FKBP52/FKBP4 Antibody (Hi52C)

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Western Blot: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1.5 ug/mL for 2 hours at RT. Secondary ...read more
Immunocytochemistry/ Immunofluorescence: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - Tissue: MCF-7 cells (metastatic mammary gland/breast cell line). Species: Human. Primary Antibody: Mouse Anti-FKBP52 Monoclonal ...read more
Immunohistochemistry-Paraffin: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Tissue: prostate tissue (ductual epithelial cells). Species: Human. Primary ...read more
Western Blot: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of FKBP52 protein using Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
Hi52C
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

FKBP52/FKBP4 Antibody (Hi52C) Summary

Immunogen
This protein representing FKBP52 in cell lysate. Also detects FKBP52 in whole tissue extracts from rat kidney and rat and mouse testes. Heavy chain migrates close to FKBP52 on SDS PAGE.Synthetic peptide corresponding to the residues of human FKBP52
Localization
Cytoplasm, Nucleus
Specificity
Detects approx 52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
FKBP4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 5 ug
Application Notes
0.5 ug/ml was sufficient for detection of FKBP52 in 20 ug total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.FKBP52 Antibody.

Reactivity Notes

Based on homology, It is predicted to detect Mouse, Rat, Canine and Hamster

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for FKBP52/FKBP4 Antibody (Hi52C)

  • 51 kDa FK506-binding protein
  • 52 kDa FKBP
  • 59 kDa immunophilin
  • EC 5.2.1.8
  • FK506 binding protein 4, 59kDa
  • FK506-binding protein 4 (59kD)
  • FK506-binding protein 4
  • FKBP4
  • FKBP-4
  • FKBP51
  • FKBP52
  • FKBP-52
  • FKBP52T-cell FK506-binding protein, 59kD
  • FKBP59
  • FKBP5952 kDa FK506-binding protein
  • HBI
  • HSP binding immunophilin
  • Hsp56
  • HSP-binding immunophilin
  • Immunophilin FKBP52
  • p52
  • p59
  • peptidyl-prolyl cis-trans isomerase FKBP4
  • peptidylprolyl cis-trans isomerase
  • PPIase FKBP4
  • PPIase
  • rotamase

Background

Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin Abinding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90 (4). Although FKBP52 and FKBP51 share approx. 75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90- binding characteristics (3, 5). Also, whereas FKBP51 typically has a role with the progesterone receptor, FKBP52 has been found to be linked to the progesterone, androgen and glucocorticoid receptors (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-96874AF405
Alexa Fluor 488 Labeled NB110-96874AF488
Alexa Fluor 647 Labeled NB110-96874AF647
Alexa Fluor 700 Labeled NB110-96874AF700
DyLight 350 Labeled NB110-96874UV
DyLight 405 Labeled NB110-96874V
DyLight 488 Labeled NB110-96874G
DyLight 680 Labeled NB110-96874FR
DyLight 755 Labeled NB110-96874IR

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Bioinformatics

Gene Symbol FKBP4
Entrez
Uniprot