Galectin-4 Antibody (9Z2B5)

Western Blot: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Western blot analysis of extracts of various cell lines, using Galectin-4 Rabbit mAb (NBP3-16249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry/Immunofluorescence: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunofluorescence analysis of human colon carcinoma using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunofluorescence analysis of mouse large intestine using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunohistochemistry of paraffin-embedded human appendix using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunohistochemistry of paraffin-embedded mouse colon using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunofluorescence analysis of rat rectum using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunoprecipitation analysis of 300ug extracts of HT-29 cells using 3ug Galectin-4 antibody (NBP3-16249). Western blot was performed from the immunoprecipitate using Galectin-4 antibody (NBP3-16249) at a dilition of 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clone: 9Z2B5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Galectin-4 Antibody (9Z2B5) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Galectin-4 (P56470).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LGALS4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:500 - 1:1000
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.05% BSA, 50% glycerol, pH7.3
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Galectin-4 Antibody (9Z2B5)

• Antigen NY-CO-27
• GAL4
• gal-4
• galectin 4
• Galectin4
• Galectin-4
• L-36 lactose-binding protein
• L36LBP
• Lactose-binding lectin 4
• lectin, galactoside-binding, soluble, 4
• LGALS4

Background

Galectins are a family of soluble beta-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One member of this family, Galectin-4, also known as Gal-4, L36 or LGALS4 maps to human chromosome 19q13.13 and encodes a 36-37 kDa protein. The Galectin-4 protein is composed of 323 amino acids and contains two homologous carbohydrate recognition domains (CRD) and all amino acids typically conserved in the galectin family. Expression of Galectin-4 correlates with the malignant potential of human hepatocellular carcinoma (HCC) and is differentially regulated depending on cell-cell contact, serum growth factors, cell growth and cell differentiation status. Galectin-4 expression is detected in epithelial cells of the colon, rectum, intestine, and in HT29 and LS174T cell lines. Galectin-4 is underexpressed in colorectal cancer and is preferentially upregulated in cells prone to peritoneal dissemination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LGALS4