Recombinant Human Fc gamma RI/CD64 Protein, CF

Images

 
When Human IgG is coated at 0.5 µg/mL, Recombinant Human Fc gamma RI/CD64 binds with an estimated Kd <0.15 nM.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human Fc gamma RI/CD64 Protein, CF Summary

Details of Functionality
Measured by its ability to bind human IgG with an estimated Kd <0.15 nM.
Source
Mouse myeloma cell line, NS0-derived human Fc gamma RI/CD64 protein
Gln16-Pro288, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained: Gln16 predicted
Protein/Peptide Type
Recombinant Proteins
Gene
FCGR1A
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
45-55 kDa, reducing conditions
Publications
Read Publications using
1257-FC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fc gamma RI/CD64 Protein, CF

  • CD64 antigen
  • CD64
  • Fc fragment of IgG, high affinity Ia, receptor (CD64)
  • Fc gamma RI
  • FCG1
  • Fc-gamma receptor I B2
  • Fc-gamma RI
  • Fc-gamma RIA
  • FcgammaRIa
  • FCGR1
  • FcgRI
  • FCRI
  • FLJ18345
  • high affinity Ia, receptor for (CD64)
  • high affinity immunoglobulin gamma Fc receptor I
  • IgG Fc receptor I

Background

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors
(~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three highly homologous genes (A, B, and C) sharing 98% identity at the nucleotide level have been identified for the human CD64 group (1). Fc gamma  RI is transmembrane protein with three extracellular Ig-like domains, and it delivers an activating signal via the associated Fc R gamma accessory chain. The genes for Fc gamma  RIB and Fc gamma  RIC contain stop codons within their membrane proximal Ig-like domains indicating possible secreted receptors (1, 6). An mRNA splice variant of Fc gamma RIB has a deletion of the membrane-proximal Ig-like domain and encodes a putative transmembrane receptor (6). The high affinity recognition of IgG by Fc gamma  RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses (2). Fc gamma  RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils (1, 4). Its expression is up-regulated during bacterial infections and sepsis.

  1. Van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.
  6. Ernst, L. et al. (1998) Mol Immunol. 35:943.

Customers Who Viewed This Item Also Viewed...

NBP1-27946
Species: Mu
Applications: Flow, IHC-Fr, IP, B/N
MAB2546
Species: Hu
Applications: Flow, CyTOF-ready
NB100-65338
Species: Hu, Po, Ca, Pm
Applications: Flow, IHC, IHC-Fr, IP
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
7268-CT
AF1330
Species: Hu
Applications: WB, Flow, Block, CyTOF-ready, ICC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-04598
Species: Hu
NB110-89474
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Single Cell Western
NBP2-66200
Species: Hu
7270-IL/CF
Species: Hu
AF1730
Species: Hu
Applications: Flow, AdBlk, CyTOF-ready, ICC

Publications for Fc gamma RI/CD64 (1257-FC)(12)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 4 applications: Binding Assay, Bioassay, SPR, Surface Plasmon Resonance.


Filter By Application
Binding Assay
(1)
Bioassay
(6)
SPR
(1)
Surface Plasmon Resonance
(4)
All Applications
Filter By Species
Human
(8)
N/A
(3)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 1257-FC Applications Species
T Oshima, H Miyashita, Y Ishimura, Y Ito, Y Tanaka, A Hori, T Kokubo, T Kurokawa Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey PLoS ONE, 2018;13(5):e0196422. 2018 [PMID: 29723247] (Bioassay) Bioassay
KPM Geuijen, C Oppers-Tie, DF Egging, PJ Simons, L Boon, RBM Schasfoort, MHM Eppink Rapid screening of IgG quality attributes - effects on Fc receptor binding FEBS Open Bio, 2017;7(10):1557-1574. 2017 [PMID: 28979843] (Bioassay, Human) Bioassay Human
S Järnum, A Runström, R Bockermann, L Winstedt, M Crispin, C Kjellman Enzymatic inactivation of endogenous IgG by IdeS enhances�therapeutic antibody efficacy Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28533435] (Bioassay, Human) Bioassay Human
U Wessels, E Schick, M Ritter, F Kowalewsky, J Heinrich, K Stubenrauc Novel drug and soluble target tolerant antidrug antibody assay for therapeutic antibodies bearing the P329G mutation Bioanalysis, 2017;0(0):. 2017 [PMID: 28517957] (SPR, Human) SPR Human
Zhang X, Olsen H, Chen S, So E, Zhou H, Burch E, Merigeon E, Block D, Strome S Anti-CD20 Antibody with Multimerized Fc Domains: A Novel Strategy To Deplete B Cells and Augment Treatment of Autoimmune Disease. J Immunol, 2016;196(3):1165-76. 2016 [PMID: 26695368] (Surface Plasmon Resonance, N/A) Surface Plasmon Resonance N/A
McEnaney P, Fitzgerald K, Zhang A, Douglass E, Shan W, Balog A, Kolesnikova M, Spiegel D Chemically synthesized molecules with the targeting and effector functions of antibodies. J Am Chem Soc, 2014;136(52):18034-43. 2014 [PMID: 25514603] (Binding Assay, N/A) Binding Assay N/A
Yang Z, Ramsey J, Hamza T, Zhang Y, Li S, Yfantis H, Lee D, Hernandez L, Seghezzi W, Furneisen J, Davis N, Therien A, Feng H Mechanisms of protection against Clostridium difficile infection by the monoclonal antitoxin antibodies actoxumab and bezlotoxumab. Infect Immun, 2015;83(2):822-31. 2015 [PMID: 25486992] (Bioassay, Human) Bioassay Human
Meuris L, Santens F, Elson G, Festjens N, Boone M, Dos Santos A, Devos S, Rousseau F, Plets E, Houthuys E, Malinge P, Magistrelli G, Cons L, Chatel L, Devreese B, Callewaert N GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins. Nat Biotechnol, 2014;32(5):485-9. 2014 [PMID: 24752077] (Bioassay, Human) Bioassay Human
Xie J, Yamniuk A, Borowski V, Kuhn R, Susulic V, Rex-Rabe S, Yang X, Zhou X, Zhang Y, Gillooly K, Brosius R, Ravishankar R, Waggie K, Mink K, Price L, Rehfuss R, Tamura J, An Y, Cheng L, Abramczyk B, Ignatovich O, Drew P, Grant S, Bryson J, Suchard S, Salter-Cid L, Nadler S, Suri A Engineering of a novel anti-CD40L domain antibody for treatment of autoimmune diseases. J Immunol, 2014;192(9):4083-92. 2014 [PMID: 24670803] (Bioassay, Human) Bioassay Human
Derer S, Glorius P, Schlaeth M, Lohse S, Klausz K, Muchhal U, Desjarlais J, Humpe A, Valerius T, Peipp M Increasing FcgammaRIIa affinity of an FcgammaRIII-optimized anti-EGFR antibody restores neutrophil-mediated cytotoxicity. MAbs, 2014;6(2):409-21. 2014 [PMID: 24492248] (Surface Plasmon Resonance, N/A) Surface Plasmon Resonance N/A
Show All 12 Publications.

Reviews for Fc gamma RI/CD64 (1257-FC) (0)

There are no reviews for Fc gamma RI/CD64 (1257-FC). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Fc gamma RI/CD64 (1257-FC) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for Fc gamma RI/CD64 (1257-FC)

Discover related pathways, diseases and genes to Fc gamma RI/CD64 (1257-FC). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Fc gamma RI/CD64 (1257-FC)

Discover more about diseases related to Fc gamma RI/CD64 (1257-FC).
 

Pathways for Fc gamma RI/CD64 (1257-FC)

View related products by pathway.

PTMs for Fc gamma RI/CD64 (1257-FC)

Learn more about PTMs related to Fc gamma RI/CD64 (1257-FC).

Blogs on Fc gamma RI/CD64

There are no specific blogs for Fc gamma RI/CD64, but you can read our latest blog posts.

Customers Who Bought This Also Bought

CD11b Antibody
NB110-89474
IL-6 [Unconjugated]
7270-IL-025/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Fc gamma RI/CD64 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol FCGR1A
Entrez
Uniprot