SND1 Antibody - BSA Free

Western Blot: SND1 Antibody [NBP2-94469] - Analysis of extracts of various cell lines, using SND1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1s.

Immunocytochemistry/Immunofluorescence: SND1 Antibody [NBP2-94469] - Analysis of L929 cells using SND1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: SND1 Antibody [NBP2-94469] - Analysis of C6 cells using SND1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: SND1 Antibody [NBP2-94469] - Mouse kidney using SND1 Rabbit pAb at dilution of 1:100 (40x lens).

Western Blot: SND1 Antibody [NBP2-94469] - Analysis of extracts from normal (control) and SND1 Rabbit pAb knockout (KO) HeLa cells, using SND1 Rabbit pAb antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunoprecipitation: SND1 Antibody [NBP2-94469] - analysis of 200ug extracts of Jurkat cells using 3ug SND1 antibody. Western blot was performed from the immunoprecipitate using SND1 antibody at a dilition of 1:1000.

Immunohistochemistry: SND1 Antibody - Azide and BSA Free [SND1] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using [KO Validated] SND1 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: SND1 Antibody - Azide and BSA Free [SND1] - Immunohistochemistry analysis of paraffin-embedded Rat colon using [KO Validated] SND1 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Western Blot: SND1 Antibody - Azide and BSA Free [SND1] - Western blot analysis of various lysates, using SND1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

Immunohistochemistry: SND1 Antibody - Azide and BSA Free [SND1] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] SND1 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Western Blot: SND1 Antibody - Azide and BSA Free [SND1] - Western blot analysis of lysates from wild type (WT) and SND1 knockout (KO) HeLa cells, using [KO Validated] SND1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.

Western Blot: SND1 Antibody - Azide and BSA Free [SND1] - Western blot analysis of various lysates using [KO Validated] SND1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SND1 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit SND1 Antibody - BSA Free (NBP2-94469) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-285 of human SND1 (NP_055205.2). MVLSGCAIIVRGQPRGGPPPERQINLSNIRAGNLARRAAATQPDAKDTPDEPWAFPAREFLRKKLIGKEVCFTIENKTPQGREYGMIYLGKDTNGENIAESLVAEGLATRREGMRANNPEQNRLSECEEQAKAAKKGMWSEGNGSHTIRDLKYTIENPRHFVDSHHQKPVNAIIEHVRDGSVVRALLLPDYYLVTVMLSGIKCPTFRREADGSETPEPFAAEAKFFTESRLLQRDVQIILESCHNQNILGTILHPNGNIT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SND1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:500 - 1:1000
  • Knockout Validated
  • Western Blot 1:500-1:2000
• Theoretical MW: 101 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for SND1 Antibody - BSA Free

• 100 kDa coactivator
• EBNA2 coactivator p100
• p100 co-activator
• p100 EBNA2 co-activator
• p100
• staphylococcal nuclease and tudor domain containing 1
• staphylococcal nuclease domain containing 1
• staphylococcal nuclease domain-containing protein 1
• TDRD11EBNA-2 co-activator (100kD)
• Tudor domain-containing protein 11

Background

SND1 functions as a bridging factor between STAT6 and the basal transcription factor. Plays a role in PIM1 regulation of MYB activity. Functions as a transcriptional coactivator for the Epstein-Barr virus nuclear antigen 2 (EBNA2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: SND1