Fer Antibody

Images

 
Western Blot: FER Antibody [NBP1-20089] - Jurkat cell lysates.
Immunocytochemistry/ Immunofluorescence: FER Antibody [NBP1-20089] - FER antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.12 mg/ml

Fer Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human FER (within residues 400-450). [Swiss-Prot# P16591]
Localization
Cytoplasm.
Predicted Species
Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FER
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
This FER antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~94 kDa. In ICC/IF cytoplasmic staining was observed.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Jurkat Lysate (NB800-PC2)
Control Peptide
Fer Peptide (NBP1-20089PEP)

Reactivity Notes

Human. Predicted to react with mouse and canine based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.12 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fer Antibody

  • EC 2.7.10
  • EC 2.7.10.2
  • fer (fps/fes related) tyrosine kinase
  • Fer
  • p94-Fer
  • Phosphoprotein NCP94
  • Proto-oncogene c-Fer
  • TYK3
  • TYK3phosphoprotein NCP94
  • Tyrosine kinase 3
  • tyrosine-protein kinase Fer

Background

FER (Feline sarcoma related) is a ubiquitously expressed non-receptor protein tyrosine kinase with about 94-kD size that acts downstream of cell surface receptors for growth factors and plays a key role in regulating actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. It acts downstream of EGFR, KIT, FCER1, PDGFRA/PDGFRB receptor etc and phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1, PTPN11, JUP, PTPN1 and STAT3. Similar to other tyrosine kinases, FER has a central Src-homology 2 (SH2) domain involved in binding to phosphotyrosine-containing peptide sequences and a highly conserved C-terminal kinase domain, whereas it is different by an NH2-terminal FER/ClP4 homology and adjacent coiled-coil domains. FER is expressed ubiquitously in human tissues and higher FER levels are observed in cancer cells compared with adjacent normal cells e.g. renal cell carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for Fer Antibody (NBP1-20089): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fer Antibody (NBP1-20089) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-20089AF405
Alexa Fluor 488 Labeled NBP1-20089AF488
Alexa Fluor 647 Labeled NBP1-20089AF647
Alexa Fluor 700 Labeled NBP1-20089AF700
Biotin Labeled NBP1-20089B
DyLight 405 Labeled NBP1-20089V
DyLight 488 Labeled NBP1-20089G
DyLight 550 Labeled NBP1-20089R
DyLight 650 Labeled NBP1-20089C
FITC Labeled NBP1-20089F
HRP Labeled NBP1-20089H

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Calculators

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Bioinformatics

Gene Symbol FER
Entrez