CYP7A1 Antibody (946902) [Unconjugated]

CYP7A1 was detected in immersion fixed human hepatocytes induced with dexamethasone for 48 hours using Mouse Anti-Human CYP7A1 Polyclonal Antibody (Catalog # MAB9120) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (green). Specific staining was localized to endoplasmic reticula and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 946902
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CYP7A1 Antibody (946902) [Unconjugated] Summary

• Immunogen: Human CYP7A1 synthetic peptide
  Accession # P22680
• Specificity: Detects human CYP7A1 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYP7A1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CYP7A1 Antibody (946902) [Unconjugated]

• Cholesterol 7-alpha-hydroxylase
• cholesterol 7-alpha-monooxygenase
• CP7A
• CYP7
• CYP7A1
• CYP7MGC126826
• CYPVII
• Cytochrome P450 7A1
• cytochrome P450, family 7, subfamily A, polypeptide 1
• cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase)
• cytochrome P450, subfamily VIIA polypeptide 1
• EC 1.14.13
• EC 1.14.13.17
• MGC138389
• polypeptide 1

Background

CYP7A1 (cytochrome P450 7A1; also Cholesterol 7-alph-monooxygenase) is a 504 aa oxidoreductase enzyme (SwissProt #: P22680).  Human CYP7A1 shares 81% and 82% aa identity with mouse and rat CYP7A1, respectively.  It is expressed in the liver where it catalyzes cholesterol metabolism, which is the rate-limiting step in bile acid synthesis.  Disruption of CYP7A1 is associated with high plasma cholesterol levels leading to increased risk of coronary artery disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP7A1
• Uniprot:
  • P22680()