Fc gamma RIII (CD16) Antibody (275003) [Allophycocyanin]

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Fc gamma RIII (CD16) APC-conjugated Monoclonal Antibody (Catalog # FAB19601A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 275003
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

Fc gamma RIII (CD16) Antibody (275003) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIII
  Ala31-Thr215
  Accession # Q5D5J5
• Specificity: Detects mouse Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Fcrl3/CD16-2, rmFc gamma RIA or rhFc gamma RIIA is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Fcgr3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RIII (CD16) Antibody (275003) [Allophycocyanin]

• CD16
• CD16A
• Fc fragment of IgG receptor IIIa
• Fc gamma RIII
• FCG3
• FCGR3
• FcgRIII
• FCR-10
• FCRIII
• FCRIIIA
• IGFR3
• IMD20

Background

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors
(~10^-8-10^-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10^-6-10^-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Mouse CD16 is encoded by a single gene. The protein product is a type I transmembrane protein having two extracellular Ig-like domains. It is expressed on a variety of myeloid and lymphoid cells (4) and associates with Fc R gamma to deliver an activating signal upon ligand binding (5). Mouse CD32 is closely related to mouse CD16 throughout its extracellular domain (95% amino acid sequence identity), but has a divergent cytoplasmic domain and functions as an inhibitory receptor. Together these proteins constitute an activating/inhibiting receptor pair to regulate immune responses (5).

1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
5. Ravetch, J. and L. Lanier (2000) Science 290:84.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fc gamma RIII (CD16) Antibody (FAB19601A)(7)

Publications using FAB19601A

• Wu, T;Yan, S;Yeh, YW;Fang, Y;Xiang, Z; Fc?R-dependent apoptosis regulates tissue persistence of mucosal and connective tissue mast cells European journal of immunology 2023-05-03 [PMID: 37137469] (Flow Cytometry, Mouse)
• T Deptuch, K Penderecka, M Kaczmarek, S Molenda, H Dams-Kozlo In vivo study of the immune response to bioengineered spider silk spheres Scientific Reports, 2022-08-05;12(1):13480. 2022-08-05 [PMID: 35931709] (Flow Cytometry, Mouse)
• JM Motta, VM Rumjanek, A Mantovani, M Locati Tumor-Released Products Promote Bone Marrow-Derived Macrophage Survival and Proliferation Biomedicines, 2021-10-04;9(10):. 2021-10-04 [PMID: 34680504] (Flow Cytometry, Mouse)
• Tomas Castro-Dopico, Thomas W. Dennison, John R. Ferdinand, Rebeccah J. Mathews, Aaron Fleming, Dean Clift et al. Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis Immunity 4/16/2019 [PMID: 30876876]
• Isao Kubota MafB enhances the phagocytic activity of RAW264.7 macrophages by promoting Fcgr3 expression Biochem. Biophys. Res. Commun., 2016-11-12;0(0):. 2016-11-12 [PMID: 27847322] (Flow Cytometry, Mouse)
• Investigation of the Immunomodulatory effect of Berberis vulgaris on core-pulsed dendritic cell vaccine BMC Complement Altern Med, 2016-08-30;16(1):325. 2016-08-30 [PMID: 27577059] (Flow Cytometry, Mouse)
• Cassard L, Jonsson F, Arnaud S, Daeron M Fcgamma receptors inhibit mouse and human basophil activation. J Immunol, 2012-08-20;189(6):2995-3006. 2012-08-20 [PMID: 22908332] (Flow Cytometry, Mouse)

Reviews for Fc gamma RIII (CD16) Antibody (FAB19601A) (2)

Average Rating: 3

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 01/21/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 23585480
• Species: Mouse

reviewed by: Verified Customer Flow Mouse 01/21/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 23585480
• Species: Mouse

Bioinformatics

• Gene Symbol: Fcgr3
• Uniprot:
  • Q5D5J5()