Fc gamma RII/CD32 Antibody [Unconjugated]

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Fc gamma RII/CD32 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Fc gamma RII/CD32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1330) at 15 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Fc gamma RII/CD32 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fc gamma RII/CD32
Ala46-Pro217
Accession # P31994
Specificity
Detects human Fc gamma RII/CD32 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Fc gamma RIIA is observed, and approximately 10% cross-reactivity with recombinant mouse Fc gamma RIIB is observed, and less than 1% cross-reactivity with rhFc gamma RIIIA is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FCGR2A
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF1330 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RII/CD32 Antibody [Unconjugated]

  • CD32 antigen
  • CD32B
  • CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
  • CDw32
  • Fc fragment of IgG, low affinity IIb, receptor (CD32)
  • Fc fragment of IgG, low affinity IIb, receptor for (CD32)
  • Fc gamma RII
  • Fc gamma RIIb
  • FCG2
  • Fc-gamma RII-b
  • fc-gamma-RIIb
  • FCGR2
  • FcgRII
  • fcRII-b
  • IGFR2
  • IgG Fc receptor II-b
  • low affinity immunoglobulin gamma Fc region receptor II-b

Background

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8‑10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6‑10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, FcR gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIB, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three distinct genes encode the human CD32 group, and the protein products are designated Fc gamma  RIIA, B, and C (1). These receptors are glycoproteins of approximately 40 kDa having two extracellular Ig-like domains. The Fc gamma  RII proteins share 94‑99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. Fc gamma  RIIA associates with FcR gamma, and delivers an activating signal upon ligand binding (3, 5). In contrast, Fc gamma  RIIB delivers an inhibitory signal. Fc gamma  RIIC represents an unequal cross-over event between the IIA and IIB genes. Its extracellular domain shares 99% amino acid identity with Fc gamma  RIIB, but a portion of the cytoplasmic domain is closely related to Fc gamma  RIIA. Fc gamma  RII proteins are expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin.

  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fc gamma RII/CD32 Antibody (AF1330)(9)

We have publications tested in 2 confirmed species: Human, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 4 applications: Blocking, Flow, Neut, WB.


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Blocking
(1)
Flow
(1)
Neut
(6)
WB
(1)
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Human
(8)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
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Showing Publications 1 - 9 of 9.
Publications using AF1330 Applications Species
OS Qureshi, TF Rowley, F Junker, SJ Peters, S Crilly, J Compson, A Eddleston, H Björkelund, K Greenslade, M Parkinson, NL Davies, R Griffin, TL Pither, K Cain, L Christodou, L Staelens, E Ward, J Tibbitts, A Kiessling, B Smith, FR Brennan, M Malmqvist, F Fallah-Ara, DP Humphreys Multivalent Fc?-receptor engagement by a hexameric Fc-fusion protein triggers Fc?-receptor internalisation and modulation of Fc?-receptor functions Sci Rep, 2017;7(1):17049. 2017 [PMID: 29213127] (Flow,, Human) Flow Human
Farrukh T Awan Therapeutic CD94/NKG2A blockade improves natural killer cell dysfunction in chronic lymphocytic leukemia Oncoimmunology, 2016;5(10):e1226720. 2016 [PMID: 27853650] (Neut,, Human) Neut Human
Kim Y, Ryu J, Ryu M, Lim S, Han K, Lim I, Han K C-reactive protein induces G2/M phase cell cycle arrest and apoptosis in monocytes through the upregulation of B-cell translocation gene 2 expression. FEBS Lett, 2014;588(4):625-31. 2014 [PMID: 24440351] (Neut,, Human) Neut Human
Emmerich, Petra, Mika, Angela, Schmitz, Herbert Detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (ICB) ELISA. PLoS Negl Trop Dis, 2013;7(12):e2580. 2013 [PMID: 24386498] (WB,, Human) WB Human
He X, Li D, Luo Z, Liang H, Peng H, Zhao Y, Wang N, Liu D, Qin C, Wei Q, Yan H, Shao Y Compromised NK cell-mediated antibody-dependent cellular cytotoxicity in chronic SIV/SHIV infection. PLoS ONE, 2013;8(2):e56309. 2013 [PMID: 23424655] (Neut,, Primate - Macaca mulatta (Rhesus Macaque)) Neut Primate - Macaca mulatta (Rhesus Macaque)
Shin M, Kang Y, Lee N, Wahl E, Kim S, Kang K, Lazova R, Kang I Self double-stranded (ds)DNA induces IL-1beta production from human monocytes by activating NLRP3 inflammasome in the presence of anti-dsDNA antibodies. J Immunol, 2013;190(4):1407-15. 2013 [PMID: 23315075] (Blocking,, Human) Blocking Human
Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes. Diabetologia, 2011;54(10):2713-23. 2011 [PMID: 21744073] (Neut,, Human) Neut Human
Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes. J. Leukoc. Biol., 2008;84(4):1109-19. 2008 [PMID: 18591415] (Neut,, Human) Neut Human
Nabata A, Kuroki M, Ueba H, Hashimoto S, Umemoto T, Wada H, Yasu T, Saito M, Momomura S, Kawakami M C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque. Atherosclerosis, 2007;196(1):129-35. 2007 [PMID: 17531242] (Neut,, Human) Neut Human

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Bioinformatics

Gene Symbol FCGR2A
Entrez
Uniprot