FGF basic/FGF2/bFGF Antibody [Biotin]

Images

 
FGF basic/FGF2/bFGF was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human FGF basic/FGF2/bFGF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF233) ...read more
FGF basic/FGF2/bFGF was detected in immersion fixed paraffin-embedded sections of human ovarian array using Human FGF basic/FGF2/bFGF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF233) at 10 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations

FGF basic/FGF2/bFGF Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human FGF basic/FGF2/bFGF
Pro143-Ser288
Accession # P09038
Specificity
Detects human FGF basic/FGF2/bFGF in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) FGF acidic and rhFGF-7 is observed under non-reducing conditions.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FGF2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF basic/FGF2/bFGF Antibody [Biotin]

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • HBGF-2
  • heparin-binding growth factor 2
  • Prostatropin

Background

FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

DVE00
Species: Hu
Applications: ELISA
236-EG
Species: Hu
Applications: BA
232-FA/CF
Species: Hu
Applications: BA
291-G1
Species: Hu
Applications: BA
NB600-1287
Species: Ca, Hu, Mu, Rb, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-00932
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
D6050
Species: Hu
Applications: ELISA
210-TA
Species: Hu
Applications: BA
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP1-47842
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
294-HG/CF
Species: Hu
Applications: BA
DBD00
Species: Hu
Applications: ELISA
251-KG/CF
Species: Hu
Applications: BA
NB300-141
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
AF467
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
267-N3/CF
Species: Hu
Applications: BA
256-GF/CF
Species: Hu
Applications: BA
BAF233
Species: Hu
Applications: WB, IHC

Publications for FGF basic/FGF2/bFGF Antibody (BAF233) (0)

There are no publications for FGF basic/FGF2/bFGF Antibody (BAF233).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FGF basic/FGF2/bFGF Antibody (BAF233) (0)

There are no reviews for FGF basic/FGF2/bFGF Antibody (BAF233). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FGF basic/FGF2/bFGF Antibody (BAF233). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Secondary Antibodies

 

Isotype Controls

Additional FGF basic/FGF2/bFGF Products

Bioinformatics Tool for FGF basic/FGF2/bFGF Antibody (BAF233)

Discover related pathways, diseases and genes to FGF basic/FGF2/bFGF Antibody (BAF233). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FGF basic/FGF2/bFGF Antibody (BAF233)

Discover more about diseases related to FGF basic/FGF2/bFGF Antibody (BAF233).
 

Pathways for FGF basic/FGF2/bFGF Antibody (BAF233)

View related products by pathway.

PTMs for FGF basic/FGF2/bFGF Antibody (BAF233)

Learn more about PTMs related to FGF basic/FGF2/bFGF Antibody (BAF233).

Blogs on FGF basic/FGF2/bFGF

There are no specific blogs for FGF basic/FGF2/bFGF, but you can read our latest blog posts.
Download our IHC Handbook

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FGF basic/FGF2/bFGF Antibody [Biotin] and receive a gift card or discount.

Bioinformatics

Gene Symbol FGF2
Uniprot