Recombinant Human Fc gamma RI/CD64 Protein, CF

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When Human IgG is coated at 0.5 µg/mL, Recombinant Human Fc gamma RI/CD64 binds with an estimated Kd <0.15 nM.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human Fc gamma RI/CD64 Protein, CF Summary

Details of Functionality
Measured by its ability to bind human IgG with an estimated Kd <0.15 nM.
Source
Mouse myeloma cell line, NS0-derived human Fc gamma RI/CD64 protein
Gln16-Pro288, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained: Gln16 predicted
Protein/Peptide Type
Recombinant Proteins
Gene
FCGR1A
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
45-55 kDa, reducing conditions
Publications
Read Publications using
1257-FC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fc gamma RI/CD64 Protein, CF

  • CD64 antigen
  • CD64
  • CD64a
  • Fc fragment of IgG, high affinity Ia, receptor (CD64)
  • Fc gamma RI
  • FCG1
  • Fc-gamma receptor I B2
  • Fc-gamma RI
  • Fc-gamma RIA
  • FcgammaRIa
  • FCGR1
  • FcgRI
  • FcgRIA
  • FCRI
  • FcRIA
  • FLJ18345
  • high affinity Ia, receptor for (CD64)
  • high affinity immunoglobulin gamma Fc receptor I
  • IgG Fc receptor I

Background

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors
(~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three highly homologous genes (A, B, and C) sharing 98% identity at the nucleotide level have been identified for the human CD64 group (1). Fc gamma  RI is transmembrane protein with three extracellular Ig-like domains, and it delivers an activating signal via the associated Fc R gamma accessory chain. The genes for Fc gamma  RIB and Fc gamma  RIC contain stop codons within their membrane proximal Ig-like domains indicating possible secreted receptors (1, 6). An mRNA splice variant of Fc gamma RIB has a deletion of the membrane-proximal Ig-like domain and encodes a putative transmembrane receptor (6). The high affinity recognition of IgG by Fc gamma  RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses (2). Fc gamma  RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils (1, 4). Its expression is up-regulated during bacterial infections and sepsis.

  1. Van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.
  6. Ernst, L. et al. (1998) Mol Immunol. 35:943.

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1257-FC
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Publications for Fc gamma RI/CD64 (1257-FC)(20)

We have publications tested in 3 confirmed species: Human, N/A, Porcine.

We have publications tested in 6 applications: Binding Assay, Bioassay, ELISA (Capture), ELISA Capture, Surface Plasmon Resonance, Surface Plasmon Resonance (SPR.


Filter By Application
Binding Assay
(1)
Bioassay
(10)
ELISA (Capture)
(1)
ELISA Capture
(2)
Surface Plasmon Resonance
(5)
Surface Plasmon Resonance (SPR
(1)
All Applications
Filter By Species
Human
(15)
N/A
(3)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using 1257-FC Applications Species
Y Kitanaga, D Yamajuku, S Kubo, K Nakamura, M Maeda, M Seki, Y Kaneko, F Kinugasa, T Morokata, Y Kondo, H Yoshinari, S Nakayamada, T Sumida, Y Tanaka Discovery of a novel Igbeta and FcgammaRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosus International immunopharmacology, 2021;101(0):108343. 2021 [PMID: 34781122] (ELISA Capture, Human) ELISA Capture Human
AW Wessel, MP Doyle, TB Engdahl, J Rodriguez, JE Crowe, MS Diamond Human Monoclonal Antibodies against NS1 Protein Protect against Lethal West Nile Virus Infection MBio, 2021;12(5):e0244021. 2021 [PMID: 34634945] (Bioassay, Human) Bioassay Human
EH Aitken, T Damelang, A Ortega-Paj, A Alemu, W Hasang, S Dini, HW Unger, M Ome-Kaius, MA Nielsen, A Salanti, J Smith, S Kent, PM Hogarth, BD Wines, JA Simpson, A Chung, SJ Rogerson Developing a multivariate prediction model of antibody features associated with protection of malaria-infected pregnant women from placental malaria Elife, 2021;10(0):. 2021 [PMID: 34181872] (Bioassay, Human) Bioassay Human
B Vanhove, O Duvaux, J Rousse, PJ Royer, G Evanno, C Ciron, E Lheriteau, L Vacher, N Gervois, R Oger, Y Jacques, S Conchon, A Salama, R Duchi, I Lagutina, A Perota, P Delahaut, M Ledure, M Paulus, RT So, CK Mok, R Bruzzone, M Bouillet, S Brouard, E Cozzi, C Galli, D Blanchard, JM Bach, JP Soulillou High neutralizing potency of swine glyco-humanized polyclonal antibodies against SARS-CoV-2 European Journal of Immunology, 2021;0(0):. 2021 [PMID: 33576494] (Bioassay, Porcine) Bioassay Porcine
AW Wessel, N Kose, RG Bombardi, V Roy, W Chantima, J Mongkolsap, MA Edeling, CA Nelson, I Bosch, G Alter, GR Screaton, DH Fremont, JE Crowe, MS Diamond Antibodies targeting epitopes on the cell-surface form of NS1 protect against Zika virus infection during pregnancy Nat Commun, 2020;11(1):5278. 2020 [PMID: 33077712] (ELISA Capture, Human) ELISA Capture Human
JN An, L Li, J Lee, SS Yu, JH Kim, J Lee, YC Kim, DK Kim, YK Oh, CS Lim, YS Kim, S Kim, SH Yang, JP Lee Urinary cMet as a prognostic marker in immunoglobulin A nephropathy J. Cell. Mol. Med., 2020;0(0):. 2020 [PMID: 32822114] (Bioassay, Human) Bioassay Human
PA Blundell, D Lu, M Wilkinson, A Dell, S Haslam, RJ Pleass Insertion of N-Terminal Hinge Glycosylation Enhances Interactions of the Fc Region of Human IgG1 Monomers with Glycan-Dependent Receptors and Blocks Hemagglutination by the Influenza Virus J. Immunol., 2019;0(0):. 2019 [PMID: 30683699] (ELISA (Capture), Human) ELISA (Capture) Human
T Oshima, H Miyashita, Y Ishimura, Y Ito, Y Tanaka, A Hori, T Kokubo, T Kurokawa Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey PLoS ONE, 2018;13(5):e0196422. 2018 [PMID: 29723247] (Bioassay) Bioassay
F Arce Varga, AJS Furness, K Litchfield, K Joshi, R Rosenthal, E Ghorani, I Solomon, MH Lesko, N Ruef, C Roddie, JY Henry, L Spain, A Ben Aissa, A Georgiou, YNS Wong, M Smith, D Strauss, A Hayes, D Nicol, T O'Brien, L Mårtensson, A Ljungars, I Teige, B Frendéus, , , , M Pule, T Marafioti, M Gore, J Larkin, S Turajlic, C Swanton, KS Peggs, SA Quezada Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies Cancer Cell, 2018;0(0):. 2018 [PMID: 29576375] (Surface Plasmon Resonance (SPR, Human) Surface Plasmon Resonance (SPR Human
KPM Geuijen, C Oppers-Tie, DF Egging, PJ Simons, L Boon, RBM Schasfoort, MHM Eppink Rapid screening of IgG quality attributes - effects on Fc receptor binding FEBS Open Bio, 2017;7(10):1557-1574. 2017 [PMID: 28979843] (Bioassay, Human) Bioassay Human
Show All 20 Publications.

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Bioinformatics

Gene Symbol FCGR1A
Uniprot