Fer Antibody [FITC]

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Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

Fer Antibody [FITC] Summary

Immunogen
Synthetic peptide made to an internal portion of human FER (within residues 400-450). [Swiss-Prot# P16591]
Localization
Cytoplasm.
Predicted Species
Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FER
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
Application Notes
This FER antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~94 kDa. In ICC/IF cytoplasmic staining was observed.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Predicted to react with mouse and canine based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Fer Antibody [FITC]

  • EC 2.7.10
  • EC 2.7.10.2
  • fer (fps/fes related) tyrosine kinase
  • Fer
  • p94-Fer
  • Phosphoprotein NCP94
  • Proto-oncogene c-Fer
  • TYK3
  • TYK3phosphoprotein NCP94
  • Tyrosine kinase 3
  • tyrosine-protein kinase Fer

Background

FER (Feline sarcoma related) is a ubiquitously expressed non-receptor protein tyrosine kinase with about 94-kD size that acts downstream of cell surface receptors for growth factors and plays a key role in regulating actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. It acts downstream of EGFR, KIT, FCER1, PDGFRA/PDGFRB receptor etc and phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1, PTPN11, JUP, PTPN1 and STAT3. Similar to other tyrosine kinases, FER has a central Src-homology 2 (SH2) domain involved in binding to phosphotyrosine-containing peptide sequences and a highly conserved C-terminal kinase domain, whereas it is different by an NH2-terminal FER/ClP4 homology and adjacent coiled-coil domains. FER is expressed ubiquitously in human tissues and higher FER levels are observed in cancer cells compared with adjacent normal cells e.g. renal cell carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FER
Entrez