A lysate prepared from HeLa human cervical epithelial carcinoma cells treated with 200 nM PMA for 20 minutes was analyzed with this DuoSet IC ELISA. The Human/Mouse/Rat Phospho-CREB (S133) DuoSet IC Detection Antibody ...read more
Lysates prepared from HeLa human cervical epithelial carcinoma cells either uninduced or induced with 200 nM PMA for the indicated times were quantified with this DuoSet IC ELISA. The same lysates were also ...read more
HeLa human cervical epithelial carcinoma cells were untreated or treated with 200 nM PMA for 20 minutes, either with or without U0126 at the indicated concentrations. Cells were lysed and CREB phosphorylated at S133 was ...read more
Lysates prepared from mouse NIH/3T3 cells either uninduced or induced with 10 ng/mL of human PDGF (Catalog # 120-HD) for 5 minutes (left panel), and rat PC12 cells either uninduced or induced with 100 ng/mL of ...read more
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Human/Mouse/Rat Phospho-CREB (S133) DuoSet IC ELISA, 2 Plate
active transcription factor CREB
cAMP responsive element binding protein 1
cAMP-response element-binding protein-1
cAMP-responsive element-binding protein 1
cyclic AMP-responsive element-binding protein 1
The cAMP Response Element-Binding Protein (CREB) belongs to the bZIP superfamily of transcription factors, containing a basic domain that mediates DNA binding, and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. When phosphorylated at S133, CREB also binds the coactivator CREB binding protein (CBP), which enhances transcription by acetylating histones to facilitate chromatin unraveling.
FAQs for CREB (DYC2510-2). (Showing 1 - 1 of 1 FAQs).
May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.
Exploring the Many Roles of PGC-1 alpha The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in... Read full blog post.