FXR/NR1H4 Antibody

Product Discontinued

FXR/NR1H4 Antibody Summary

• Immunogen: NR1H4 (NP_005114, 363 a.a. ~ 473 a.a) partial recombinant protein with GST tag.TPMFSFYKSIGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCKIHQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ
• Specificity: NR1H4 - nuclear receptor subfamily 1, group H, member 4
• Clonality: Polyclonal
• Host: Mouse
• Gene: NR1H4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: The quality control of this antibody is limited to Western blot on the immunizing protein. It has also been used for ELISA. Abnova's recommended working dilutions for western analysis are as follows: 1:500 dilution for ascites 1:1000 for purified Ig 1:500

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Unpurified antisera so the specific antibody concentration is unknown. Contains 50% glycerol.
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for FXR/NR1H4 Antibody

• BAR
• bile acid receptor
• farnesoid X receptor
• Farnesoid X-activated receptor
• Farnesol receptor HRR-1
• FXR
• FXRBAR
• HRR-1
• HRR1MGC163445
• NR1H4
• Nuclear receptor subfamily 1 group H member 4
• nuclear receptor subfamily 1, group H, member 4
• Retinoid X receptor-interacting protein 14
• RIP14
• RIP14RXR-interacting protein 14

Background

FXR and LXR fall into a category of proteins termed orphan receptors; because of their lack of a defined function, and in the case of LXR, the lack of a defined ligand. FXR has been shown to bind a class of lipid molecules called farnesoids. LXR/RXR heterodimers have highest affinity for DR-4 DNA elements while FXR/RXR heterodimers bind IR-1 elements. Both LXR/RXR and FXR/RXR heterodimers retain their responsiveness to 9-cis retinoic acid. The steroid receptor superfamily acts through direct association with DNA sequences known as hormone response elements (HREs) and bind DNA as either homo- or heterodimers. The promiscuous mediator of heterodimerization, RXR, is the receptor for 9-cis retinoic acid, and dimerizes with VDR, TR, PPAR, as well as several novel receptors including LXR (also referred to as RLD-1) and FXR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR1H4
• Entrez:
  • 9971(Human)
• OMIM:
  • 603826(Human)
• Uniprot:
  • NP_005114(Human)