Jak3 Antibody (5H2)

Images

 
Western Blot: Jak3 Antibody (5H2) [NBP2-37737] - Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1).
Immunocytochemistry/ Immunofluorescence: Jak3 Antibody (5H2) [NBP2-37737] - Confocal immunofluorescence analysis of 3T3-L1 cells using JAK3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 ...read more
Flow Cytometry: Jak3 Antibody (5H2) [NBP2-37737] - Flow cytometric analysis of Hela cells using JAK3 mouse mAb (blue) and negative control (red).
Immunocytochemistry/ Immunofluorescence: Jak3 Antibody (5H2) [NBP2-37737] - Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using JAK3 mouse mAb (green). Red: Actin filaments have been ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
5H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Jak3 Antibody (5H2) Summary

Immunogen
Purified recombinant fragment of human JAK3 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
JAK3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-37737 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Jak3 Antibody (5H2)

  • EC 2.7.10
  • Jak3
  • JAK3_HUMAN
  • JAK-3EC 2.7.10.2
  • JAKL
  • JAKLtyrosine-protein kinase JAK3
  • Janus kinase 3Leukocyte janus kinase
  • LJAK
  • L-JAKJanus kinase 3 (a protein tyrosine kinase, leukocyte)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Jak3 Antibody (NBP2-37737)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Mouse
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Jak3 Antibody (NBP2-37737) (0)

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Secondary Antibodies

 

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Blogs on Jak3.

Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.

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Calculators

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Bioinformatics

Gene Symbol JAK3