ASCC2 Antibody (OTI1E8) - Azide and BSA Free

Images

 
Western Blot: ASCC2 Antibody (OTI1E8) - Azide and BSA Free [NBP2-72273] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASCC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: ASCC2 Antibody (OTI1E8) - Azide and BSA Free [NBP2-72273] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ASCC2.
Flow Cytometry: ASCC2 Antibody (OTI1E8) - Azide and BSA Free [NBP2-72273] - Analysis of Hela cells, using anti-ASCC2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, CyTOF-ready
Clone
OTI1E8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

ASCC2 Antibody (OTI1E8) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human ASCC2 (NP_115580) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ASCC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Western Blot 1:500
Theoretical MW
86.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for ASCC2 Antibody (OTI1E8) - Azide and BSA Free

  • activating signal cointegrator 1 complex subunit 2
  • ASC 1 complex subunit P100
  • ASC-1 complex subunit p100
  • ASC1p100
  • DKFZp586O0223
  • FLJ21588
  • Trip4 complex subunit p100

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ASCC2