FKBP52/FKBP4 Antibody (Hi52C) [Alexa Fluor® 405]

• Reactivity: Hu, Mu, Rt, Ca, Ha
• Applications: ICC/IF, IHC
• Clone: Hi52C
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

FKBP52/FKBP4 Antibody (Hi52C) [Alexa Fluor® 405] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Synthetic peptide corresponding to the residues of human FKBP52
• Localization: Cytoplasm , Nucleus
• Specificity: Detects approx 52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FKBP4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB110-96874. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for FKBP52/FKBP4 Antibody (Hi52C) [Alexa Fluor® 405]

• 51 kDa FK506-binding protein
• 52 kDa FKBP
• 59 kDa immunophilin
• EC 5.2.1.8
• FK506 binding protein 4, 59kDa
• FK506-binding protein 4 (59kD)
• FK506-binding protein 4
• FKBP4
• FKBP-4
• FKBP51
• FKBP52
• FKBP-52
• FKBP52T-cell FK506-binding protein, 59kD
• FKBP59
• FKBP5952 kDa FK506-binding protein
• HBI
• HSP binding immunophilin
• Hsp56
• HSP-binding immunophilin
• Immunophilin FKBP52
• p52
• p59
• peptidyl-prolyl cis-trans isomerase FKBP4
• peptidylprolyl cis-trans isomerase
• PPIase FKBP4
• PPIase
• rotamase

Background

Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin Abinding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90 (4). Although FKBP52 and FKBP51 share approx. 75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90- binding characteristics (3, 5). Also, whereas FKBP51 typically has a role with the progesterone receptor, FKBP52 has been found to be linked to the progesterone, androgen and glucocorticoid receptors (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FKBP4
• Entrez:
  • 2288(Human)
  • 14228(Mouse)
  • 260321(Rat)
• Uniprot:
  • NP_002005.1(Human)