FDX1 Antibody - BSA Free

Immunohistochemistry-Paraffin: FDX1 Antibody [NBP1-89227] - Analysis in human adrenal gland and cerebral cortex tissues using NBP1-89227 antibody. Corresponding FDX1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: FDX1 Antibody [NBP1-89227] - Staining of human adrenal gland, cerebral cortex, endometerium and tonsil using Anti-FDX1 antibody NBP1-89227 (A) shows similar protein distribution across tissues to independent antibody (B).

Western Blot: FDX1 Antibody [NBP1-89227] - Analysis in control (vector only transfected HEK293T lysate) and FDX1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: FDX1 Antibody [NBP1-89227] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.

Immunohistochemistry-Paraffin: FDX1 Antibody [NBP1-89227] - Staining of human tonsil shows no positivity in germinal center cells as expected.

Immunohistochemistry-Paraffin: FDX1 Antibody [NBP1-89227] - Staining of human adrenal gland shows strong granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: FDX1 Antibody [NBP1-89227] - Staining of human endometrium shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: FDX1 Antibody [NBP1-89227] - Staining of human cerebral cortex shows no positivity in neuronal cells as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

FDX1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: NLDIDGFGACEGTLACSTCHLIFEDHIYEKLDAITDEENDMLDLAYGLTDRSRLGC
• Predicted Species: Mouse (98%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FDX1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  FDX1 Protein (NBP1-89227PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for FDX1 Antibody - BSA Free

• Adrenal ferredoxin
• adrenodoxin, mitochondrial
• ADXhepatoredoxin
• FDX
• ferredoxin 1
• ferredoxin-1
• Hepatoredoxin
• LOH11CR1D
• mitochondrial adrenodoxin

Background

The product of this gene is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450. This particular oxidation/reduction system is found in steroidogenic tissues, and is involved with the synthesis of bile acid and vitamin D. In addition to the expressed gene at this chromosomal locus (11q22), there are pseudogenes located on chromosomes 20 and 21. This gene product has been identified in a number of different tissues but all forms have been shown to be identical and are not tissue specific. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FDX1 Antibody (NBP1-89227)(1)

Publication using NBP1-89227

• Wang K, Wang M, Li Z et al. Systematic analysis of the cuprotosis in tumor microenvironment and prognosis of gastric cancer Heliyon 2023-03-01 [PMID: 36895378] (IHC, Human)

Bioinformatics

• Gene Symbol: FDX1