Fc gamma RIIIA/CD16a Antibody (3G8)

Images

 
Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) purified / GAM-APC.
Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC-CyTM7.
Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE.
Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE-CyTM7.
Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PerCP.
Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PerC-CyTM5.5
Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) Pacific BlueTM.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP, CyTOF-ready
Clone
3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Fc gamma RIIIA/CD16a Antibody (3G8) Summary

Immunogen
Human neutrophils.
Specificity
The mouse monoclonal antibody 3G8 recognizes CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils). HLDA V; WS Code NK80
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FCGR3A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 6 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
Immunohistochemistry-Frozen sections: acetone fixation recommended Functional: In vitro Stimulation of NK cell proliferation, blocking of IgG binding and phagocytosis, inhibition of cytotoxic activity, in vivo NK cell depletion

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Fc gamma RIIIA/CD16a Antibody (3G8)

  • CD16a antigen
  • CD16a
  • CD16FCRIIIA
  • Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
  • Fc fragment of IgG, low affinity IIIa, receptor for (CD16)
  • Fc gamma receptor III-A
  • Fc gamma RIIIA
  • FCG3
  • Fc-gamma receptor III-2 (CD 16)
  • Fc-gamma receptor IIIb (CD16)
  • Fc-gamma RIII
  • Fc-gamma RIIIa
  • Fc-gamma RIII-alpha
  • FCGR3
  • FCGR3A
  • FCGRIII
  • FcgRIIIA
  • FcR-10
  • FCRIII
  • FcRIIIa
  • IGFR3FcgammaRIIIA
  • immunoglobulin G Fc receptor III
  • low affinity III, receptor for (CD16)
  • low affinity immunoglobulin gamma Fc region receptor III-A
  • neutrophil-specific antigen NA

Background

CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for Fc gamma RIIIA/CD16a Antibody (NBP1-50056). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in developing a surrogate ADCC assay (FC gamma III a binding assay) for which, I would require a CD16 Recombinant Protein as an antigen and Anti-Human IgG (Fc specific) as a secondary antibody as I am planning to perform this assay in an ELISA format. With this brief description, could you please suggest me the right CD16 Recombinant Protein among the three listed products in your web site (http://www.novusbio.com/proteins/cd16). Appreciate if you can share a reference article, where the use of this protein is cited.
    • The right CD16 protein for you depends on what sort of functionality you require for your assay. H00002214-P01 is made by a Taiwanese company called Abnova and we distribute it for them. They do not test the activity of their recombinant proteins. The proteins are produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-transnational modifications they might undergo in an endogenous cell. Because of this, we do not recommend this protein for use in functional assays and do not guarantee them for this use. They are mainly intended for use in Western Blots and ELISA as a positive control with their antibody. NBP1-98908 is produced in E. coli and contains a His-tag and it also not guaranteed for functionality but may be due to its E. coli expression system. H00002214-G01 is also an Abnova and is made in their cell-free wheat germ expression system with proprietary liposome technology. This is a recently developed system by them and is intended to help the proteins fold and maintain functionality. Which protein would be best for you depends on what you require for your assay. Here is a link to our antibodies to the Fc region of human IgG. The best antibody for you will depend on what you would like to use for detection.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol FCGR3A
Entrez