Fc gamma RIII (CD16) Antibody [Unconjugated]

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Western blot shows lysates of human spleen tissue, human lung tissue, and human peripheral blood mononuclear cells (PBMC) . PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Fc gamma RIII (CD16) Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Fc gamma RIII (CD16) Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIB (R&D Systems, Catalog # 1597-FC)
Thr20-Gln208
Accession # O75015
Specificity
Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs and Western blots. In these formats, approximately 5% cross‑reactivity with recombinant human Fc gamma  RIIA is observed and less than 2% cross-reactivity with recombinant mouse CD16 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FCGR3B
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction
  • Western Blot 2 ug/mL
Publications
Read Publications using
AF1597 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RIII (CD16) Antibody [Unconjugated]

  • CD16
  • CD16A
  • Fc fragment of IgG receptor IIIa
  • Fc gamma RIII
  • FCG3
  • FCGR3
  • FcgRIII
  • FCR-10
  • FCRIII
  • FCRIIIA
  • IGFR3
  • IMD20

Background

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1 - 3). These receptors function in the activation or inhibition of immune responses. The activating-type receptor either has, or associates non-covalently with an accessory subunit (FcR gamma or zeta  chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular domains of Fc gamma RIIIA and B share 97% amino acid sequence homology. Whereas Fc gamma RIIIA is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells, and Langerhans cells, Fc gamma RIIIB is selectively expressed in neutrophils and eosinophils. Signaling through Fc gamma RIIIA results in oxidative burst, cytokine release and phagocytosis by macrophages, antibody-dependent cellular cytotoxicity by natural killer cells and degranulation of mast cells. By contrast, Fc gamma RIIIB is a decoy receptor that binds IgG complexes without triggering activation. Soluble Fc gamma RIIIB has a regulatory role in inflammatory processes (4). It interacts with complement receptors CR3 and CR4 on monocytes to induce the production of pro-inflammatory cytokines.

  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
  2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Gauchat, G.J. et al. (1996) J. Immunol. 157:1184.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fc gamma RIII (CD16) Antibody (AF1597)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow Cytometry, ICC, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
Western Blot
(4)
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Human
(6)
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Showing Publications 1 - 6 of 6.
Publications using AF1597 Applications Species
KR Patel, MC Rodriguez, WW Lorenz, EM Mace, AW Barb Fc &gamma receptor IIIa / CD16a processing correlates with the expression of glycan-related genes in human natural killer cells The Journal of Biological Chemistry, 2020;0(0):. 2020 [PMID: 33310702] (Western Blot, Human) Western Blot Human
OS Qureshi, TF Rowley, F Junker, SJ Peters, S Crilly, J Compson, A Eddleston, H Björkelund, K Greenslade, M Parkinson, NL Davies, R Griffin, TL Pither, K Cain, L Christodou, L Staelens, E Ward, J Tibbitts, A Kiessling, B Smith, FR Brennan, M Malmqvist, F Fallah-Ara, DP Humphreys Multivalent Fc?-receptor engagement by a hexameric Fc-fusion protein triggers Fc?-receptor internalisation and modulation of Fc?-receptor functions Sci Rep, 2017;7(1):17049. 2017 [PMID: 29213127] (Western Blot, Human) Western Blot Human
G Shen, S Krienke, P Schiller, A Nie beta en, S Neu, V Eckstein, M Schiller, HM Lorenz, LO Tykocinski Microvesicles released by apoptotic human neutrophils suppress proliferation and IL-2/ IL-2 receptor expression of resting T helper cells Eur. J. Immunol, 2017;0(0):. 2017 [PMID: 28295230] (Western Blot, Human) Western Blot Human
Chauhan A, Chen C, Moore T, DiPaolo R Induced expression of FcgammaRIIIa (CD16a) on CD4+ T cells triggers generation of IFN-gammahigh subset. J Biol Chem, 2015;290(8):5127-40. 2015 [PMID: 25556651] (Flow Cytometry, Human) Flow Cytometry Human
Chauhan AK, Moore TL Immune complexes and late complement proteins trigger activation of Syk tyrosine kinase in human CD4(+) T cells. Clin. Exp. Immunol., 2012;167(2):235-45. 2012 [PMID: 22235999] (ICC, Human) ICC Human
Bergin DA, Reeves EP, Meleady P, Henry M, McElvaney OJ, Carroll TP, Condron C, Chotirmall SH, Clynes M, O'Neill SJ, McElvaney NG Alpha-1 Antitrypsin regulates human neutrophil chemotaxis induced by soluble immune complexes and IL-8. J. Clin. Invest., 2010;120(12):4236-50. 2010 [PMID: 21060150] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol FCGR3B
Uniprot