MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." alt="RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Fc gamma RIII (CD16) Monoclonal Antibody (Catalog # MAB19601, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_thumb" />
HAF017). A specific band was detected for Fc gamma RIII (CD16) at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human spleen tissue, human lung tissue, and human peripheral blood mononuclear cells (PBMC) . PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Fc gamma RIII (CD16) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1597) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fc gamma RIII (CD16) at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1597-SP | |||
F0102B) and Mouse Anti-Human CD56 Alexa Fluor® 647-conjugated Monoclonal Antibody (FAB24081R). Staining was performed using our Staining Membrane-associated Proteins protocol." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human Fc gamma RIII A/B (CD16a/b) Monoclonal Antibody (Catalog # MAB2546) or (B) Mouse IgG2A isotype control antibody (MAB003) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0102B) and Mouse Anti-Human CD56 Alexa Fluor® 647-conjugated Monoclonal Antibody (FAB24081R). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />
HAF018). A specific band was detected for Fc gamma RIII (CD16) at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human lung tissue and human peripheral blood mononuclear cells (PBMCs). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Fc gamma RIII (CD16) Monoclonal Antibody (Catalog # MAB4325) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fc gamma RIII (CD16) at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4325-SP | |||
| Carrier Free | FAB4325V-100UG | |||
| Carrier Free | FAB4325U-100UG | |||
| Carrier Free | FAB4325T-100UG | |||
| Carrier Free | FAB4325S-100UG | |||
| Carrier Free | FAB4325R-100UG | |||
| Carrier Free | FAB4325N-100UG | |||
| Carrier Free | FAB4325G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21452-0.025mg | |||
| BSA Free | NBP3-21452-0.1mg | |||
NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Fc gamma RIII (CD16) was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Fc gamma RIII (CD16) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1960) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1960-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37713 | |||
MAB003) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human Fc gamma RIII (CD16) Monoclonal Antibody (Catalog # MAB43251) or (B) Mouse IgG2A isotype control antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB43251-SP | |||
| Carrier Free | FAB43251V-100UG | |||
| Carrier Free | FAB43251U-100UG | |||
| Carrier Free | FAB43251T-100UG | |||
| Carrier Free | FAB43251S-100UG | |||
| Carrier Free | FAB43251R-100UG | |||
| Carrier Free | FAB43251N-100UG | |||
| Carrier Free | FAB43251G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1960-SP | |||
042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system." class="big_thumb" />
MAB003) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (
MAB003) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # 
NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for 
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # ![Immunocytochemistry /Immunofluorescence: Fc gamma RIII (CD16) Antibody (3G8) - Chimeric - BSA Free [NBP3-21452] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells permeabilized with 0.15% Triton and stained with NBP3-21452 at 10 ug/ml for 1h followed by Alexa Fluor488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-21452, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-21452_rabbit-fc-gamma-riii-cd16-mab-3g8-chimeric-74202522141210.png "Immunocytochemistry /Immunofluorescence: Fc gamma RIII (CD16) Antibody (3G8) - Chimeric - BSA Free [NBP3-21452] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells permeabilized with 0.15% Triton and stained with NBP3-21452 at 10 ug/ml for 1h followed by Alexa Fluor488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-21452, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor488 secondary antibody.")
![Flow Cytometry: Fc gamma RIII (CD16) Antibody (3G8) - Chimeric - BSA Free [NBP3-21452] - Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (isotype control, black line) or NBP3-21452 (blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-21452_rabbit-fc-gamma-riii-cd16-mab-3g8-chimeric-74202522113022.png)
![Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) [NB500-378] - Separation of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (concentration in sample 9 ug/ml, GAM APC, red-filled) from FasL transfected L5178Y cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).](http://images.novusbio.com/fullsize/Fc-gamma-RIII-CD16-Antibody-MEM-154-Flow-Cytometry-NB500-378-img0002.jpg "Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) [NB500-378] - Separation of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (concentration in sample 9 ug/ml, GAM APC, red-filled) from FasL transfected L5178Y cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).")
![Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) [NB500-378] - Flow cytometry surface staining pattern of human peripheral blood stained using Fc gamma RIII (CD16) Antibody (MEM-154) purified antibody (concentration in sample 2 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Fc-gamma-RIII-CD16-Antibody-MEM-154-Flow-Cytometry-NB500-378-img0001.jpg)
![Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) - BSA Free [NB500-378] - Staining of human peripheral blood cells with anti-human CD16 (MEM-154) purified, GAM/APC.](http://images.novusbio.com/fullsize/nb500-378_mouse-monoclonal-fc-gamma-riii-cd16-antibody-mem-154-17720241003218.jpg)
NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for 
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # 
![Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32127, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-298202414333631.jpg "Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32127, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32127) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-29820241435244.jpg)
![Immunocytochemistry/ Immunofluorescence: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunocytochemistry analysis of 293T cells labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/100 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-2920241192312.jpg)
