic peptide within residues 1-100 of human Gastric Lipase. [Swiss-Prot# P07098]
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIPF
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This Gastric Lipase antibody is useful for Western blot analysis, where a band is seen at ~45 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and mouse. Immunogen sequence has 87% homology to canine.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Gastric Lipase Antibody [DyLight 405]

  • EC 3.1.1
  • EC 3.1.1.3
  • Gastric lipase
  • gastric triacylglycerol lipase
  • GL
  • HGL
  • HLAL
  • lipase, gastric
  • MGC138477
  • MGC142271

Background

Gastric lipase (GL/LIPF or gastric triacylglycerol lipase) is an extremophilic enzyme which retains its activity in gastric juices fully at pH 2 under the presence of pepsin in stomach wherein the gastrointestinal lipolysis of dietary fat is initiated. Gastric lipase belongs to AB hydrolase superfamily/lipase family and is a secreted protein which is not inhibited by bile salts in the gastrointestinal tract, whereas pancreatic lipase (main lipase involved in fat digestion) requires a cofactor, namely colipase, to counteract the inhibitory effects of bile salts on lipase adsorption at lipid-water interfaces. Due to its acid resistant properties, it has been proposed as a candidate for the treatment of pancreatic enzyme insufficiency observed in chronic pancreatitis and cystic fibrosis, the gastrointestinal disorders which are characterized by exocrine pancreatic secretion deficiency, gastric acid secretion not being neutralized by pancreatic bicarbonate, and pH of the small intestine contents reaching as low as 2-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Gastric Lipase Antibody (NB110-60930V)

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Bioinformatics

Gene Symbol LIPF