| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89227-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38946-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22548-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38619-20ul | |||
![Immunohistochemistry-Paraffin: FDX1 Antibody [NBP1-89227] - Analysis in human adrenal gland and cerebral cortex tissues using NBP1-89227 antibody. Corresponding FDX1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FDX1-Antibody-Immunohistochemistry-Paraffin-NBP1-89227-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FDX1 Antibody [NBP1-89227] - Analysis in human adrenal gland and cerebral cortex tissues using NBP1-89227 antibody. Corresponding FDX1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: FDX1 Antibody [NBP1-89227] - Staining of human adrenal gland, cerebral cortex, endometerium and tonsil using Anti-FDX1 antibody NBP1-89227 (A) shows similar protein distribution across tissues to independent antibody (B).](http://images.novusbio.com/fullsize/FDX1-Antibody-Immunohistochemistry-Paraffin-NBP1-89227-img0015.jpg)
![Western Blot: FDX1 Antibody [NBP1-89227] - Analysis in control (vector only transfected HEK293T lysate) and FDX1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/FDX1-Antibody-Western-Blot-NBP1-89227-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: FDX1 Antibody [NBP1-89227] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.](http://images.novusbio.com/fullsize/FDX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89227-img0006.jpg)
![Immunohistochemistry-Paraffin: FDX1 Antibody [NBP1-89227] - Staining of human tonsil shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/FDX1-Antibody-Immunohistochemistry-Paraffin-NBP1-89227-img0019.jpg)
![Immunohistochemistry-Paraffin: FDX1 Antibody [NBP1-89227] - Staining of human adrenal gland shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/FDX1-Antibody-Immunohistochemistry-Paraffin-NBP1-89227-img0016.jpg)
![Immunohistochemistry-Paraffin: FDX1 Antibody [NBP1-89227] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/FDX1-Antibody-Immunohistochemistry-Paraffin-NBP1-89227-img0017.jpg)
![Immunohistochemistry-Paraffin: FDX1 Antibody [NBP1-89227] - Staining of human cerebral cortex shows no positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/FDX1-Antibody-Immunohistochemistry-Paraffin-NBP1-89227-img0018.jpg)
![Immunohistochemistry-Paraffin: FDX1 Antibody [NBP2-38946] - Staining in human adrenal gland and cerebral cortex tissues using anti-FDX1 antibody. Corresponding FDX1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FDX1-Antibody-Immunohistochemistry-Paraffin-NBP2-38946-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FDX1 Antibody [NBP2-38946] - Staining in human adrenal gland and cerebral cortex tissues using anti-FDX1 antibody. Corresponding FDX1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: FDX1 Antibody [NBP2-38946] - Staining of human adrenal gland, cerebral cortex, colon and lymph node using Anti-FDX1 antibody NBP2-38946 (A) shows similar protein distribution across tissues to independent antibody NBP1-89227 (B).](http://images.novusbio.com/fullsize/FDX1-Antibody-Immunohistochemistry-Paraffin-NBP2-38946-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: FDX1 Antibody [NBP2-38946] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/FDX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38946-img0004.jpg)
![Immunohistochemistry-Paraffin: FDX1 Antibody [NBP2-38946] - Staining of human lymph node.](http://images.novusbio.com/fullsize/FDX1-Antibody-Immunohistochemistry-Paraffin-NBP2-38946-img0011.jpg)
![Immunohistochemistry-Paraffin: FDX1 Antibody [NBP2-38946] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/FDX1-Antibody-Immunohistochemistry-Paraffin-NBP2-38946-img0006.jpg)
![Immunohistochemistry-Paraffin: FDX1 Antibody [NBP2-38946] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/FDX1-Antibody-Immunohistochemistry-Paraffin-NBP2-38946-img0007.jpg)
![Immunohistochemistry-Paraffin: FDX1 Antibody [NBP2-38946] - Staining of human colon.](http://images.novusbio.com/fullsize/FDX1-Antibody-Immunohistochemistry-Paraffin-NBP2-38946-img0010.jpg)
![Immunohistochemistry: FDX1 Antibody (JE63-56) [NBP3-32006] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-FDX1 antibody (NBP3-32006) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32006) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32006_rabbit-fdx1-mab-je63-56-298202413585557.jpg "Immunohistochemistry: FDX1 Antibody (JE63-56) [NBP3-32006] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-FDX1 antibody (NBP3-32006) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32006) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: FDX1 Antibody (JE63-56) [NBP3-32006] - Immunocytochemistry analysis of PC-12 cells labeling FDX1 with Rabbit anti-FDX1 antibody (NBP3-32006) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FDX1 antibody (NBP3-32006) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32006_rabbit-fdx1-mab-je63-56-2920241192332.jpg)
![Western Blot: FDX1 Antibody (JE63-56) [NBP3-32006] - Western blot analysis of FDX1 on different lysates with Rabbit anti-FDX1 antibody (NBP3-32006) at 1/500 dilution. Lane 1: SK-Br-3 cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: HepG2 cell lysate (15 ug/Lane) Lane 4: 293T cell lysate (15 ug/Lane) Predicted band size: 19 kDa Observed band size: 13 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32006) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32006_rabbit-fdx1-mab-je63-56-3920248453525.jpg)
![Western Blot: FDX1 Antibody (SR1148) [NBP3-22548] - Western blot analysis using NBP3-22548 in A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22548_rabbit-fdx1-mab-sr1148-5720231649427.jpg "Western Blot: FDX1 Antibody (SR1148) [NBP3-22548] - Western blot analysis using NBP3-22548 in A549 cell lysate.")
![Western Blot: FDX1 Overexpression Lysate (Adult Normal) [NBL1-10670] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FDX1.](http://images.novusbio.com/fullsize/FDX1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10670-img0002.jpg "Western Blot: FDX1 Overexpression Lysate (Adult Normal) [NBL1-10670] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FDX1.")
![Western Blot: FDX1 Antibody [NBP3-38619] - Western blot analysis of various lysates using FDX1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38619_rabbit-fdx1-pab-1012202411592873.jpg "Western Blot: FDX1 Antibody [NBP3-38619] - Western blot analysis of various lysates using FDX1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.")
![Western Blot: FDX1 Antibody (1G9) [H00002230-M03] - Detection against Immunogen (36.63 KDa) .](http://images.novusbio.com/fullsize/FDX1-Antibody-1G9-Western-Blot-H00002230-M03-img0001.jpg "Western Blot: FDX1 Antibody (1G9) [H00002230-M03] - Detection against Immunogen (36.63 KDa) .")
![Sandwich ELISA: FDX1 Antibody (4G5) [H00002230-M02] - Detection limit for recombinant GST tagged FDX1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FDX1-Antibody-4G5-Sandwich-ELISA-H00002230-M02-img0001.jpg "Sandwich ELISA: FDX1 Antibody (4G5) [H00002230-M02] - Detection limit for recombinant GST tagged FDX1 is 0.1 ng/ml as a capture antibody.")
![Sandwich ELISA: FDX1 Antibody (1E7) [H00002230-M01] - Detection limit for recombinant GST tagged FDX1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FDX1-Antibody-1E7-Sandwich-ELISA-H00002230-M01-img0001.jpg "Sandwich ELISA: FDX1 Antibody (1E7) [H00002230-M01] - Detection limit for recombinant GST tagged FDX1 is 0.03 ng/ml as a capture antibody.")
![SDS-Page: Recombinant Human FDX1 Protein [NBP1-50829]](http://images.novusbio.com/fullsize/FDX1-Protein-SDS-Page-NBP1-50829-img0001.jpg "SDS-Page: Recombinant Human FDX1 Protein [NBP1-50829]")