Gastric Lipase Antibody [FITC]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: FITC

Gastric Lipase Antibody [FITC] Summary

• Immunogen: A synthetic peptide within residues 1-100 of human Gastric Lipase. [Swiss-Prot# P07098]
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LIPF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This Gastric Lipase antibody is useful for Western blot analysis, where a band is seen at ~45 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Gastric Lipase Antibody [FITC]

• EC 3.1.1
• EC 3.1.1.3
• Gastric Lipase
• gastric triacylglycerol lipase
• GL
• HGL
• HLAL
• lipase, gastric
• LIPF
• MGC138477
• MGC142271

Background

Gastric lipase (GL/LIPF or gastric triacylglycerol lipase) is an extremophilic enzyme which retains its activity in gastric juices fully at pH 2 under the presence of pepsin in stomach wherein the gastrointestinal lipolysis of dietary fat is initiated. Gastric lipase belongs to AB hydrolase superfamily/lipase family and is a secreted protein which is not inhibited by bile salts in the gastrointestinal tract, whereas pancreatic lipase (main lipase involved in fat digestion) requires a cofactor, namely colipase, to counteract the inhibitory effects of bile salts on lipase adsorption at lipid-water interfaces. Due to its acid resistant properties, it has been proposed as a candidate for the treatment of pancreatic enzyme insufficiency observed in chronic pancreatitis and cystic fibrosis, the gastrointestinal disorders which are characterized by exocrine pancreatic secretion deficiency, gastric acid secretion not being neutralized by pancreatic bicarbonate, and pH of the small intestine contents reaching as low as 2-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIPF