FoxP3 Antibody

Western Blot: FoxP3 Antibody [NB600-246] - FOXP3 Antibody [NB600-246] - Detection of murine FOXP3 using NB600-246. (1) Human CD4+CD25+ PBL, (2) HEK293T transfected with human Foxp3 cDNA, (3) 293/mouse foxp3, and (4) 293/empty vector

Product Discontinued

FoxP3 Antibody Summary

• Immunogen: A synthetic peptide made to the N-terminal region of human FOXP3 (between residues 1-50). [UniProt# Q9BZS1]
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FOXP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:150
  • Immunohistochemistry-Paraffin reported in scientific literature
  • Western Blot 1:1000-1:5000
• Application Notes: In Western blot, a band can be seen at ~47.2 kDa representing FOXP3. This antibody only detects transfected samples. It has not been shown to work on endogenous samples via Western blot.
• Theoretical MW: 47.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  FoxP3 Overexpression Lysate

• Control Peptide:

  FoxP3 Peptide (NB600-246PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FoxP3 Antibody

• AIID
• AIIDMGC141961
• DIETER
• forkhead box P3
• Forkhead Box Protein P3
• FoxP3
• FOXP3delta7
• immune dysregulation, polyendocrinopathy, enteropathy, X-linked
• Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
• IPEX
• JM2
• MGC141961
• MGC141963
• PIDX
• PIDXMGC141963
• SCURFIN
• XPID
• XPIDpolyendocrinopathy, enteropathy, X-linked

Background

FOXP3 (forkhead box protein P3) belongs to forkhead/winged-helix gene family of transcription factors that regulates the development and function of immune system. Localized in the nucleus, FOXP3 interacts with IKZF3 and is a crucial factor for the generation of CD4+CD25+ regulatory T cells (Tregs) and high levels expression of FOXP3 is essential for Treg's suppressive functions. SIRT1 facilitates FOXP3' decetylation, whereas, its acetylation on lysine residues causes its stabilization and promotes differentiation of T-cells into induced Tregs. Mutations of the FOXP3 gene lead to X-linked autoimmunity-allergic dysregulation syndrome in humans (also called immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome or IPEX) and the lymphoproliferative disease in Scurfy mouse. The FOXP3 X-linked recessive mutation results in lethality in hemizygous male mice soon after birth, and is characterized by excessive proliferation of CD4+ T cells, extensive multi-organ infiltration by leukocytes and systemic elevation of numerous cytokines. FoxP3 has also been shown to function as a tumor suppressor in several types of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FoxP3 Antibody (NB600-246)(3)

Publications using NB600-246

• Hagone S, Bharat P, Gupta A, Nitin G Expression of FOXP3 of Tumor-Infiltrating Lymphocytes in Invasive Breast Cancer: Its Relationship to Histopathological Parameters and Overall Survival I.P. Pavlov Russian Medical Biological Herald 2023-07-16 (IHC-P)
• Wilcox RA, Feldman AL, Wada DA et al. B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood :blood-2009-2004-216671. 2009-01-01 [PMID: 19597183] (IHC-P, Human)
• Lim, HW et al. Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells. J Immunol;175(7):4180-3. 2005-10-01 [PMID: 16177055]

FAQs for FoxP3 Antibody (NB600-246). (Showing 1 - 3 of 3 FAQs).

1. Does FoxP3 antibodies comes in lyophilized form?
   • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
2. What the theoretical molecular weight for FoxP3 antibodies?
   • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
3. What is the immunogen sequence of this FoxP3 antibody?
   • A synthetic sequence of amino acids 1 - 50 within the N-terminal region of Human FoxP3.

Bioinformatics

• Gene Symbol: FOXP3