Home » FGF-4 » FGF-4 Antibodies » FGF-4 Antibody (26019)

FGF-4 Antibody (26019)

 Submit a Review

Images

 
There are currently no images for FGF-4 Antibody (MAB635).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA(Cap)
Clone
26019
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB635-SP
100% Guarantee on every product

FGF-4 Antibody (26019) Summary

Immunogen
E. coli-derived recombinant human FGF-4
Accession # P08620
Specificity
When used in combination with the biotinylated anti-FGF-4 detection antibody (Catalog # BAF235) in sandwich ELISAs, no significant cross-reactivity or interference was observed with recombinant human (rh) FGF acidic, bFGF acidic, rhFGF basic, bFGF basic, rhFGF-5, rhFGF-6, or rhFGF-7 (KGF).
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FGF4
Purity
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human FGF-4 Biotinylated Antibody (Catalog number BAF235)
Standard: Recombinant Human FGF-4 (Catalog number 235-F4)

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from ascites
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-4 Antibody (26019)

  • FGF4
  • FGF-4
  • fibroblast growth factor 4
  • HBGF-4
  • HBGF-4Transforming protein KS3
  • heparin secretory transforming protein 1
  • Heparin secretory-transforming protein 1
  • Heparin-binding growth factor 4
  • HST-1
  • HST-1HSTF-1
  • HSTF1fibroblast growth factor 4 splice isoform
  • HSTFGF-4
  • human stomach cancer, transforming factor from FGF-related oncogene
  • kaposi sarcoma oncogene
  • KFGF
  • K-FGF
  • KS3
  • oncogene HST

Background

FGF-4 is a member of the FGF family of growth factors. FGF-4 is important in embryonic angiogenesis and limb development and is mitogenic for fibroblasts and endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

FGF basic/FGF2
[Unconjugated]
233-FB
1 µg/lane of Recombinant Human FGF basic/FGF2 (146 aa) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human FGF basic/FGF2 (146 aa) (Catalog # 233-FB) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.1-0.6 ng/mL.
(13 Reviews)
(314 Publications)
Species: Hu
FGF-3 Antibody
(MSD1)
NB200-603
FGF-3 Antibody (MSD1)
(1 Review)
(1 Publication)
Species: Hu, Mu, Xp
Applications: WB, IHC, IHC-Fr, IHC-P, IP, IF
FGF-8
[Unconjugated]
423-F8/CF
Recombinant Human/Mouse FGF-8b (Catalog # 423-F8/CF) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 6.5-40 ng/mL in the presence of 1 μg/mL heparin.
1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.
(42 Publications)
Species: Hu, Mu
Sonic Hedgehog/Shh
[Unconjugated]
1845-SH
1 μg/lane of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
MALDI-TOF analysis of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus. The major peak corresponds to the calculated molecular mass, 19814 Da. The minor peak at 20029  is a matrix-associated artifact of the MALDI-TOF.
Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus (Catalog # 1845-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1‑0.4 μg/mL.
(5 Reviews)
(31 Publications)
Species: Hu
OCT4 Antibody
NB100-2379
Western Blot: OCT4 Antibody [NB100-2379] - WB detection of OCT4 in mouse brain lysate using OCT4 antibody with ECL based detection. The antibody generated a specific band of OCT4 at the expected ~40kDa position.
Western Blot: OCT4 Antibody [NB100-2379] - The expression of pluripotency factors Oct4 in human head and neck cancer cell lines.
Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NB100-2379] - OCT4 staining in NTERA-2 cells detected with a Dylight 488 labeled secondary antibody.
(1 Review)
(11 Publications)
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, ICC/IF, IHC
FGF acidic/FGF1
[Unconjugated]
232-FA
FGF acidic/FGF1 [Unconjugated]
(1 Review)
(17 Publications)
Species: Hu
FGFR2 Antibody
(OTI5C5)
NBP2-00932
Western Blot: FGF R2 Antibody (5C5) [NBP2-00932] - Analysis of FGF R2 in HEK293T cells (left, control) and HEK293T cells overexpressing FGF R2 (right) using anti-FGF R2 antibody. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: FGFR2 Antibody (5C5) [NBP2-00932] Staining of COS7 cells transiently transfected by pCMV6-ENTRY FGFR2.
Immunohistochemistry-Paraffin: FGFR2 Antibody (5C5) [NBP2-00932] - Staining of paraffin-embedded Human endometrium tissue using anti-FGFR2 mouse monoclonal antibody.
(1 Review)
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
BMP-4
[Unconjugated]
314-BP
1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation. 
Recombinant Human BMP-4 (Catalog #314-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 2.5-15 ng/mL.
(3 Reviews)
(321 Publications)
Species: Hu
FGFR1 Antibody
(M19B2)
NB600-1287
Western Blot: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis of MCF7 (A), HepG2 (B), NIH3T3 (C), and PC-12 (D) cell lysate using FGF R1 antibody at 2 ug/ml.
Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis using the Alexa Fluor 488 (R) conjugate of NB600-1287. Staining of FGF R1 in HEK293 and HEK293 FGFR transfected cells using anti-FGF R1 antibody. (Red) Positive cells, (Green) Negative cells, (Blue) Negative cells + antibody, (Pink) Positive cells + antibody. Image from verified customer review.
Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using FGF R1 antibody (Dark Blue) and Isotype control (Orange). An antibody concentration of 1 ug/1x10^6 cells was used.
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IP, CyTOF-ready
HGF [Unconjugated]
294-HG
HGF [Unconjugated]
(4 Reviews)
(79 Publications)
Species: Hu
BMP-2
[Unconjugated]
355-BM/CF
BMP-2 [Unconjugated]
(137 Publications)
Species: Hu, Mu, Rt
FGF-6
6829-F6/CF
FGF-6
Species: Hu
SOX2 Antibody
(245610) [Unconjugate
MAB2018
Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 50 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
E‑Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E‑Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (green; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(12 Reviews)
(107 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-reported, ICC, ICFlow
Cyclin D1 Antibody
(SPM587)
NBP2-32840
Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Western Blot of cyclin D1 in (1) C2C12 (2) HepG2 & (3) NIH3T3 Lysate with Cyclin D1 Ab (SPM587)
Immunohistochemistry-Paraffin: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Formalin-paraffin human Mantle Cell Lymphoma stained with Cyclin D1 Ab (Clone SPM587).
Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - analysis of Cyclin D1 in human breast cancer cell line MDA-MB-231 cell lysate using anti-Cyclin D1. Image from verified customer review.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP
EGF [Unconjugated]
236-EG
Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 20‑100 pg/mL.
1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
(14 Reviews)
(392 Publications)
Species: Hu
KGF/FGF-7
[Unconjugated]
251-KG
KGF/FGF-7 [Unconjugated]
(42 Publications)
Species: Hu
FGF-10
[Unconjugated]
345-FG
1 μg/lane of Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 21 kDa.
Recombinant Human FGF-10 (Catalog # 345-FG) stimulates cell proliferation of the 4MBr‑5 rhesus monkey epithelial cell line. The ED50 for this effect is 20‑100 ng/mL.
(56 Publications)
Species: Hu
Factor IX Antibody
(2C9)
H00002158-M01
Western Blot: Factor IX Antibody (2C9) [H00002158-M01] - Analysis of F9 expression in transfected 293T cell line by F9 monoclonal antibody (M01), clone 2C9.Lane 1: F9 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
Western Blot: Factor IX Antibody (2C9) [H00002158-M01] - Analysis of F9 over-expressed 293 cell line, cotransfected with F9 Validated Chimera RNAi ( Cat # H00002158-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with F9 monoclonal antibody (M01), clone 2C9 (Cat # H00002158-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunoprecipitation: Factor IX Antibody (2C9) [H00002158-M01] - Analysis of F9 transfected lysate using anti-F9 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F9 MaxPab rabbit polyclonal antibody.
Species: Hu
Applications: WB, ELISA, IP, RNAi

Publications for FGF-4 Antibody (MAB635) (0)

There are no publications for FGF-4 Antibody (MAB635).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FGF-4 Antibody (MAB635) (0)

There are no reviews for FGF-4 Antibody (MAB635). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for FGF-4 Antibody (MAB635) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

Additional FGF-4 Products

Bioinformatics Tool for FGF-4 Antibody (MAB635)

Discover related pathways, diseases and genes to FGF-4 Antibody (MAB635). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FGF-4 Antibody (MAB635)

Discover more about diseases related to FGF-4 Antibody (MAB635).
 

Pathways for FGF-4 Antibody (MAB635)

View related products by pathway.

PTMs for FGF-4 Antibody (MAB635)

Learn more about PTMs related to FGF-4 Antibody (MAB635).

Blogs on FGF-4

There are no specific blogs for FGF-4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FGF-4 Antibody (26019) and receive a gift card or discount.


Bioinformatics

Gene Symbol FGF4
Uniprot