Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein, CF


Recombinant Human Fc gamma RIIB/C (CD32b/c) (Catalog # 1875-CD) binds human IgG with an estimated Kd<200 nM.
1 μg/lane of Recombinant Human Fc gamma RIIB/C (CD32b/c) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing multiple bands at 27-35 kDa.

Product Details

Reactivity HuSpecies Glossary
Applications Binding Activity

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Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein, CF Summary

Details of Functionality
Measured by its ability to bind human IgG with an estimated Kd <200 nM.
Mouse myeloma cell line, NS0-derived human Fc gamma RIIB/C (CD32b/c) protein
Ala46-Pro217, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Binding Activity
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
25-35 kDa, reducing conditions
Read Publications using
1875-CD in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein, CF

  • CD32;CD32B;FCG2;FcgammaRIIb;FCGR2;FCGR2B;FCGR2C;FcGRIIB;FcRII-c;IGFR2;Low affinity immunoglobulin gamma Fc region receptor II-b
  • Fc gamma RIIB/C (CD32b/c)


Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, FcR gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIB, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three distinct genes encode the human CD32 group, and the protein products are designated Fc gamma  RIIA, B, and C (1). These receptors are glycoproteins of approximately 40 kDa having two extracellular Ig-like domains. The Fc gamma  RII proteins share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. Fc gamma  RIIA associates with FcR gamma , and delivers an activating signal upon ligand binding (3, 5). In contrast, Fc gamma  RIIB delivers an inhibitory signal. Fc gamma  RIIC represents an unequal cross-over event between the IIA and IIB genes. Its extracellular domain shares 99% amino acid identity with Fc gamma  RIIB, but a portion of the cytoplasmic domain is closely related to Fc gamma  RIIA. Fc gamma  RII proteins are expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin.

  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.

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Publications for Fc gamma RIIB/C (CD32b/c) (1875-CD)(20)

We have publications tested in 2 confirmed species: Human, Porcine.

We have publications tested in 5 applications: Bioassay, ELISA (Capture), ELISA Capture, Surface Plasmon Resonance, Surface Plasmon Resonance (SPR.

Filter By Application
ELISA (Capture)
ELISA Capture
Surface Plasmon Resonance
Surface Plasmon Resonance (SPR
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using 1875-CD Applications Species
Adhikari, EH;Lu, P;Kang, YJ;McDonald, AR;Pruszynski, JE;Bates, TA;McBride, SK;Trank-Greene, M;Tafesse, FG;Lu, LL; Diverging maternal and infant cord antibody functions from SARS-CoV-2 infection and vaccination in pregnancy bioRxiv : the preprint server for biology 2023-05-02 [PMID: 37205338] (Bioassay, Human) Bioassay Human
TA Bates, P Lu, YJ Kang, D Schoen, M Thornton, SK McBride, C Park, D Kim, WB Messer, ME Curlin, FG Tafesse, LL Lu BNT162b2-induced neutralizing and non-neutralizing antibody functions against SARS-CoV-2 diminish with age Cell Reports, 2022-10-05;41(4):111544. 2022-10-05 [PMID: 36252569] (Bioassay, Human) Bioassay Human
SB An, BG Yang, G Jang, DY Kim, J Kim, SM Oh, N Oh, S Lee, JY Moon, JA Kim, JH Kim, YJ Song, HW Hyun, J Kim, K Lee, D Lee, MJ Kwak, BK Kim, YK Park, CP Hong, JH Kim, HS Lim, MS Ryu, HT Jin, SW Lee, YS Chang, HS Park, YC Sung, MH Jang Combined IgE neutralization and Bifidobacterium longum supplementation reduces the allergic response in models of food allergy Nature Communications, 2022-09-27;13(1):5669. 2022-09-27 [PMID: 36167830] (Bioassay, Human) Bioassay Human
TA Bates, P Lu, YJ Kang, D Schoen, M Thornton, SK McBride, C Park, D Kim, WB Messer, ME Curlin, FG Tafesse, LL Lu BNT162b2 induced neutralizing and non-neutralizing antibody functions against SARSCoV-2 diminish with age medRxiv : the preprint server for health sciences, 2022-08-16;0(0):. 2022-08-16 [PMID: 36032979] (Bioassay, Human) Bioassay Human
A Rogel, FM Ibrahim, SM Thirdborou, F Renart-Dep, CN Birts, SL Buchan, X Preville, EV King, A Al-Shamkha Fc-gamma receptor-mediated crosslinking co-defines the immunostimulatory activity of anti-human CD96 antibodies JCI Insight, 2022-10-10;0(0):. 2022-10-10 [PMID: 35998045] (Bioassay, Human) Bioassay Human
Y Kitanaga, D Yamajuku, S Kubo, K Nakamura, M Maeda, M Seki, Y Kaneko, F Kinugasa, T Morokata, Y Kondo, H Yoshinari, S Nakayamada, T Sumida, Y Tanaka Discovery of a novel Igbeta and FcgammaRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosus International immunopharmacology, 2021-11-12;101(0):108343. 2021-11-12 [PMID: 34781122] (ELISA Capture, Human) ELISA Capture Human
EH Aitken, T Damelang, A Ortega-Paj, A Alemu, W Hasang, S Dini, HW Unger, M Ome-Kaius, MA Nielsen, A Salanti, J Smith, S Kent, PM Hogarth, BD Wines, JA Simpson, A Chung, SJ Rogerson Developing a multivariate prediction model of antibody features associated with protection of malaria-infected pregnant women from placental malaria Elife, 2021-06-29;10(0):. 2021-06-29 [PMID: 34181872] (Bioassay, Human) Bioassay Human
B Vanhove, O Duvaux, J Rousse, PJ Royer, G Evanno, C Ciron, E Lheriteau, L Vacher, N Gervois, R Oger, Y Jacques, S Conchon, A Salama, R Duchi, I Lagutina, A Perota, P Delahaut, M Ledure, M Paulus, RT So, CK Mok, R Bruzzone, M Bouillet, S Brouard, E Cozzi, C Galli, D Blanchard, JM Bach, JP Soulillou High neutralizing potency of swine glyco-humanized polyclonal antibodies against SARS-CoV-2 European Journal of Immunology, 2021-03-02;0(0):. 2021-03-02 [PMID: 33576494] (Bioassay, Porcine) Bioassay Porcine
L Zekri, F Vogt, L Osburg, S Müller, J Kauer, T Manz, M Pflügler, A Maurer, JS Heitmann, I Hagelstein, M Märklin, S Hörner, T Todenhöfer, C Calaminus, A Stenzl, B Pichler, C la Fougère, MA Schneider, HG Rammensee, L Zender, B Sipos, HR Salih, G Jung An IgG-based bispecific antibody for improved dual targeting in PSMA-positive cancer Embo Molecular Medicine, 2020-12-29;0(0):e11902. 2020-12-29 [PMID: 33372710] (ELISA Capture, Human) ELISA Capture Human
PA Blundell, D Lu, M Wilkinson, A Dell, S Haslam, RJ Pleass Insertion of N-Terminal Hinge Glycosylation Enhances Interactions of the Fc Region of Human IgG1 Monomers with Glycan-Dependent Receptors and Blocks Hemagglutination by the Influenza Virus J. Immunol., 2019-01-25;0(0):. 2019-01-25 [PMID: 30683699] (ELISA (Capture), Human) ELISA (Capture) Human
Show All 20 Publications.

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Gene Symbol FCGR2B