Fc gamma RIIIA/CD16a Antibody (3G8) [HRP]

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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications IHC, IHC-Fr
Clone
3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP

Order Details

Fc gamma RIIIA/CD16a Antibody (3G8) [HRP] Summary

Immunogen
Human neutrophils.
Specificity
The mouse monoclonal antibody 3G8 recognizes CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils). HLDA V; WS Code NK80
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FCGR3A
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Fc gamma RIIIA/CD16a Antibody (3G8) [HRP]

  • CD16a antigen
  • CD16a
  • CD16FCRIIIA
  • Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
  • Fc fragment of IgG, low affinity IIIa, receptor for (CD16)
  • Fc gamma receptor III-A
  • Fc gamma RIIIA
  • FCG3
  • Fc-gamma receptor III-2 (CD 16)
  • Fc-gamma receptor IIIb (CD16)
  • Fc-gamma RIII
  • Fc-gamma RIIIa
  • Fc-gamma RIII-alpha
  • FCGR3
  • FCGR3A
  • FCGRIII
  • FcgRIIIA
  • FcR-10
  • FCRIII
  • FcRIIIa
  • IGFR3FcgammaRIIIA
  • immunoglobulin G Fc receptor III
  • low affinity III, receptor for (CD16)
  • low affinity immunoglobulin gamma Fc region receptor III-A
  • neutrophil-specific antigen NA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Fc gamma RIIIA/CD16a Antibody (NBP1-50056H). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in developing a surrogate ADCC assay (FC gamma III a binding assay) for which, I would require a CD16 Recombinant Protein as an antigen and Anti-Human IgG (Fc specific) as a secondary antibody as I am planning to perform this assay in an ELISA format. With this brief description, could you please suggest me the right CD16 Recombinant Protein among the three listed products in your web site (http://www.novusbio.com/proteins/cd16). Appreciate if you can share a reference article, where the use of this protein is cited.
    • The right CD16 protein for you depends on what sort of functionality you require for your assay. H00002214-P01 is made by a Taiwanese company called Abnova and we distribute it for them. They do not test the activity of their recombinant proteins. The proteins are produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-transnational modifications they might undergo in an endogenous cell. Because of this, we do not recommend this protein for use in functional assays and do not guarantee them for this use. They are mainly intended for use in Western Blots and ELISA as a positive control with their antibody. NBP1-98908 is produced in E. coli and contains a His-tag and it also not guaranteed for functionality but may be due to its E. coli expression system. H00002214-G01 is also an Abnova and is made in their cell-free wheat germ expression system with proprietary liposome technology. This is a recently developed system by them and is intended to help the proteins fold and maintain functionality. Which protein would be best for you depends on what you require for your assay. Here is a link to our antibodies to the Fc region of human IgG. The best antibody for you will depend on what you would like to use for detection.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-50056AF350
Alexa Fluor 405 NBP1-50056AF405
Alexa Fluor 488 NBP1-50056AF488
Alexa Fluor 532 NBP1-50056AF532
Alexa Fluor 594 NBP1-50056AF594
Alexa Fluor 647 NBP1-50056AF647
Alexa Fluor 700 NBP1-50056AF700
Alexa Fluor 750 NBP1-50056AF750
Allophycocyanin NBP1-50056APC
Allophycocyanin/Cy7 NBP1-50056APCCY7
Biotin NBP1-50056B
DyLight 350 NBP1-50056UV
DyLight 405 NBP1-50056V
DyLight 488 NBP1-50056G
DyLight 550 NBP1-50056R
DyLight 594 NBP1-50056DL594
DyLight 650 NBP1-50056C
DyLight 680 NBP1-50056FR
DyLight 755 NBP1-50056IR
FITC NBP1-50056F
HRP NBP1-50056H
Janelia Fluor 549 NBP1-50056JF549
Janelia Fluor 646 NBP1-50056JF646
PE NBP1-50056PE
PE/Atto594 NBP1-50056PEATT594
PE/Cy5.5 NBP1-50056PECY55
PE/Cy7 NBP1-50056PECY7
PerCP NBP1-50056PCP

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Bioinformatics

Gene Symbol FCGR3A