Human/Mouse/Rat Phospho-CREB (S133) DuoSet IC ELISA, 2 Plate

A lysate prepared from HeLa human cervical epithelial carcinoma cells treated with 200 nM PMA for 20 minutes was analyzed with this DuoSet IC ELISA. The Human/Mouse/Rat Phospho-CREB (S133) DuoSet IC Detection Antibody was either untreated (no peptide) or pre-incubated with a phosphopeptide containing the CREB S133 phosphorylation site, a phosphopeptide containing the GSK-3 beta S9 phosphorylation site, a phosphopeptide containing the c-Jun S63 phosphorylation site, or a phosphopeptide containing the Raf1 S338 phosphorylation site. Peptides were used at 40 ng/mL. Only the phosphopeptide containing the CREB S133 phosphorylation site blocks the signal, indicating that the DuoSet IC ELISA is specific for CREB phosphorylation at S133.

Lysates prepared from HeLa human cervical epithelial carcinoma cells either uninduced or induced with 200 nM PMA for the indicated times were quantified with this DuoSet IC ELISA. The same lysates were also immunoblotted (inset) with either Anti-Human Phospho-CREB (S133) (Catalog # AF2510) or Anti-Human CREB (Catalog # AF2989) Polyclonal Antibodies. The DuoSet IC ELISA results correlate well with the amounts of phosphorylated CREB detected by Western blot. The immunoblot with anti-CREB antibody indicates that total levels of human CREB remained constant during incubation with PMA.The quantification of phosphorylated CREB with this DuoSet IC ELISA was also determined using cells pretreated with two concentrations of the MEK inhibitor U0126, which indirectly inhibits phosphorylation of CREB at S133.

HeLa human cervical epithelial carcinoma cells were untreated or treated with 200 nM PMA for 20 minutes, either with or without U0126 at the indicated concentrations. Cells were lysed and CREB phosphorylated at S133 was quantified with this DuoSet IC ELISA. The same lysates were also immunoblotted (inset) with either anti-phospho-CREB (S133) or anti-CREB antibodies. The DuoSet IC ELISA results correlate well with the amounts of phosphorylated CREB detected by Western blot. The immunoblot with anti-CREB antibody indicates that total levels of human CREB remained constant during the various treatments.The Phospho-CREB (S133) DuoSet IC ELISA also quantifies phosphorylated CREB levels in mouse and rat cell lysates.

Lysates prepared from mouse NIH/3T3 cells either uninduced or induced with 10 ng/mL of human PDGF (Catalog # 120-HD) for 5 minutes (left panel), and rat PC12 cells either uninduced or induced with 100 ng/mL of recombinant rat beta-NGF (Catalog # 556-NG) for 10 minutes (right panel), were quantified with this DuoSet IC ELISA. The same lysates were also immunoblotted (inset) with anti-phospho-CREB (S133) antibody. The DuoSet IC ELISA results correlate well with the amounts of phosphorylated CREB detected by Western blot.

• Reactivity: Hu, Mu, Rt
• Applications: ELISA
• Conjugate: Biotin

Human/Mouse/Rat Phospho-CREB (S133) DuoSet IC ELISA, 2 Plate Summary

• Modification: p Ser133
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CREB1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human/Mouse/Rat Phospho-CREB (S133) DuoSet IC ELISA, 2 Plate

• active transcription factor CREB
• cAMP responsive element binding protein 1
• cAMP-response element-binding protein-1
• cAMP-responsive element-binding protein 1
• CREB
• CREB1
• CREB-1
• cyclic AMP-responsive element-binding protein 1
• MGC9284
• transactivator protein

Background

The cAMP Response Element-Binding Protein (CREB) belongs to the bZIP superfamily of transcription factors, containing a basic domain that mediates DNA binding, and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. When phosphorylated at S133, CREB also binds the coactivator CREB binding protein (CBP), which enhances transcription by acetylating histones to facilitate chromatin unraveling.

Publications for CREB (DYC2510-2)(11)

Publications using DYC2510-2

• Akanchise, T;Luo, F;Angelov, B;Deng, Y;Manna, G;Angelova, A; Rapid structural transformation of ionizable lipid nanoparticles involving Omega-3 polyunsaturated fatty acids enhances antioxidant defense and mitochondrial proteins activity in pH-responsive drug delivery Journal of colloid and interface science 2026-02-15 [PMID: 41223481] (Human)
• Zhong, S;Wang, R;Gao, X;Guo, Q;Lin, R;Luo, M; Modular DNA barcoding of nanobodies enables multiplexed in situ protein imaging and high-throughput biomolecule detection eLife 2025-07-22 [PMID: 40694500] (Human)
• D Jang, E Eliseeva, J Klubo-Gwie, S Neumann, MC Gershengor TSH stimulation of human thyroglobulin and thyroid peroxidase gene transcription is partially dependent on internalization Cellular Signalling, 2021-12-09;90(0):110212. 2021-12-09 [PMID: 34896620] (Human)
• M Sisakht, Z Khoshdel, A Mahmoodazd, SM Shafiee, MA Takhshid Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons Iranian journal of basic medical sciences, 2021-07-01;24(7):978-985. 2021-07-01 [PMID: 34712429] (Rat)
• X Wan, S Guan, Y Hou, Y Qin, H Zeng, L Yang, Y Qiao, S Liu, Q Li, T Jin, Y Qiu, M Liu FOSL2 promotes VEGF-independent angiogenesis by transcriptionnally activating Wnt5a in breast cancer-associated fibroblasts Theranostics, 2021-03-05;11(10):4975-4991. 2021-03-05 [PMID: 33754039] (Human)
• MK Jolly, KE Ware, S Xu, S Gilja, S Shetler, Y Yang, X Wang, RG Austin, D Runyambo, AJ Hish, S Bartholf D, JT George, RT Kreulen, MK Boss, AL Lazarides, DL Kerr, DG Gerber, D Sivaraj, AJ Armstrong, MW Dewhirst, WC Eward, H Levine, JA Somarelli E-cadherin represses anchorage-independent growth in sarcomas through both signaling and mechanical mechanisms Mol. Cancer Res., 2019-03-12;0(0):. 2019-03-12 [PMID: 30862685] (Human)
• T Ayabe, R Ohya, K Kondo, Y Ano Iso-?-acids, bitter components of beer, prevent obesity-induced cognitive decline Sci Rep, 2018-03-19;8(1):4760. 2018-03-19 [PMID: 29555941] (Mouse)
• J Gautam, S Banskota, S Shah, JG Jee, E Kwon, Y Wang, DY Kim, HW Chang, JA Kim 4-Hydroxynonenal-induced GPR109A activation elicits bipolar responses, G?i-mediated anti-inflammatory effects, and G??-mediated cell death Br. J. Pharmacol., 2018-05-22;0(0):. 2018-05-22 [PMID: 29473951] (Human)
• MB Samad, MNAB Mohsin, BA Razu, MT Hossain, S Mahzabeen, N Unnoor, IA Muna, F Akhter, AU Kabir, JMA Hannan [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic ?-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Lepr(db/db) type 2 diabetic mice BMC Complement Altern Med, 2017-08-09;17(1):395. 2017-08-09 [PMID: 28793909] (Mouse)
• Alborzinia H, Can S, Holenya P, Scholl C, Lederer E, Kitanovic I, Wolfl S Real-time monitoring of Cisplatin-induced cell death. PLoS ONE, 2011-05-16;6(5):e19714. 2011-05-16 [PMID: 21603599] (Human)
• Holenya P, Kitanovic I, Heigwer F, Wolfl S Microarray-based kinetic colorimetric detection for quantitative multiplex protein phosphorylation analysis. Proteomics, 2011-04-18;11(10):2129-33. 2011-04-18 [PMID: 21500346] (Human)

FAQs for CREB (DYC2510-2). (Showing 1 - 2 of 2 FAQs).

1. May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
   • We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CREB1