FoxP3 Cell Lysate

Western Blot: FOXP3 Cell Lysate [NBP2-25061] - 15 ul of human FOXP3 A) transfected cell lysate and B) mock transfected cell lysate probed with 5 ug/ml of Human FOXP3

Product Discontinued

FoxP3 Cell Lysate Summary

• Immunogen: FOXP3 cell lysates (transfected & mock transfected)
• Gene: FOXP3

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: HEK293 cells were transfected with full-length human FOXP3. Load 15 ul/lane of a mini-gel. This transfected cell lysate can be used as a positive western blot control for FOXP3

Packaging, Storage & Formulations

• Storage: Store at -80C in the dark. Avoid freeze-thaw cycles.
• Buffer: 1 x SDS sample buffer: 60 mM Tris-Hcl (pH 6.8) 10 mM EDTA 2 % SDS 0.03% Bromophenol Blue 10% glycerol 0.3% Beta-mercaptoethanol

Lysate Details for Array

• Type: Cell

Alternate Names for FoxP3 Cell Lysate

• AIID
• AIIDMGC141961
• DIETER
• forkhead box P3
• Forkhead Box Protein P3
• FoxP3
• FOXP3delta7
• immune dysregulation, polyendocrinopathy, enteropathy, X-linked
• Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
• IPEX
• JM2
• MGC141961
• MGC141963
• PIDX
• PIDXMGC141963
• SCURFIN
• XPID
• XPIDpolyendocrinopathy, enteropathy, X-linked

Background

Forkhead box P3 (FoxP3), aka Scurfin and JM2, is a protein involving in immune system responses and functioning as a transcriptional regulator crucial in the development and inhibitory of regulatory T-Cells (Treg). FoxP3 is a master regulator of the Treg lineage, who requires Ahr and STAT-5 and other additional transcription factors to retains its complete function and differentiation. Scurfin remains steady and constitutively expressed in two conditions: a high level in CD25 + CD4 positive regulatory T cells and a low level in CD4 positive/CD25 negative cells. JM2 is absent in CD4 negative/CD8 positive T cells. FoxP3 defects are related to various cancers and autoimmune diseases, and X-linked syndrome (IPEX), while overexpression may also cause severe immunodeficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for FoxP3 Lysate (NBP2-25061)(1)

Publication using NBP2-25061

• Lingblom C. Functional and Phenotypic Studies of Eosinophilic Granulocytes in Patients with Eosinophilic Esophagitis. Thesis. 2015-01-01 (WB)

FAQs for FoxP3 Lysate (NBP2-25061). (Showing 1 - 2 of 2 FAQ).

1. Does FoxP3 antibodies comes in lyophilized form?
   • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
2. What the theoretical molecular weight for FoxP3 antibodies?
   • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

Bioinformatics

• Gene Symbol: FOXP3