Fc gamma RIII (CD16) Antibody (275003) [Unconjugated]

Images

 
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Fc gamma RIII (CD16) Monoclonal Antibody (Catalog # MAB19601, filled histogram) or isotype control antibody (Catalog # MAB006, open ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
275003
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Fc gamma RIII (CD16) Antibody (275003) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma  RIII
Ala31-Thr215
Accession # Q5D5J5
Specificity
Detects mouse Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Fcrl3/CD16-2, rmFc gamma RIA or rhFc gamma RIIA is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
Fcgr3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publications using
MAB19601 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RIII (CD16) Antibody (275003) [Unconjugated]

  • CD16
  • CD16A
  • Fc fragment of IgG receptor IIIa
  • Fc gamma RIII
  • FCG3
  • FCGR3
  • FcgRIII
  • FCR-10
  • FCRIII
  • FCRIIIA
  • IGFR3
  • IMD20

Background

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8‑10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6‑10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Mouse CD16 is encoded by a single gene. The protein product is a type I transmembrane protein having two extracellular Ig-like domains. It is expressed on a variety of myeloid and lymphoid cells (4) and associates with Fc R gamma to deliver an activating signal upon ligand binding (5). Mouse CD32 is closely related to mouse CD16 throughout its extracellular domain (95% amino acid sequence identity), but has a divergent cytoplasmic domain and functions as an inhibitory receptor. Together these proteins constitute an activating/inhibiting receptor pair to regulate immune responses (5).

  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fc gamma RIII (CD16) Antibody (MAB19601)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Bioassay, Flow Cytometry, Functional Assay, Neutralization.


Filter By Application
Bioassay
(1)
Flow Cytometry
(9)
Functional Assay
(2)
Neutralization
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(13)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using MAB19601 Applications Species
SK Panda, G Wigerblad, L Jiang, Y Jiménez-An, VS Iyer, Y Shen, SV Boddul, AO Guerreiro-, B Raposo, Z Kasza, F Wermeling IL-4 controls activated neutrophil FcgammaR2b expression and migration into inflamed joints Proc. Natl. Acad. Sci. U.S.A., 2020;117(6):3103-3113. 2020 [PMID: 31980518] (Flow Cytometry, Mouse) Flow Cytometry Mouse
P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity Cell Rep, 2019;27(11):3117-3123.e5. 2019 [PMID: 31189099] (Flow Cytometry, Human) Flow Cytometry Human
M Ott, E Avendaño-G, E Ullrich, C Dreyer, J Strauss, M Harden, M Schön, MP Schön, G Bernhardt, C Stadelmann, C Wegner, W Brück, S Nessler Laquinimod, a prototypic quinoline-3-carboxamide and aryl hydrocarbon receptor agonist, utilizes a CD155-mediated natural killer/dendritic cell interaction to suppress CNS autoimmunity J Neuroinflammation, 2019;16(1):49. 2019 [PMID: 30808363] (Flow Cytometry, Mouse) Flow Cytometry Mouse
S Arandjelov, JSA Perry, CD Lucas, KK Penberthy, TH Kim, M Zhou, DA Rosen, TY Chuang, AM Bettina, LS Shankman, AH Cohen, A Gaultier, TP Conrads, M Kim, MR Elliott, KS Ravichandr A noncanonical role for the engulfment gene ELMO1 in neutrophils that promotes inflammatory arthritis Nat. Immunol., 2019;20(2):141-151. 2019 [PMID: 30643265] (Flow Cytometry, Mouse) Flow Cytometry Mouse
JY Chu, B McCormick, G Mazelyte, M Michael, S Vermeren HoxB8 neutrophils replicate Fc? receptor and integrin-induced neutrophil signaling and functions J. Leukoc. Biol., 2018;0(0):. 2018 [PMID: 30211955] (Flow Cytometry, Mouse) Flow Cytometry Mouse
SN Syed, E Rau, M Ziegelmann, G Sogkas, B Brüne, RE Schmidt C5aR activation in the absence of C5a - a new disease mechanism of Autoimmune Hemolytic Anemia in mice Eur. J. Immunol., 2017;0(0):. 2017 [PMID: 29277896] (Flow Cytometry, Mouse) Flow Cytometry Mouse
CH Lehmann, A Baranska, GF Heidkamp, L Heger, K Neubert, JJ Lühr, A Hoffmann, KC Reimer, C Brückner, S Beck, M Seeling, M Kie beta ling, D Soulat, AB Krug, JV Ravetch, JH Leusen, F Nimmerjahn, D Dudziak DC subset-specific induction of T cell responses upon antigen uptake via Fc? receptors in vivo J. Exp. Med., 2017;0(0):. 2017 [PMID: 28389502] (Functional Assay, Mouse) Functional Assay Mouse
H Beutier, CM Gillis, B Iannascoli, O Godon, P England, R Sibilano, LL Reber, SJ Galli, MS Cragg, N Van Rooije, DA Mancardi, P Bruhns, F Jönsson IgG subclasses determine pathways of anaphylaxis in mice J. Allergy Clin. Immunol., 2017;139(1):269-280.e7. 2017 [PMID: 27246523] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Khodoun M, Kucuk Z, Strait R, Krishnamurthy D, Janek K, Clay C, Morris S, Finkelman F Rapid desensitization of mice with anti-FcgammaRIIb/FcgammaRIII mAb safely prevents IgG-mediated anaphylaxis. J Allergy Clin Immunol, 2013;132(6):1375-87. 2013 [PMID: 24139828] (Functional Assay, Mouse) Functional Assay Mouse
Albanesi M, Mancardi D, Macdonald L, Iannascoli B, Zitvogel L, Murphy A, Daeron M, Leusen J, Bruhns P Cutting edge: FcgammaRIII (CD16) and FcgammaRI (CD64) are responsible for anti-glycoprotein 75 monoclonal antibody TA99 therapy for experimental metastatic B16 melanoma. J Immunol, 2012;189(12):5513-7. 2012 [PMID: 23150715] (Bioassay, Mouse) Bioassay Mouse
Show All 14 Publications.

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Bioinformatics

Gene Symbol Fcgr3
Uniprot