FKBP52/FKBP4 Antibody (Hi52C) - BSA Free

Western Blot: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - Western Blot analysis of Human Cell lysates showing detection of FKBP52/FKBP4 protein using Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody, Clone Hi52C (NB110-96874). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody (NB110-96874) at 1.5 ug/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody, Clone Hi52C (NB110-96874). Tissue: MCF-7 cells (metastatic mammary gland/breast cell line). Species: Human. Primary Antibody: Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody (NB110-96874) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Courtesy of: Tom Ratajczak, Univ. of W. Australia.

Immunohistochemistry: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - Immunohistochemistry analysis using Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody, Clone Hi52C (NB110-96874). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody (NB110-96874) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Western Blot: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1.5 ug/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

• Reactivity: Hu, Mu, Rt, Ca, Ha
• Applications: WB, ICC/IF, IHC, IP
• Clone: Hi52C
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

FKBP52/FKBP4 Antibody (Hi52C) - BSA Free Summary

• Description: Novus Biologicals Mouse FKBP52/FKBP4 Antibody (Hi52C) - BSA Free (NB110-96874) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-FKBP52/FKBP4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to the residues of human FKBP52
• Localization: Cytoplasm , Nucleus
• Specificity: Detects approx 52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FKBP4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 5ug
  • Western Blot 1:2000
• Application Notes: 0.5 ug/ml was sufficient for detection of FKBP52 in 20 ug total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.FKBP52 Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for FKBP52/FKBP4 Antibody (Hi52C) - BSA Free

• 51 kDa FK506-binding protein
• 52 kDa FKBP
• 59 kDa immunophilin
• EC 5.2.1.8
• FK506 binding protein 4, 59kDa
• FK506-binding protein 4 (59kD)
• FK506-binding protein 4
• FKBP4
• FKBP-4
• FKBP51
• FKBP52
• FKBP-52
• FKBP52T-cell FK506-binding protein, 59kD
• FKBP59
• FKBP5952 kDa FK506-binding protein
• HBI
• HSP binding immunophilin
• Hsp56
• HSP-binding immunophilin
• Immunophilin FKBP52
• p52
• p59
• peptidyl-prolyl cis-trans isomerase FKBP4
• peptidylprolyl cis-trans isomerase
• PPIase FKBP4
• PPIase
• rotamase

Background

Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin Abinding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90 (4). Although FKBP52 and FKBP51 share approx. 75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90- binding characteristics (3, 5). Also, whereas FKBP51 typically has a role with the progesterone receptor, FKBP52 has been found to be linked to the progesterone, androgen and glucocorticoid receptors (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FKBP52/FKBP4 Antibody (NB110-96874)(1)

Publication using NB110-96874

• Kim MJ, Choi GE, Chae CW et al. Melatonin-mediated FKBP4 downregulation protects against stress-induced neuronal mitochondria dysfunctions by blocking nuclear translocation of GR Cell death & disease 2023-02-21 [PMID: 36810730] (IHC, WB, PLA, Human)

Bioinformatics

• Gene Symbol: FKBP4
• Entrez:
  • 2288(Human)
  • 14228(Mouse)
  • 260321(Rat)
• Uniprot:
  • Q02790()
  • NP_002005.1(Human)