Recombinant Human Fc gamma RI/CD64 Protein, CF


When Human IgG is coated at 0.5 µg/mL, Recombinant Human Fc gamma RI/CD64 binds with an estimated Kd <0.15 nM.

Product Details

Reactivity HuSpecies Glossary
Applications Binding Activity

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Recombinant Human Fc gamma RI/CD64 Protein, CF Summary

Details of Functionality
Measured by its ability to bind human IgG with an estimated Kd <0.15 nM.
Mouse myeloma cell line, NS0-derived human Fc gamma RI/CD64 protein
Gln16-Pro288, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained: Gln16 predicted
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
45-55 kDa, reducing conditions
Read Publications using
1257-FC in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fc gamma RI/CD64 Protein, CF

  • CD64 antigen
  • CD64
  • CD64a
  • Fc fragment of IgG, high affinity Ia, receptor (CD64)
  • Fc gamma RI
  • FCG1
  • Fc-gamma receptor I B2
  • Fc-gamma RI
  • Fc-gamma RIA
  • FcgammaRIa
  • FCGR1
  • FcgRI
  • FcgRIA
  • FCRI
  • FcRIA
  • FLJ18345
  • high affinity Ia, receptor for (CD64)
  • high affinity immunoglobulin gamma Fc receptor I
  • IgG Fc receptor I


Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors
(~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three highly homologous genes (A, B, and C) sharing 98% identity at the nucleotide level have been identified for the human CD64 group (1). Fc gamma  RI is transmembrane protein with three extracellular Ig-like domains, and it delivers an activating signal via the associated Fc R gamma accessory chain. The genes for Fc gamma  RIB and Fc gamma  RIC contain stop codons within their membrane proximal Ig-like domains indicating possible secreted receptors (1, 6). An mRNA splice variant of Fc gamma RIB has a deletion of the membrane-proximal Ig-like domain and encodes a putative transmembrane receptor (6). The high affinity recognition of IgG by Fc gamma  RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses (2). Fc gamma  RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils (1, 4). Its expression is up-regulated during bacterial infections and sepsis.

  1. Van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.
  6. Ernst, L. et al. (1998) Mol Immunol. 35:943.

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Applications: Binding Activity

Publications for Fc gamma RI/CD64 (1257-FC)(14)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 5 applications: Binding Assay, Bioassay, ELISA (Capture), Surface Plasmon Resonance, Surface Plasmon Resonance (SPR.

Filter By Application
Binding Assay
ELISA (Capture)
Surface Plasmon Resonance
Surface Plasmon Resonance (SPR
All Applications
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All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using 1257-FC Applications Species
PA Blundell, D Lu, M Wilkinson, A Dell, S Haslam, RJ Pleass Insertion of N-Terminal Hinge Glycosylation Enhances Interactions of the Fc Region of Human IgG1 Monomers with Glycan-Dependent Receptors and Blocks Hemagglutination by the Influenza Virus J. Immunol., 2019;0(0):. 2019 [PMID: 30683699] (ELISA (Capture), Human) ELISA (Capture) Human
T Oshima, H Miyashita, Y Ishimura, Y Ito, Y Tanaka, A Hori, T Kokubo, T Kurokawa Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey PLoS ONE, 2018;13(5):e0196422. 2018 [PMID: 29723247] (Bioassay) Bioassay
F Arce Varga, AJS Furness, K Litchfield, K Joshi, R Rosenthal, E Ghorani, I Solomon, MH Lesko, N Ruef, C Roddie, JY Henry, L Spain, A Ben Aissa, A Georgiou, YNS Wong, M Smith, D Strauss, A Hayes, D Nicol, T O'Brien, L Mårtensson, A Ljungars, I Teige, B Frendéus,,,, M Pule, T Marafioti, M Gore, J Larkin, S Turajlic, C Swanton, KS Peggs, SA Quezada Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies Cancer Cell, 2018;0(0):. 2018 [PMID: 29576375] (Surface Plasmon Resonance (SPR, Human) Surface Plasmon Resonance (SPR Human
KPM Geuijen, C Oppers-Tie, DF Egging, PJ Simons, L Boon, RBM Schasfoort, MHM Eppink Rapid screening of IgG quality attributes - effects on Fc receptor binding FEBS Open Bio, 2017;7(10):1557-1574. 2017 [PMID: 28979843] (Bioassay, Human) Bioassay Human
S Järnum, A Runström, R Bockermann, L Winstedt, M Crispin, C Kjellman Enzymatic inactivation of endogenous IgG by IdeS enhances�therapeutic antibody efficacy Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28533435] (Bioassay, Human) Bioassay Human
U Wessels, E Schick, M Ritter, F Kowalewsky, J Heinrich, K Stubenrauc Novel drug and soluble target tolerant antidrug antibody assay for therapeutic antibodies bearing the P329G mutation Bioanalysis, 2017;0(0):. 2017 [PMID: 28517957] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Zhang X, Olsen H, Chen S, So E, Zhou H, Burch E, Merigeon E, Block D, Strome S Anti-CD20 Antibody with Multimerized Fc Domains: A Novel Strategy To Deplete B Cells and Augment Treatment of Autoimmune Disease. J Immunol, 2016;196(3):1165-76. 2016 [PMID: 26695368] (Surface Plasmon Resonance, N/A) Surface Plasmon Resonance N/A
McEnaney P, Fitzgerald K, Zhang A, Douglass E, Shan W, Balog A, Kolesnikova M, Spiegel D Chemically synthesized molecules with the targeting and effector functions of antibodies. J Am Chem Soc, 2014;136(52):18034-43. 2014 [PMID: 25514603] (Binding Assay, N/A) Binding Assay N/A
Yang Z, Ramsey J, Hamza T, Zhang Y, Li S, Yfantis H, Lee D, Hernandez L, Seghezzi W, Furneisen J, Davis N, Therien A, Feng H Mechanisms of protection against Clostridium difficile infection by the monoclonal antitoxin antibodies actoxumab and bezlotoxumab. Infect Immun, 2015;83(2):822-31. 2015 [PMID: 25486992] (Bioassay, Human) Bioassay Human
Meuris L, Santens F, Elson G, Festjens N, Boone M, Dos Santos A, Devos S, Rousseau F, Plets E, Houthuys E, Malinge P, Magistrelli G, Cons L, Chatel L, Devreese B, Callewaert N GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins. Nat Biotechnol, 2014;32(5):485-9. 2014 [PMID: 24752077] (Bioassay, Human) Bioassay Human
Show All 14 Publications.

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Gene Symbol FCGR1A