Home » FoxP3 » FoxP3 Kits » FoxP3 Flow Cytometry Kit

FoxP3 Flow Cytometry Kit

 Submit a Review

Images

 

Product Details

Summary
Product Discontinued
View other related FoxP3 Kits

Order Details


    • Catalog Number
      NBP2-29493
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

FoxP3 Flow Cytometry Kit Summary

Kit Type
Flow Cytometry Kit
Gene
FOXP3

Applications/Dilutions

Dilutions
  • Flow Cytometry 1ul/1 million cells

Packaging, Storage & Formulations

Storage
Storage is content dependent.
Buffer
1 x PBS
Preservative
0.05% Sodium Azide

Kit Components

Components
  1. Fixation/Permeabilization Concentrate 4X 15 ml
  2. Fixation/Permeabilization Diluent 1X 50 ml
  3. FOXP3 APC (Clone 3G3) 0.25 ml
  4. mCD25 PE 0.5 ml
  5. mCD4 FITC 0.5 ml
  6. mouse Fc block 50 ul
  7. mouse IgG1 APC Isotype 0.25 ml
  8. Permeabilization buffer 10X 50 ml
  9. Staining buffer 1X 3 X 60 ml

Alternate Names for FoxP3 Flow Cytometry Kit

  • AIID
  • AIIDMGC141961
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

Forkhead box P3 (FoxP3), aka Scurfin and JM2, is a protein involving in immune system responses and functioning as a transcriptional regulator crucial in the development and inhibitory of regulatory T-Cells (Treg). FoxP3 is a master regulator of the Treg lineage, who requires Ahr and STAT-5 and other additional transcription factors to retains its complete function and differentiation. Scurfin remains steady and constitutively expressed in two conditions: a high level in CD25 + CD4 positive regulatory T cells and a low level in CD4 positive/CD25 negative cells. JM2 is absent in CD4 negative/CD8 positive T cells. FoxP3 defects are related to various cancers and autoimmune diseases, and X-linked syndrome (IPEX), while overexpression may also cause severe immunodeficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(52 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
CD25/IL-2R alpha
[HRP]
DR2A00
N/A CD25/IL-2R alpha [HRP]
N/A CD25/IL-2R alpha [HRP]
(36 Publications)
Species: Hu
Applications: ELISA
ISG20 Antibody
(1B2-3C9) - Azide and ...
H00003669-M01
Western Blot ISG20 Antibody (1B2-3C9) - Azide and BSA Free
Western Blot ISG20 Antibody (1B2-3C9) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence ISG20 Antibody (1B2-3C9) - Azide and BSA Free
(1 Review)
(2 Publications)
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(307 Publications)
Species: Mu
Applications: ELISA
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(382 Publications)
Species: Hu
Applications: BA
CD8 Antibody
(53-6.7) - BSA Free
NBP1-49045
Immunohistochemistry-Paraffin CD8 Antibody (53-6.7) - BSA Free
Immunocytochemistry/ Immunofluorescence CD8 Antibody (53-6.7) - BSA Free
Immunohistochemistry-Frozen CD8 Antibody (53-6.7) - BSA Free
(3 Reviews)
(28 Publications)
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
IL-17/IL-17A
[Biotin]
DY421
N/A IL-17/IL-17A [Biotin]
(193 Publications)
Species: Mu
Applications: ELISA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(679 Publications)
Species: Mu
Applications: ELISA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(463 Publications)
Species: Hu
Applications: BA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(828 Publications)
Species: Hu
Applications: BA
CD3 Antibody (SP7)
NB600-1441
Immunohistochemistry-Frozen CD3 Antibody (SP7)
Immunohistochemistry-Paraffin CD3 Antibody (SP7)
Immunohistochemistry-Paraffin CD3 Antibody (SP7)
(3 Reviews)
(49 Publications)
Species: Ca, Hu, Mu, Po
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KD
GITR/TNFRSF18
Antibody (110416) [Un ...
MAB689
Flow Cytometry GITR/TNFRSF18 Antibody (110416) [Unconjugated]
(11 Publications)
Species: Hu
Applications: Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, WB
CD28 Antibody
(37407) [Unconjugated
MAB342
Agonist Activity CD28 Antibody (37407) [Unconjugated]
Immunocytochemistry CD28 Antibody (37407) [Unconjugated]
(1 Review)
(49 Publications)
Species: Hu
Applications: AgAct, ICC, WB
T-bet/TBX21
Antibody (39D) - BSA
NBP1-43299
Western Blot T-bet/TBX21 Antibody (39D) - BSA Free
(1 Review)
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
IL-7R alpha/CD127
Antibody (R34-34) - A ...
DDX0700P-100
Immunoprecipitation IL-7R alpha/CD127 Antibody (R34-34) - Azide and BSA Free
Flow Cytometry IL-7R alpha/CD127 Antibody (R34-34) - Azide and BSA Free
Flow Cytometry IL-7R alpha/CD127 Antibody (R34-34) - Azide and BSA Free
(40 Publications)
Species: Ca, Hu
Applications: B/N, Flow, IP
Doublecortin
Antibody
NBP1-72042
Western Blot Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
(5 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
Supplier Logo

Publications for FoxP3 Kit (NBP2-29493) (0)

There are no publications for FoxP3 Kit (NBP2-29493).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FoxP3 Kit (NBP2-29493) (0)

There are no reviews for FoxP3 Kit (NBP2-29493). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for FoxP3 Kit (NBP2-29493): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FoxP3 Kit (NBP2-29493). (Showing 1 - 2 of 2 FAQ).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

Additional FoxP3 Products

Research Areas for FoxP3 Kit (NBP2-29493)

Find related products by research area.

Blogs on FoxP3.

Check out the latest blog posts on FoxP3.
Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.
FOXP3
Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively, ...  Read full blog post.
FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of ...  Read full blog post.
FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom...  Read full blog post.

Contact Information

Product PDFs

  SDS
  NBP2-29493 Protocol
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our FoxP3 Flow Cytometry Kit and receive a gift card or discount.


Bioinformatics

Gene Symbol FOXP3