FGF basic/FGF2/bFGF Antibody [Unconjugated]

FGF basic/FGF2/bFGF was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human FGF basic/FGF2/bFGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑233‑NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Bovine FGF basic/FGF2/bFGF (Catalog # 133-FB) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF basic/FGF2/bFGF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF basic/FGF2/bFGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-233-NA). The ND50 is typically 0.08‑0.4 µg/mL.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

FGF basic/FGF2/bFGF Antibody [Unconjugated] Summary

• Immunogen: Bovine brain-derived FGF basic/FGF2/bFGF
• Specificity: Detects human and bovine FGF basic/FGF2/bFGF in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant mouse FGF basic/FGF2/bFGF is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FGF2
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.08-0.4 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF basic/FGF2/bFGF Antibody [Unconjugated]

• basic fibroblast growth factor bFGF
• Basic fibroblast growth factor
• bFGF
• FGF basic
• FGF2
• FGF-2
• FGFBprostatropin
• fibroblast growth factor 2 (basic)
• HBGF-2
• heparin-binding growth factor 2
• Prostatropin

Background

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35‑60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. A variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron differentiation, survival, and regeneration. FGF basic also modulates embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.

1. Coulier, F. et al. (1997) J. Mol. Evol. 44:43.
2. Chen, C.H. et al. (2004) Curr. Vasc. Pharmacol. 2:33.
3. Mohammadi, M. et al. (2005) Curr. Opin. Struct. Biol. 15:506.
4. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGF basic/FGF2/bFGF Antibody (AF-233-NA)(18)

Publications using AF-233-NA

• S Tiwary, JE Morales, SC Kwiatkowsk, FF Lang, G Rao, JH McCarty Metastatic Brain Tumors Disrupt the Blood-Brain Barrier and Alter Lipid Metabolism by Inhibiting Expression of the Endothelial Cell Fatty Acid Transporter Mfsd2a Sci Rep, 2018;8(1):8267. 2018 [PMID: 29844613] (Neutralization, Human)
• DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2018;15(1):39-46. 2018 [PMID: 29320487] (IHC, Mouse)
• R Somasundar, G Zhang, M Fukunaga-K, M Perego, C Krepler, X Xu, C Wagner, D Hristova, J Zhang, T Tian, Z Wei, Q Liu, K Garg, J Griss, R Hards, M Maurer, C Hafner, M Mayerhöfer, G Karanikas, A Jalili, V Bauer-Pohl, F Weihsengru, K Rappersber, J Koller, R Lang, C Hudgens, G Chen, M Tetzlaff, L Wu, DT Frederick, RA Scolyer, GV Long, M Damle, C Ellingswor, L Grinman, H Choi, BJ Gavin, M Dunagin, A Raj, N Scholler, L Gross, M Beqiri, K Bennett, I Watson, H Schaider, MA Davies, J Wargo, BJ Czerniecki, L Schuchter, D Herlyn, K Flaherty, M Herlyn, SN Wagner Tumor-associated B-cells induce tumor heterogeneity and therapy resistance Nat Commun, 2017;8(1):607. 2017 [PMID: 28928360] (Neutralization, Human)
• Y Li, AM Lucas-Osma, S Black, MV Bandet, MJ Stephens, R Vavrek, L Sanelli, KK Fenrich, AF Di Narzo, S Dracheva, IR Winship, K Fouad, DJ Bennett Pericytes impair capillary blood flow and motor function after chronic spinal cord injury Nat. Med., 2017;0(0):. 2017 [PMID: 28459438] (IHC, Rat)
• Ashki N, Chan A, Qin Y, Wang W, Kiyohara M, Lin L, Braun J, Wadehra M, Gordon L Peroxynitrite upregulates angiogenic factors VEGF-A, BFGF, and HIF-1alpha in human corneal limbal epithelial cells. Invest Ophthalmol Vis Sci, 2014;55(3):1637-46. 2014 [PMID: 24398102] (Western Blot, Human)
• Ferreras, Cristina, Rushton, Graham, Cole, Claire L, Babur, Muhammad, Telfer, Brian A, van Kuppevelt, Toin H, Gardiner, John M, Williams, Kaye J, Jayson, Gordon C, Avizienyte, Egle Endothelial heparan sulfate 6-o-sulfation levels regulate angiogenic responses of endothelial cells to fibroblast growth factor 2 and vascular endothelial growth factor. J Biol Chem, 2102;287(43):36132-46. 2102 [PMID: 22927437] (IHC-Fr, Human)
• Inhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration via production of angiocrine factors in mice. Blood, 2012;119(26):6382-93. 2012 [PMID: 22573404] (In Vivo, Mouse)
• Choi W, Wolber R, Gerwat W The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin. J. Cell. Sci., 2010;123(0):3102-11. 2010 [PMID: 20736300] (Western Blot, Human)
• Suga H, Eto H, Shigeura T, Inoue K, Aoi N, Kato H, Nishimura S, Manabe I, Gonda K, Yoshimura K IFATS Series: FGF-2-induced HGF Secretion By Adipose-Derived Stromal Cells Inhibits Post-Injury Fibrogenesis Through A JNK-Dependent Mechanism. Stem Cells, 2008;0(0):. 2008 [PMID: 18772314] (Neutralization, Mouse)
• Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells. Exp. Cell Res., 2008;314(11):2176-86. 2008 [PMID: 18570917] (Neutralization, Mouse)
• Tomanek RJ, Hansen HK, Christensen LP Temporally expressed PDGF and FGF-2 regulate embryonic coronary artery formation and growth. Arterioscler. Thromb. Vasc. Biol., 2008;28(7):1237-43. 2008 [PMID: 18420995] (Neutralization, Avian)
• Vonlaufen A, Joshi S, Qu C, Phillips PA, Xu Z, Parker NR, Toi CS, Pirola RC, Wilson JS, Goldstein D, Apte MV Pancreatic stellate cells: partners in crime with pancreatic cancer cells. Cancer Res., 2008;68(7):2085-93. 2008 [PMID: 18381413] (Neutralization, Human)
• Tatrai P, Dudas J, Batmunkh E, Máthé M, Zalatnai A, Schaff Z, Ramadori G, Kovalszky I Agrin, a novel basement membrane component in human and rat liver, accumulates in cirrhosis and hepatocellular carcinoma. Lab. Invest., 2006;86(11):1149-60. 2006 [PMID: 16983329] (IHC-Fr, Human)
• Dabrosin C Positive correlation between estradiol and vascular endothelial growth factor but not fibroblast growth factor-2 in normal human breast tissue in vivo. Clin. Cancer Res., 2005;11(22):8036-41. 2005 [PMID: 16299233] (IHC-P, Human)
• Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis. Development, 2005;132(6):1223-34. 2005 [PMID: 15716343] (Neutralization, Mouse)
• Brill A, Elinav H, Varon D Differential role of platelet granular mediators in angiogenesis. Cardiovasc. Res., 2004;63(2):226-35. 2004 [PMID: 15249180] (Neutralization, Bovine)
• Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis. Blood, 2003;102(2):638-45. 2003 [PMID: 12649156] (Neutralization, Human)
• Said EA, Krust B, Nisole S, Svab J, Briand JP, Hovanessian AG The anti-HIV cytokine midkine binds the cell surface-expressed nucleolin as a low affinity receptor. J. Biol. Chem., 2002;277(40):37492-502. 2002 [PMID: 12147681] (ICC, Hamster)

FAQs for FGF basic/FGF2/bFGF Antibody (AF-233-NA). (Showing 1 - 1 of 1 FAQs).

1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
   • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits, but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Bioinformatics

• Gene Symbol: FGF2