Human/Mouse/Rat Phospho-CREB (S133) DuoSet IC ELISA, 2 Plate


A lysate prepared from HeLa human cervical epithelial carcinoma cells treated with 200 nM PMA for 20 minutes was analyzed with this DuoSet IC ELISA. The Human/Mouse/Rat Phospho-CREB (S133) DuoSet IC Detection Antibody more
Lysates prepared from HeLa human cervical epithelial carcinoma cells either uninduced or induced with 200 nM PMA for the indicated times were quantified with this DuoSet IC ELISA. The same lysates were also more
HeLa human cervical epithelial carcinoma cells were untreated or treated with 200 nM PMA for 20 minutes, either with or without U0126 at the indicated concentrations. Cells were lysed and CREB phosphorylated at S133 was more
Lysates prepared from mouse NIH/3T3 cells either uninduced or induced with 10 ng/mL of human PDGF (Catalog # 120-HD) for 5 minutes (left panel), and rat PC12 cells either uninduced or induced with 100 ng/mL of more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications ELISA

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Human/Mouse/Rat Phospho-CREB (S133) DuoSet IC ELISA, 2 Plate Summary

p Ser133
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using DYC2510-2.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human/Mouse/Rat Phospho-CREB (S133) DuoSet IC ELISA, 2 Plate

  • active transcription factor CREB
  • cAMP responsive element binding protein 1
  • cAMP-response element-binding protein-1
  • cAMP-responsive element-binding protein 1
  • CREB
  • CREB1
  • CREB-1
  • cyclic AMP-responsive element-binding protein 1
  • MGC9284
  • transactivator protein


The cAMP Response Element-Binding Protein (CREB) belongs to the bZIP superfamily of transcription factors, containing a basic domain that mediates DNA binding, and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. When phosphorylated at S133, CREB also binds the coactivator CREB binding protein (CBP), which enhances transcription by acetylating histones to facilitate chromatin unraveling.

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Publications for CREB (DYC2510-2)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

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Showing Publications 1 - 9 of 9.
Publications using DYC2510-2 Applications Species
D Jang, E Eliseeva, J Klubo-Gwie, S Neumann, MC Gershengor TSH stimulation of human thyroglobulin and thyroid peroxidase gene transcription is partially dependent on internalization Cellular Signalling, 2021-12-09;90(0):110212. 2021-12-09 [PMID: 34896620] (Human) Human
M Sisakht, Z Khoshdel, A Mahmoodazd, SM Shafiee, MA Takhshid Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons Iranian journal of basic medical sciences, 2021-07-01;24(7):978-985. 2021-07-01 [PMID: 34712429] (Rat) Rat
X Wan, S Guan, Y Hou, Y Qin, H Zeng, L Yang, Y Qiao, S Liu, Q Li, T Jin, Y Qiu, M Liu FOSL2 promotes VEGF-independent angiogenesis by transcriptionnally activating Wnt5a in breast cancer-associated fibroblasts Theranostics, 2021-03-05;11(10):4975-4991. 2021-03-05 [PMID: 33754039] (Human) Human
MK Jolly, KE Ware, S Xu, S Gilja, S Shetler, Y Yang, X Wang, RG Austin, D Runyambo, AJ Hish, S Bartholf D, JT George, RT Kreulen, MK Boss, AL Lazarides, DL Kerr, DG Gerber, D Sivaraj, AJ Armstrong, MW Dewhirst, WC Eward, H Levine, JA Somarelli E-cadherin represses anchorage-independent growth in sarcomas through both signaling and mechanical mechanisms Mol. Cancer Res., 2019-03-12;0(0):. 2019-03-12 [PMID: 30862685] (Human) Human
T Ayabe, R Ohya, K Kondo, Y Ano Iso-?-acids, bitter components of beer, prevent obesity-induced cognitive decline Sci Rep, 2018-03-19;8(1):4760. 2018-03-19 [PMID: 29555941] (Mouse) Mouse
J Gautam, S Banskota, S Shah, JG Jee, E Kwon, Y Wang, DY Kim, HW Chang, JA Kim 4-Hydroxynonenal-induced GPR109A activation elicits bipolar responses, G?i-mediated anti-inflammatory effects, and G??-mediated cell death Br. J. Pharmacol., 2018-05-22;0(0):. 2018-05-22 [PMID: 29473951] (Human) Human
MB Samad, MNAB Mohsin, BA Razu, MT Hossain, S Mahzabeen, N Unnoor, IA Muna, F Akhter, AU Kabir, JMA Hannan [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic ?-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Lepr(db/db) type 2 diabetic mice BMC Complement Altern Med, 2017-08-09;17(1):395. 2017-08-09 [PMID: 28793909] (Mouse) Mouse
Alborzinia H, Can S, Holenya P, Scholl C, Lederer E, Kitanovic I, Wolfl S Real-time monitoring of Cisplatin-induced cell death. PLoS ONE, 2011-05-16;6(5):e19714. 2011-05-16 [PMID: 21603599] (Human) Human
Holenya P, Kitanovic I, Heigwer F, Wolfl S Microarray-based kinetic colorimetric detection for quantitative multiplex protein phosphorylation analysis. Proteomics, 2011-04-18;11(10):2129-33. 2011-04-18 [PMID: 21500346] (Human) Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CREB (DYC2510-2). (Showing 1 - 1 of 1 FAQs).

  1. May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
    • We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.

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Blogs on CREB.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Exploring the Many Roles of PGC-1 alpha
The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in...  Read full blog post.

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Gene Symbol CREB1